The C-Domain of the NAC Transcription Factor ANAC019 Is Necessary for pH-Tuned DNA Binding through a Histidine Switch in the N-Domain

Mooseok Kang, Sangyeol Kim, Hyo Jung Kim, Pravesh Shrestha, Ji hye Yun, Bong Kwan Phee, Weon Tae Lee, Hong Gil Nam, Iksoo Chang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The affinity of transcription factors (TFs) for their target DNA is a critical determinant of gene expression. Whether the DNA-binding domain (DBD) of TFs alone can regulate binding affinity to DNA is an important question for identifying the design principle of TFs. We studied ANAC019, a member of the NAC TF family of proteins in Arabidopsis, and found a well-conserved histidine switch located in its DBD, which regulates both homodimerization and transcriptional control of the TF through H135 protonation. We found that the removal of a C-terminal intrinsically disordered region (IDR) in the TF abolished the pH-dependent binding of the N-terminal DBD to DNA. We propose a mechanism in which long-range electrostatic interactions between DNA and the negatively charged C-terminal IDR turns on the pH dependency of the DNA-binding affinity of the N-terminal DBD. Kang et al. find a histidine switch in the DNA-binding N-domain of the transcription factor ANAC019 that regulates both pH-dependent homodimerization and DNA binding. They propose that long-range electrostatic interactions between DNA and the negatively charged C-terminal turns on the pH dependency of the DNA-binding affinity of the N-terminal DNA-binding domain.

Original languageEnglish
Pages (from-to)1141-1150
Number of pages10
JournalCell Reports
Volume22
Issue number5
DOIs
Publication statusPublished - 2018 Jan 30

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Histidine
Transcription Factors
Switches
DNA
Coulomb interactions
Static Electricity
Arabidopsis Proteins
Protonation
Gene expression

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kang, Mooseok ; Kim, Sangyeol ; Kim, Hyo Jung ; Shrestha, Pravesh ; Yun, Ji hye ; Phee, Bong Kwan ; Lee, Weon Tae ; Nam, Hong Gil ; Chang, Iksoo. / The C-Domain of the NAC Transcription Factor ANAC019 Is Necessary for pH-Tuned DNA Binding through a Histidine Switch in the N-Domain. In: Cell Reports. 2018 ; Vol. 22, No. 5. pp. 1141-1150.
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The C-Domain of the NAC Transcription Factor ANAC019 Is Necessary for pH-Tuned DNA Binding through a Histidine Switch in the N-Domain. / Kang, Mooseok; Kim, Sangyeol; Kim, Hyo Jung; Shrestha, Pravesh; Yun, Ji hye; Phee, Bong Kwan; Lee, Weon Tae; Nam, Hong Gil; Chang, Iksoo.

In: Cell Reports, Vol. 22, No. 5, 30.01.2018, p. 1141-1150.

Research output: Contribution to journalArticle

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