The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard

Lifeng Peng, Eugene A. Kapp, David Fenyö, Min Seok Kwon, Pu Jiang, Songfeng Wu, Ying Jiang, Marie Isabel Aguilar, Nikhat Ahmed, Mark S. Baker, Zongwei Cai, Yu Ju Chen, Phan Van Chi, Maxey C.M. Chung, Fuchu He, Alice C.L. Len, Pao Chi Liao, Kazuyuki Nakamura, Sai Ming Ngai, Young-Ki Paik & 12 others Tai Long Pan, Terence C.W. Poon, Ghasem Hosseini Salekdeh, Richard J. Simpson, Ravi Sirdeshmukh, Chantragan Srisomsap, Jisnuson Svasti, Yu Chang Tyan, Florian S. Dreyer, Danyl McLauchlan, Pisana Rawson, T. William Jordan

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse liver that contains endoplasmic reticulum and other organelles. In this study, we present the strategy used for the preparation and initial characterization of the membrane sample, including validation that the carbonate-washing step enriches for integral and lipid-anchored membrane proteins. Analysis of 17 independent data sets from five types of proteomic workflows is in progress.

Original languageEnglish
Pages (from-to)4142-4148
Number of pages7
JournalProteomics
Volume10
Issue number22
DOIs
Publication statusPublished - 2010 Nov 1

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Oceania
Carbonates
Proteome
Proteomics
Membranes
Lipid-Linked Proteins
Subcellular Fractions
Workflow
Washing
Endoplasmic Reticulum
Organelles
Liver
Membrane Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Peng, Lifeng ; Kapp, Eugene A. ; Fenyö, David ; Kwon, Min Seok ; Jiang, Pu ; Wu, Songfeng ; Jiang, Ying ; Aguilar, Marie Isabel ; Ahmed, Nikhat ; Baker, Mark S. ; Cai, Zongwei ; Chen, Yu Ju ; Van Chi, Phan ; Chung, Maxey C.M. ; He, Fuchu ; Len, Alice C.L. ; Liao, Pao Chi ; Nakamura, Kazuyuki ; Ngai, Sai Ming ; Paik, Young-Ki ; Pan, Tai Long ; Poon, Terence C.W. ; Hosseini Salekdeh, Ghasem ; Simpson, Richard J. ; Sirdeshmukh, Ravi ; Srisomsap, Chantragan ; Svasti, Jisnuson ; Tyan, Yu Chang ; Dreyer, Florian S. ; McLauchlan, Danyl ; Rawson, Pisana ; Jordan, T. William. / The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard. In: Proteomics. 2010 ; Vol. 10, No. 22. pp. 4142-4148.
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abstract = "The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse liver that contains endoplasmic reticulum and other organelles. In this study, we present the strategy used for the preparation and initial characterization of the membrane sample, including validation that the carbonate-washing step enriches for integral and lipid-anchored membrane proteins. Analysis of 17 independent data sets from five types of proteomic workflows is in progress.",
author = "Lifeng Peng and Kapp, {Eugene A.} and David Feny{\"o} and Kwon, {Min Seok} and Pu Jiang and Songfeng Wu and Ying Jiang and Aguilar, {Marie Isabel} and Nikhat Ahmed and Baker, {Mark S.} and Zongwei Cai and Chen, {Yu Ju} and {Van Chi}, Phan and Chung, {Maxey C.M.} and Fuchu He and Len, {Alice C.L.} and Liao, {Pao Chi} and Kazuyuki Nakamura and Ngai, {Sai Ming} and Young-Ki Paik and Pan, {Tai Long} and Poon, {Terence C.W.} and {Hosseini Salekdeh}, Ghasem and Simpson, {Richard J.} and Ravi Sirdeshmukh and Chantragan Srisomsap and Jisnuson Svasti and Tyan, {Yu Chang} and Dreyer, {Florian S.} and Danyl McLauchlan and Pisana Rawson and Jordan, {T. William}",
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Peng, L, Kapp, EA, Fenyö, D, Kwon, MS, Jiang, P, Wu, S, Jiang, Y, Aguilar, MI, Ahmed, N, Baker, MS, Cai, Z, Chen, YJ, Van Chi, P, Chung, MCM, He, F, Len, ACL, Liao, PC, Nakamura, K, Ngai, SM, Paik, Y-K, Pan, TL, Poon, TCW, Hosseini Salekdeh, G, Simpson, RJ, Sirdeshmukh, R, Srisomsap, C, Svasti, J, Tyan, YC, Dreyer, FS, McLauchlan, D, Rawson, P & Jordan, TW 2010, 'The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard', Proteomics, vol. 10, no. 22, pp. 4142-4148. https://doi.org/10.1002/pmic.201000126

The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard. / Peng, Lifeng; Kapp, Eugene A.; Fenyö, David; Kwon, Min Seok; Jiang, Pu; Wu, Songfeng; Jiang, Ying; Aguilar, Marie Isabel; Ahmed, Nikhat; Baker, Mark S.; Cai, Zongwei; Chen, Yu Ju; Van Chi, Phan; Chung, Maxey C.M.; He, Fuchu; Len, Alice C.L.; Liao, Pao Chi; Nakamura, Kazuyuki; Ngai, Sai Ming; Paik, Young-Ki; Pan, Tai Long; Poon, Terence C.W.; Hosseini Salekdeh, Ghasem; Simpson, Richard J.; Sirdeshmukh, Ravi; Srisomsap, Chantragan; Svasti, Jisnuson; Tyan, Yu Chang; Dreyer, Florian S.; McLauchlan, Danyl; Rawson, Pisana; Jordan, T. William.

In: Proteomics, Vol. 10, No. 22, 01.11.2010, p. 4142-4148.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard

AU - Peng, Lifeng

AU - Kapp, Eugene A.

AU - Fenyö, David

AU - Kwon, Min Seok

AU - Jiang, Pu

AU - Wu, Songfeng

AU - Jiang, Ying

AU - Aguilar, Marie Isabel

AU - Ahmed, Nikhat

AU - Baker, Mark S.

AU - Cai, Zongwei

AU - Chen, Yu Ju

AU - Van Chi, Phan

AU - Chung, Maxey C.M.

AU - He, Fuchu

AU - Len, Alice C.L.

AU - Liao, Pao Chi

AU - Nakamura, Kazuyuki

AU - Ngai, Sai Ming

AU - Paik, Young-Ki

AU - Pan, Tai Long

AU - Poon, Terence C.W.

AU - Hosseini Salekdeh, Ghasem

AU - Simpson, Richard J.

AU - Sirdeshmukh, Ravi

AU - Srisomsap, Chantragan

AU - Svasti, Jisnuson

AU - Tyan, Yu Chang

AU - Dreyer, Florian S.

AU - McLauchlan, Danyl

AU - Rawson, Pisana

AU - Jordan, T. William

PY - 2010/11/1

Y1 - 2010/11/1

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