The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard

Lifeng Peng, Eugene A. Kapp, David Fenyö, Min Seok Kwon, Pu Jiang, Songfeng Wu, Ying Jiang, Marie Isabel Aguilar, Nikhat Ahmed, Mark S. Baker, Zongwei Cai, Yu Ju Chen, Phan Van Chi, Maxey C.M. Chung, Fuchu He, Alice C.L. Len, Pao Chi Liao, Kazuyuki Nakamura, Sai Ming Ngai, Young Ki PaikTai Long Pan, Terence C.W. Poon, Ghasem Hosseini Salekdeh, Richard J. Simpson, Ravi Sirdeshmukh, Chantragan Srisomsap, Jisnuson Svasti, Yu Chang Tyan, Florian S. Dreyer, Danyl McLauchlan, Pisana Rawson, T. William Jordan

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Abstract

The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse liver that contains endoplasmic reticulum and other organelles. In this study, we present the strategy used for the preparation and initial characterization of the membrane sample, including validation that the carbonate-washing step enriches for integral and lipid-anchored membrane proteins. Analysis of 17 independent data sets from five types of proteomic workflows is in progress.

Original languageEnglish
Pages (from-to)4142-4148
Number of pages7
JournalProteomics
Volume10
Issue number22
DOIs
Publication statusPublished - 2010 Nov 1

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Peng, L., Kapp, E. A., Fenyö, D., Kwon, M. S., Jiang, P., Wu, S., Jiang, Y., Aguilar, M. I., Ahmed, N., Baker, M. S., Cai, Z., Chen, Y. J., Van Chi, P., Chung, M. C. M., He, F., Len, A. C. L., Liao, P. C., Nakamura, K., Ngai, S. M., ... Jordan, T. W. (2010). The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard. Proteomics, 10(22), 4142-4148. https://doi.org/10.1002/pmic.201000126