The balneotherapy effect of hydrogen reduced water on UVB-mediated skin injury in hairless mice

Rosa Mistica Ignacio, Yang Suk Yoon, Ma Easter Joy Sajo, Cheol Su Kim, Dong Heui Kim, Soo-Ki Kim, Kyu Jae Lee

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Exposure to UVB radiation induced skin damage that results to increase risk of skin cancer. Despite the clinical importance of skin-induced damage, antioxidants imposed limited therapeutic success. Hydrogen molecule (H 2) has been known as a safe antioxidant in the prevention and therapeutic approach towards several diseases. Drinking hydrogen reduced water (HRW), inhalation of hydrogen gas, and injecting H2-dissolved saline are widely accepted to incorporate H2 in the body. However, there is no document about the beneficial effect of hydrogen water bath. Here, we investigated the effect of hydrogen bathing on the UVB-induced skin damage in hairless mice. For this, mice of the bathing group are allowed to freely swim on HRW, and let the HRW penetrate for 60 mins. Scoring of skin injury, reactive oxygen species (ROS) enzyme activity quantification, cytokine analysis, and ultrastructural change of corneocytes were measured after exposure to UVB radiation of 360-540 mJ/cm2. In summary, the bathing with HRW significantly reduced the levels of skin damage, as well as increased activity of glutathione peroxidase. Further, the effect of HRW on cytokine network in the skin after UVB exposure revealed that HRW significantly decreased the level of inflammatory cytokines such as IL-1β, IL-6, TNF-α and IFN-γ. Finally, scanning electron microscopy data revealed low number of defected corneocytes and ultrastructural changes, suggesting that HRW bathing would protect UV-induced cell damage.

Original languageEnglish
Pages (from-to)15-21
Number of pages7
JournalMolecular and Cellular Toxicology
Volume9
Issue number1
DOIs
Publication statusPublished - 2013 Mar 1

Fingerprint

Balneology
Hairless Mouse
Hydrogen
Skin
Water
Wounds and Injuries
Cytokines
Antioxidants
Radiation
Enzyme activity
Skin Neoplasms
Glutathione Peroxidase
Interleukin-1
Baths
Electron Scanning Microscopy
Inhalation
Drinking
Interleukin-6
Reactive Oxygen Species

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Toxicology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Ignacio, Rosa Mistica ; Yoon, Yang Suk ; Sajo, Ma Easter Joy ; Kim, Cheol Su ; Kim, Dong Heui ; Kim, Soo-Ki ; Lee, Kyu Jae. / The balneotherapy effect of hydrogen reduced water on UVB-mediated skin injury in hairless mice. In: Molecular and Cellular Toxicology. 2013 ; Vol. 9, No. 1. pp. 15-21.
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The balneotherapy effect of hydrogen reduced water on UVB-mediated skin injury in hairless mice. / Ignacio, Rosa Mistica; Yoon, Yang Suk; Sajo, Ma Easter Joy; Kim, Cheol Su; Kim, Dong Heui; Kim, Soo-Ki; Lee, Kyu Jae.

In: Molecular and Cellular Toxicology, Vol. 9, No. 1, 01.03.2013, p. 15-21.

Research output: Contribution to journalArticle

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AU - Yoon, Yang Suk

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