We evaluated the role of oxidative stress in diabetic nephropathy by measuring intracellular reactive oxygen species (ROS) and redox-sensitive transcription factors in isolated peripheral mononuclear cells (PBMC) in 66 diabetic patients with or without diabetic nephropathy (Groups III and II, respectively) and 49 normal controls (Group I). Stimulated ROS was significantly higher in Group III compared to Group II (increment of H2O2-induced ROS production: 21.8 ± 2.2% vs. 11.1 ± 2.0%; increment of PMA-induced ROS production 23.5 ± 4.5% vs. 21.6 ± 2.2%; both respectively), and the activity of nuclear factor-kappa B (NF-κB) and activator protein-1 (AP-1), but not specificity protein 1 (Sp1) was significantly higher in Group III than in Group II (2.53-fold vs. 2.0-fold vs. 1.43-fold, respectively). Both PBMC- and urinary TGF-β1 levels were higher in Group III than Group II (3.23 ± 0.39 ng/g vs. 1.99 ± 0.68 ng/g in PBMCs, 16.88 ± 6.84 (ng/g Cr) vs. 5.61 ± 1.57 (ng/g Cr) in urine, both respectively), and they correlated with the activity of NF-κB and AP-1 and 24-h urine albumin excretion (UAE). Increased intracellular ROS generation in PBMCs of diabetic patients is involved in the pathogenesis of diabetic nephropathy via activation NF-κB and AP-1 and an increased expression of TGF-β1.
Bibliographical noteFunding Information:
This work was supported by a faculty research grant of Department of Internal Medicine, Yonsei University, College of Medicine and by Korean Diabetes Association for 2000.
All Science Journal Classification (ASJC) codes
- Internal Medicine
- Endocrinology, Diabetes and Metabolism