The blaOXA-23-associated transposons in the genome of Acinetobacter spp. represent an epidemiological situation of the species encountering carbapenems

Eun Jeong Yoon, Jung Ok Kim, Ji Woo Yang, Hwa Su Kim, Kwang Jun Lee, Seok Hoon Jeong, Hyukmin Lee, Kyungwon Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objectives: High rates of carbapenem resistance in the human pathogen Acinetobacter baumannii threaten public health and need to be scrutinized. Methods: A total of 356 A. baumannii and 50 non-baumannii Acinetobacter spp. (NBA) strains collected in 2013 throughout South Korea were studied. The type of blaOXA-23 transposon was determined by PCR mapping and molecular epidemiology was assessed by MLST. Twelve representative strains and two comparative A. baumannii were entirely sequenced by single-molecule real-time sequencing. Results: The carbapenem resistance rate was 88% in A. baumannii, mainly due to blaOXA-23, with five exceptional cases associatedwith ISAba1-blaOXA-51-like. The blaOXA-23 gene in A. baumannii was carried either by Tn2006 (44%) or Tn2009 (54%), with a few exceptions carried by Tn2008 (1.6%). Of the NBA strains, 14% were resistant to carbapenems, twowith blaOXA-58 and five with blaOXA-23 associated with Tn2006. The Tn2006-possessing strains belonged to various STs, whereas Tn2008- and Tn2009-possessing strains were limited to ST208 and ST191, respectively. The three transposons were oftenmultiplied in the chromosome, and the gene copy number and the carbapenem MICs presented linear relationships either very strongly for Tn2008 ormoderately for Tn2006 and Tn2009. Conclusions: The dissemination of Tn2006 was facilitated by its capability for intercellular transfer and that of Tn2009 was attributable to successful dissemination of the ST191 bacterial host carrying the transposon. Tn2008 was infrequent because of its insufficient ability to undergo intercellular transfer and the scarce bacterial host A. baumannii ST208. Gene amplification is an adaptivemechanismfor bacteria that encounter antimicrobial drugs.

Original languageEnglish
Pages (from-to)2708-2714
Number of pages7
JournalJournal of Antimicrobial Chemotherapy
Volume72
Issue number10
DOIs
Publication statusPublished - 2017 Oct 1

Fingerprint

Acinetobacter baumannii
Acinetobacter
Carbapenems
Genome
Republic of Korea
Gene Dosage
Molecular Epidemiology
Gene Amplification
Public Health
Chromosomes
Bacteria
Polymerase Chain Reaction
Pharmaceutical Preparations
Genes

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

Cite this

@article{ba5acb79e4624fb2bee7c6730b4cba31,
title = "The blaOXA-23-associated transposons in the genome of Acinetobacter spp. represent an epidemiological situation of the species encountering carbapenems",
abstract = "Objectives: High rates of carbapenem resistance in the human pathogen Acinetobacter baumannii threaten public health and need to be scrutinized. Methods: A total of 356 A. baumannii and 50 non-baumannii Acinetobacter spp. (NBA) strains collected in 2013 throughout South Korea were studied. The type of blaOXA-23 transposon was determined by PCR mapping and molecular epidemiology was assessed by MLST. Twelve representative strains and two comparative A. baumannii were entirely sequenced by single-molecule real-time sequencing. Results: The carbapenem resistance rate was 88{\%} in A. baumannii, mainly due to blaOXA-23, with five exceptional cases associatedwith ISAba1-blaOXA-51-like. The blaOXA-23 gene in A. baumannii was carried either by Tn2006 (44{\%}) or Tn2009 (54{\%}), with a few exceptions carried by Tn2008 (1.6{\%}). Of the NBA strains, 14{\%} were resistant to carbapenems, twowith blaOXA-58 and five with blaOXA-23 associated with Tn2006. The Tn2006-possessing strains belonged to various STs, whereas Tn2008- and Tn2009-possessing strains were limited to ST208 and ST191, respectively. The three transposons were oftenmultiplied in the chromosome, and the gene copy number and the carbapenem MICs presented linear relationships either very strongly for Tn2008 ormoderately for Tn2006 and Tn2009. Conclusions: The dissemination of Tn2006 was facilitated by its capability for intercellular transfer and that of Tn2009 was attributable to successful dissemination of the ST191 bacterial host carrying the transposon. Tn2008 was infrequent because of its insufficient ability to undergo intercellular transfer and the scarce bacterial host A. baumannii ST208. Gene amplification is an adaptivemechanismfor bacteria that encounter antimicrobial drugs.",
author = "Yoon, {Eun Jeong} and Kim, {Jung Ok} and Yang, {Ji Woo} and Kim, {Hwa Su} and Lee, {Kwang Jun} and Jeong, {Seok Hoon} and Hyukmin Lee and Kyungwon Lee",
year = "2017",
month = "10",
day = "1",
doi = "10.1093/jac/dkx205",
language = "English",
volume = "72",
pages = "2708--2714",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",
publisher = "Oxford University Press",
number = "10",

}

The blaOXA-23-associated transposons in the genome of Acinetobacter spp. represent an epidemiological situation of the species encountering carbapenems. / Yoon, Eun Jeong; Kim, Jung Ok; Yang, Ji Woo; Kim, Hwa Su; Lee, Kwang Jun; Jeong, Seok Hoon; Lee, Hyukmin; Lee, Kyungwon.

In: Journal of Antimicrobial Chemotherapy, Vol. 72, No. 10, 01.10.2017, p. 2708-2714.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The blaOXA-23-associated transposons in the genome of Acinetobacter spp. represent an epidemiological situation of the species encountering carbapenems

AU - Yoon, Eun Jeong

AU - Kim, Jung Ok

AU - Yang, Ji Woo

AU - Kim, Hwa Su

AU - Lee, Kwang Jun

AU - Jeong, Seok Hoon

AU - Lee, Hyukmin

AU - Lee, Kyungwon

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Objectives: High rates of carbapenem resistance in the human pathogen Acinetobacter baumannii threaten public health and need to be scrutinized. Methods: A total of 356 A. baumannii and 50 non-baumannii Acinetobacter spp. (NBA) strains collected in 2013 throughout South Korea were studied. The type of blaOXA-23 transposon was determined by PCR mapping and molecular epidemiology was assessed by MLST. Twelve representative strains and two comparative A. baumannii were entirely sequenced by single-molecule real-time sequencing. Results: The carbapenem resistance rate was 88% in A. baumannii, mainly due to blaOXA-23, with five exceptional cases associatedwith ISAba1-blaOXA-51-like. The blaOXA-23 gene in A. baumannii was carried either by Tn2006 (44%) or Tn2009 (54%), with a few exceptions carried by Tn2008 (1.6%). Of the NBA strains, 14% were resistant to carbapenems, twowith blaOXA-58 and five with blaOXA-23 associated with Tn2006. The Tn2006-possessing strains belonged to various STs, whereas Tn2008- and Tn2009-possessing strains were limited to ST208 and ST191, respectively. The three transposons were oftenmultiplied in the chromosome, and the gene copy number and the carbapenem MICs presented linear relationships either very strongly for Tn2008 ormoderately for Tn2006 and Tn2009. Conclusions: The dissemination of Tn2006 was facilitated by its capability for intercellular transfer and that of Tn2009 was attributable to successful dissemination of the ST191 bacterial host carrying the transposon. Tn2008 was infrequent because of its insufficient ability to undergo intercellular transfer and the scarce bacterial host A. baumannii ST208. Gene amplification is an adaptivemechanismfor bacteria that encounter antimicrobial drugs.

AB - Objectives: High rates of carbapenem resistance in the human pathogen Acinetobacter baumannii threaten public health and need to be scrutinized. Methods: A total of 356 A. baumannii and 50 non-baumannii Acinetobacter spp. (NBA) strains collected in 2013 throughout South Korea were studied. The type of blaOXA-23 transposon was determined by PCR mapping and molecular epidemiology was assessed by MLST. Twelve representative strains and two comparative A. baumannii were entirely sequenced by single-molecule real-time sequencing. Results: The carbapenem resistance rate was 88% in A. baumannii, mainly due to blaOXA-23, with five exceptional cases associatedwith ISAba1-blaOXA-51-like. The blaOXA-23 gene in A. baumannii was carried either by Tn2006 (44%) or Tn2009 (54%), with a few exceptions carried by Tn2008 (1.6%). Of the NBA strains, 14% were resistant to carbapenems, twowith blaOXA-58 and five with blaOXA-23 associated with Tn2006. The Tn2006-possessing strains belonged to various STs, whereas Tn2008- and Tn2009-possessing strains were limited to ST208 and ST191, respectively. The three transposons were oftenmultiplied in the chromosome, and the gene copy number and the carbapenem MICs presented linear relationships either very strongly for Tn2008 ormoderately for Tn2006 and Tn2009. Conclusions: The dissemination of Tn2006 was facilitated by its capability for intercellular transfer and that of Tn2009 was attributable to successful dissemination of the ST191 bacterial host carrying the transposon. Tn2008 was infrequent because of its insufficient ability to undergo intercellular transfer and the scarce bacterial host A. baumannii ST208. Gene amplification is an adaptivemechanismfor bacteria that encounter antimicrobial drugs.

UR - http://www.scopus.com/inward/record.url?scp=85030680702&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85030680702&partnerID=8YFLogxK

U2 - 10.1093/jac/dkx205

DO - 10.1093/jac/dkx205

M3 - Article

C2 - 29091183

AN - SCOPUS:85030680702

VL - 72

SP - 2708

EP - 2714

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

IS - 10

ER -