The cell adhesion molecule L1 promotes gallbladder carcinoma progression in vitro and in vivo

Juyeon Jung, Yeon Sung Son, Hongryeol Park, Seong Kook Jeon, Jung Whoi Lee, Song Yi Choi, Jin Man Kim, Young-Guen Kwon, Hyo Jeong Hong, Jeong Ki Min

Research output: Contribution to journalArticle

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Abstract

Recent studies have demonstrated that the cell adhesion molecule, L1, is expressed in several malignant tumor types and its expression correlates with tumor progression and metastasis. However, the role of L1 in gallbladder carcinoma (GBC) remains unclear. Here, we demonstrate that L1 is expressed in GBC cells and plays an important role in the growth, motility, invasiveness, and adhesiveness of GBC cells. Specific depletion or overexpression of L1 in the GBC cell lines JCRB1033 and SNU-308, respectively, was achieved by lentivirus-mediated transduction and expression of an L1 mRNA-specific short hairpin RNA or full-length human L1. Stable depletion of L1 led to a significant decrease in GBC cell proliferation, migration and invasion, as well as decreased intracellular signaling through AKT and FAK. Overexpression of L1 in GBC cells enhanced these cellular activities. In a GBC xenograft nude mouse model, suppression of L1 markedly reduced tumor growth and increased the survival of tumor-bearing mice whereas L1 overexpression stimulated tumorigenicity. Taken together, these results suggest that L1 plays a crucial role in GBC progression and may be a novel therapeutic target in GBC treatment.

Original languageEnglish
Pages (from-to)945-952
Number of pages8
JournalOncology Reports
Volume25
Issue number4
DOIs
Publication statusPublished - 2011 Apr 1

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Neural Cell Adhesion Molecule L1
Gallbladder
Carcinoma
Neoplasms
In Vitro Techniques
Adhesiveness
Lentivirus
Growth
Heterografts
Nude Mice
Small Interfering RNA
Cell Movement
Cell Proliferation
Neoplasm Metastasis

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Jung, J., Son, Y. S., Park, H., Jeon, S. K., Lee, J. W., Choi, S. Y., ... Min, J. K. (2011). The cell adhesion molecule L1 promotes gallbladder carcinoma progression in vitro and in vivo. Oncology Reports, 25(4), 945-952. https://doi.org/10.3892/or.2011.1181
Jung, Juyeon ; Son, Yeon Sung ; Park, Hongryeol ; Jeon, Seong Kook ; Lee, Jung Whoi ; Choi, Song Yi ; Kim, Jin Man ; Kwon, Young-Guen ; Hong, Hyo Jeong ; Min, Jeong Ki. / The cell adhesion molecule L1 promotes gallbladder carcinoma progression in vitro and in vivo. In: Oncology Reports. 2011 ; Vol. 25, No. 4. pp. 945-952.
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abstract = "Recent studies have demonstrated that the cell adhesion molecule, L1, is expressed in several malignant tumor types and its expression correlates with tumor progression and metastasis. However, the role of L1 in gallbladder carcinoma (GBC) remains unclear. Here, we demonstrate that L1 is expressed in GBC cells and plays an important role in the growth, motility, invasiveness, and adhesiveness of GBC cells. Specific depletion or overexpression of L1 in the GBC cell lines JCRB1033 and SNU-308, respectively, was achieved by lentivirus-mediated transduction and expression of an L1 mRNA-specific short hairpin RNA or full-length human L1. Stable depletion of L1 led to a significant decrease in GBC cell proliferation, migration and invasion, as well as decreased intracellular signaling through AKT and FAK. Overexpression of L1 in GBC cells enhanced these cellular activities. In a GBC xenograft nude mouse model, suppression of L1 markedly reduced tumor growth and increased the survival of tumor-bearing mice whereas L1 overexpression stimulated tumorigenicity. Taken together, these results suggest that L1 plays a crucial role in GBC progression and may be a novel therapeutic target in GBC treatment.",
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Jung, J, Son, YS, Park, H, Jeon, SK, Lee, JW, Choi, SY, Kim, JM, Kwon, Y-G, Hong, HJ & Min, JK 2011, 'The cell adhesion molecule L1 promotes gallbladder carcinoma progression in vitro and in vivo', Oncology Reports, vol. 25, no. 4, pp. 945-952. https://doi.org/10.3892/or.2011.1181

The cell adhesion molecule L1 promotes gallbladder carcinoma progression in vitro and in vivo. / Jung, Juyeon; Son, Yeon Sung; Park, Hongryeol; Jeon, Seong Kook; Lee, Jung Whoi; Choi, Song Yi; Kim, Jin Man; Kwon, Young-Guen; Hong, Hyo Jeong; Min, Jeong Ki.

In: Oncology Reports, Vol. 25, No. 4, 01.04.2011, p. 945-952.

Research output: Contribution to journalArticle

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