The Arabidopsis sn-1-specific mitochondrial acylhydrolase AtDLAH is positively correlated with seed viability

Young Sam Seo, Eun Yu Kim, Woo Taek Kim

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17 Citations (Scopus)

Abstract

Lipid-derived molecules produced by acylhydrolases play important roles in the regulation of diverse cellular functions in plants. In Arabidopsis, the DAD1-like phospholipase A1 family consists of 12 members, all of which possess a lipase 3 domain. In this study, the biochemical and cellular functions of AtDLAH, an Arabidopsis thaliana DAD1-like acylhydrolase, were examined. Bacterially expressed AtDLAH contained phospholipase A1 activity for catalysing the hydrolysis of phospholipids at the sn-1 position. However, AtDLAH displayed an even stronger preference for 1-lysophosphatidylcholine, 1-monodiacylglycerol, and phosphatidic acid, suggesting that AtDLAH is a sn-1-specific acylhydrolase. The AtDLAH gene was highly expressed in young seedlings, and its encoded protein was exclusively localized to the mitochondria. AtDLAH-overexpressing transgenic seeds (35S:AtDLAH) were markedly tolerant to accelerated-ageing treatment and thus had higher germination percentages than wild-type seeds. In contrast, the atdlah loss-of-function knockout mutant seeds were hypersusceptible to accelerated-ageing conditions. The 35S:AtDLAH seeds, as opposed to the atdlah seeds, exhibited a dark red staining pattern following tetrazolium treatment under both normal and accelerated-ageing conditions, suggesting that AtDLAH expression is positively correlated with seed viability. The enhanced viability of 35S:AtDLAH seeds was accompanied by more densely populated epidermal cells, lower levels of accumulated lipid hydroperoxides, and higher levels of polar lipids as compared with wild-type and atdlah mutant seeds. These results suggest that AtDLAH, a mitochondrial-localized sn-1-specific acylhydrolase, plays an important role in Arabidopsis seed viability.

Original languageEnglish
Pages (from-to)5683-5698
Number of pages16
JournalJournal of experimental botany
Volume62
Issue number15
DOIs
Publication statusPublished - 2011 Nov

Bibliographical note

Funding Information:
This work was supported by grants from the Technology Development Program for Agriculture and Forestry (Project no. 309017-5 funded by the Ministry for Agriculture, Forestry and Fisheries, Republic of Korea), the National Research Foundation (Project no. 2009-0078317 funded by the Ministry of Education, Science, and Technology, Republic of Korea), and the National Center for GM Crops (Project no. PJ008152 of the Next Generation BioGreen 21 Program funded by the Rural Development Administration, Republic of Korea) to WTK.

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

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