The cDNA cloning and ontogeny of mouse α-synuclein

Lyhna Hong; Hyuk Wan Ko; B. J. Gwag; Eunhye Joe; Seoungoh Lee; Young Tae Kim; Yoo Hun Suh

doi:10.1097/00001756-199804200-00051

The cDNA cloning and ontogeny of mouse α-synuclein

Lyhna Hong, Hyuk Wan Ko, B. J. Gwag, Eunhye Joe, Seoungoh Lee, Young Tae Kim, Yoo Hun Suh

Department of Biochemistry

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

ALPHA-SYNUCLEIN has been implicated in the pathogenesis of Parkinson's disease. To investigate the role of α-synuclein in the brain, the cDNA clone encoding the mouse cognate of the human α-synuclein was isolated from a mouse brain cDNA library. The open reading frame coded for 140 amino acids that share 95% identity with human α-synuclein. Northern blot analysis showed that α-synuclein mRNA was primarily expressed in brain and spleen of adult mouse. In situ hybridization histochemistry revealed the highest expression of α-synuclein mRNA in the hippocampal formation and neocortex of the adult mouse. α-Synuclein mRNA expression in the brain was first observed in the hippocampus and neocortex on postnatal day 1. Levels of α-synuclein mRNA in these forebrain areas were nearly maximal at postnatal day 7 and remained relatively high until the adult stage. α-Synuclein mRNA was expressed in the liver transiently during embryogenesis.

Original language	English
Pages (from-to)	1239-1243
Number of pages	5
Journal	NeuroReport
Volume	9
Issue number	6
DOIs	https://doi.org/10.1097/00001756-199804200-00051
Publication status	Published - 1998 Apr 20

All Science Journal Classification (ASJC) codes

Neuroscience(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1097/00001756-199804200-00051

Cite this

@article{b125ba0c5b8243868bc84cfe6470d617,

title = "The cDNA cloning and ontogeny of mouse α-synuclein",

abstract = "ALPHA-SYNUCLEIN has been implicated in the pathogenesis of Parkinson's disease. To investigate the role of α-synuclein in the brain, the cDNA clone encoding the mouse cognate of the human α-synuclein was isolated from a mouse brain cDNA library. The open reading frame coded for 140 amino acids that share 95% identity with human α-synuclein. Northern blot analysis showed that α-synuclein mRNA was primarily expressed in brain and spleen of adult mouse. In situ hybridization histochemistry revealed the highest expression of α-synuclein mRNA in the hippocampal formation and neocortex of the adult mouse. α-Synuclein mRNA expression in the brain was first observed in the hippocampus and neocortex on postnatal day 1. Levels of α-synuclein mRNA in these forebrain areas were nearly maximal at postnatal day 7 and remained relatively high until the adult stage. α-Synuclein mRNA was expressed in the liver transiently during embryogenesis.",

author = "Lyhna Hong and Ko, {Hyuk Wan} and Gwag, {B. J.} and Eunhye Joe and Seoungoh Lee and Kim, {Young Tae} and Suh, {Yoo Hun}",

year = "1998",

month = apr,

day = "20",

doi = "10.1097/00001756-199804200-00051",

language = "English",

volume = "9",

pages = "1239--1243",

journal = "NeuroReport",

issn = "0959-4965",

publisher = "Lippincott Williams and Wilkins",

number = "6",

}

TY - JOUR

T1 - The cDNA cloning and ontogeny of mouse α-synuclein

AU - Hong, Lyhna

AU - Ko, Hyuk Wan

AU - Gwag, B. J.

AU - Joe, Eunhye

AU - Lee, Seoungoh

AU - Kim, Young Tae

AU - Suh, Yoo Hun

PY - 1998/4/20

Y1 - 1998/4/20

N2 - ALPHA-SYNUCLEIN has been implicated in the pathogenesis of Parkinson's disease. To investigate the role of α-synuclein in the brain, the cDNA clone encoding the mouse cognate of the human α-synuclein was isolated from a mouse brain cDNA library. The open reading frame coded for 140 amino acids that share 95% identity with human α-synuclein. Northern blot analysis showed that α-synuclein mRNA was primarily expressed in brain and spleen of adult mouse. In situ hybridization histochemistry revealed the highest expression of α-synuclein mRNA in the hippocampal formation and neocortex of the adult mouse. α-Synuclein mRNA expression in the brain was first observed in the hippocampus and neocortex on postnatal day 1. Levels of α-synuclein mRNA in these forebrain areas were nearly maximal at postnatal day 7 and remained relatively high until the adult stage. α-Synuclein mRNA was expressed in the liver transiently during embryogenesis.

AB - ALPHA-SYNUCLEIN has been implicated in the pathogenesis of Parkinson's disease. To investigate the role of α-synuclein in the brain, the cDNA clone encoding the mouse cognate of the human α-synuclein was isolated from a mouse brain cDNA library. The open reading frame coded for 140 amino acids that share 95% identity with human α-synuclein. Northern blot analysis showed that α-synuclein mRNA was primarily expressed in brain and spleen of adult mouse. In situ hybridization histochemistry revealed the highest expression of α-synuclein mRNA in the hippocampal formation and neocortex of the adult mouse. α-Synuclein mRNA expression in the brain was first observed in the hippocampus and neocortex on postnatal day 1. Levels of α-synuclein mRNA in these forebrain areas were nearly maximal at postnatal day 7 and remained relatively high until the adult stage. α-Synuclein mRNA was expressed in the liver transiently during embryogenesis.

UR - http://www.scopus.com/inward/record.url?scp=15144361589&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=15144361589&partnerID=8YFLogxK

U2 - 10.1097/00001756-199804200-00051

DO - 10.1097/00001756-199804200-00051

M3 - Article

C2 - 9601701

AN - SCOPUS:15144361589

VL - 9

SP - 1239

EP - 1243

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 6

ER -

The cDNA cloning and ontogeny of mouse α-synuclein

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this