TGF-betas synthesized by RPE cells have autocrine activity on mesenchymal transformation and cell proliferation.

Sungchul Lee, S. H. Kim, H. J. Koh, O. W. Kwon

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The present study investigated the effects of transforming growth factor (TGF)-beta on retinal pigment epithelial (RPE) transformation in a simplified model and also whether or not TGF-beta exhibits similar proliferation effects on transformed RPE cells that it has on primary RPE cells. Furthermore, we examined the cell proliferation effects of RPE-conditioned medium (CM). A vertical wound measuring 2 mm in diameter was made on primary RPE monolayers. The expression of alpha-smooth muscle actin (SMA) by the cells located at the wound edges was observed using a confocal microscope under immunofluorescent staining. Cell proliferation was measured by incorporating 3H-thymidine into DNA. The presence of alpha-SMA was observed in the cells within the wound after treatment with TGF-beta2, while negative expression was observed in control cells. TGF-betas inhibited the proliferation of the primary cultures of RPE cells in a dose-dependent manner, but the spindle-shaped late-passaged RPE cells were not inhibited by these growth factors. The medium conditioned by RPE cells stimulated the proliferation of subconjunctival fibroblasts and inhibited the proliferation of primary RPE cells, in a manner similar to TGF-beta. These findings demonstrate that TGF-beta-stimulated RPE cells may evoke proliferative vitreoretinopathy through mesenchymal transformation and cell proliferation.

Original languageEnglish
Pages (from-to)271-277
Number of pages7
JournalYonsei medical journal
Volume42
Issue number3
DOIs
Publication statusPublished - 2001 Jan 1

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Retinal Pigments
Transforming Growth Factor beta
Epithelial Cells
Cell Proliferation
Conditioned Culture Medium
Actins
Wounds and Injuries
Transforming Growth Factor beta2
Proliferative Vitreoretinopathy
Thymidine
Smooth Muscle Myocytes
Smooth Muscle
Intercellular Signaling Peptides and Proteins
Fibroblasts
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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abstract = "The present study investigated the effects of transforming growth factor (TGF)-beta on retinal pigment epithelial (RPE) transformation in a simplified model and also whether or not TGF-beta exhibits similar proliferation effects on transformed RPE cells that it has on primary RPE cells. Furthermore, we examined the cell proliferation effects of RPE-conditioned medium (CM). A vertical wound measuring 2 mm in diameter was made on primary RPE monolayers. The expression of alpha-smooth muscle actin (SMA) by the cells located at the wound edges was observed using a confocal microscope under immunofluorescent staining. Cell proliferation was measured by incorporating 3H-thymidine into DNA. The presence of alpha-SMA was observed in the cells within the wound after treatment with TGF-beta2, while negative expression was observed in control cells. TGF-betas inhibited the proliferation of the primary cultures of RPE cells in a dose-dependent manner, but the spindle-shaped late-passaged RPE cells were not inhibited by these growth factors. The medium conditioned by RPE cells stimulated the proliferation of subconjunctival fibroblasts and inhibited the proliferation of primary RPE cells, in a manner similar to TGF-beta. These findings demonstrate that TGF-beta-stimulated RPE cells may evoke proliferative vitreoretinopathy through mesenchymal transformation and cell proliferation.",
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TGF-betas synthesized by RPE cells have autocrine activity on mesenchymal transformation and cell proliferation. / Lee, Sungchul; Kim, S. H.; Koh, H. J.; Kwon, O. W.

In: Yonsei medical journal, Vol. 42, No. 3, 01.01.2001, p. 271-277.

Research output: Contribution to journalArticle

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