The changes in the skin permeability barrier and epidermis of hairless mouse on oleic acid treatment

Ho Choi Eung Ho Choi, Ku Ahn Sung Ku Ahn, Hun Lee Seung Hun Lee

Research output: Contribution to journalArticle

Abstract

Background: The stratum corneum(SC) inhibits transepidermal water loss and makes a permeability barrier against foreign materials, so strategies to overcome relative impermeability of the SC is very important in transdermal drug delivery. This includes occlusion, hydration, chemical permeation enhancers, iontophoresis and sonophoresis. Oleic acid, which is one of the cis-unsaturated fatty acid and chemical permeation enhancers, increases the permeability of the lipophilic molecules and polar molecules through the SC. By spectrometry, calorimetry and the flux technique, the hypothesis that oleic acid does exist as a liquid within the SC lipids and enhances the transport of polar molecules across the SC by the formation of permeable interfacial defects within the SC lipid bilayers was suggested. Also, repeated application of oleic acid induces epidermal proliferation, hyperkeratosis and sebaceous gland hyperplasia; However the exact pathomechanism was not reported. Objective: We carried out some research to observe the mechanism by which oleic acid increases transdermal drug delivery and the effect to the skin permeability barrier and epidermis by repeated application. Methods: In the repeated treatment group, hairless mice(6 to 8 weeks) were treated with oleic acid once a day for 7 days unilaterally, and in the single treatment group, only one time. Transepidermal water loss(TEWL) was checked at 24hour after 1, 3 and 7 days of treatment and at 1 hour, 6 hours, 24 hours, 72 hours after single treatment. Biopsies were taken from treated and controlateral(control) sides immediately after the TEWL checks at each time for light microscopic(H and E stain) and electron microscopic studies. Results: In the repeated treatment group, TEWL was increased by day and epidermal proliferation and hyperkeratosis were also increased. In the single treatment group, TEWL was highly increased in the treated site at i hour after treatment and decreased with time. By electronmicroscope, we observed dilated lacunae, intercellular lipid structural abnormalities and loss of normal calcium gradient. Conclusion: The possible domains of the epidermis interacting with oleic acid as a penetration enhancer are the lacunae and lipid bilayer by EM. The suggested pathomechanism of the epidermal changes, epidermal proliferation and hyperkeratosis was increased DNA synthesis of epidermal cells by the loss of epidermal calcium gradient in chronic barrier impairment.

Original languageEnglish
Pages (from-to)702-711
Number of pages10
JournalKorean Journal of Dermatology
Volume35
Issue number4
Publication statusPublished - 1997 Jan 1

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Hairless Mouse
Oleic Acid
Epidermis
Cornea
Permeability
Skin
Water
Lipid Bilayers
Therapeutics
Calcium
Lipids
Sebaceous Glands
Iontophoresis
Calorimetry
Unsaturated Fatty Acids
Pharmaceutical Preparations
Hyperplasia
Spectrum Analysis
Coloring Agents
Electrons

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

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title = "The changes in the skin permeability barrier and epidermis of hairless mouse on oleic acid treatment",
abstract = "Background: The stratum corneum(SC) inhibits transepidermal water loss and makes a permeability barrier against foreign materials, so strategies to overcome relative impermeability of the SC is very important in transdermal drug delivery. This includes occlusion, hydration, chemical permeation enhancers, iontophoresis and sonophoresis. Oleic acid, which is one of the cis-unsaturated fatty acid and chemical permeation enhancers, increases the permeability of the lipophilic molecules and polar molecules through the SC. By spectrometry, calorimetry and the flux technique, the hypothesis that oleic acid does exist as a liquid within the SC lipids and enhances the transport of polar molecules across the SC by the formation of permeable interfacial defects within the SC lipid bilayers was suggested. Also, repeated application of oleic acid induces epidermal proliferation, hyperkeratosis and sebaceous gland hyperplasia; However the exact pathomechanism was not reported. Objective: We carried out some research to observe the mechanism by which oleic acid increases transdermal drug delivery and the effect to the skin permeability barrier and epidermis by repeated application. Methods: In the repeated treatment group, hairless mice(6 to 8 weeks) were treated with oleic acid once a day for 7 days unilaterally, and in the single treatment group, only one time. Transepidermal water loss(TEWL) was checked at 24hour after 1, 3 and 7 days of treatment and at 1 hour, 6 hours, 24 hours, 72 hours after single treatment. Biopsies were taken from treated and controlateral(control) sides immediately after the TEWL checks at each time for light microscopic(H and E stain) and electron microscopic studies. Results: In the repeated treatment group, TEWL was increased by day and epidermal proliferation and hyperkeratosis were also increased. In the single treatment group, TEWL was highly increased in the treated site at i hour after treatment and decreased with time. By electronmicroscope, we observed dilated lacunae, intercellular lipid structural abnormalities and loss of normal calcium gradient. Conclusion: The possible domains of the epidermis interacting with oleic acid as a penetration enhancer are the lacunae and lipid bilayer by EM. The suggested pathomechanism of the epidermal changes, epidermal proliferation and hyperkeratosis was increased DNA synthesis of epidermal cells by the loss of epidermal calcium gradient in chronic barrier impairment.",
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The changes in the skin permeability barrier and epidermis of hairless mouse on oleic acid treatment. / Eung Ho Choi, Ho Choi; Sung Ku Ahn, Ku Ahn; Seung Hun Lee, Hun Lee.

In: Korean Journal of Dermatology, Vol. 35, No. 4, 01.01.1997, p. 702-711.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The changes in the skin permeability barrier and epidermis of hairless mouse on oleic acid treatment

AU - Eung Ho Choi, Ho Choi

AU - Sung Ku Ahn, Ku Ahn

AU - Seung Hun Lee, Hun Lee

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Y1 - 1997/1/1

N2 - Background: The stratum corneum(SC) inhibits transepidermal water loss and makes a permeability barrier against foreign materials, so strategies to overcome relative impermeability of the SC is very important in transdermal drug delivery. This includes occlusion, hydration, chemical permeation enhancers, iontophoresis and sonophoresis. Oleic acid, which is one of the cis-unsaturated fatty acid and chemical permeation enhancers, increases the permeability of the lipophilic molecules and polar molecules through the SC. By spectrometry, calorimetry and the flux technique, the hypothesis that oleic acid does exist as a liquid within the SC lipids and enhances the transport of polar molecules across the SC by the formation of permeable interfacial defects within the SC lipid bilayers was suggested. Also, repeated application of oleic acid induces epidermal proliferation, hyperkeratosis and sebaceous gland hyperplasia; However the exact pathomechanism was not reported. Objective: We carried out some research to observe the mechanism by which oleic acid increases transdermal drug delivery and the effect to the skin permeability barrier and epidermis by repeated application. Methods: In the repeated treatment group, hairless mice(6 to 8 weeks) were treated with oleic acid once a day for 7 days unilaterally, and in the single treatment group, only one time. Transepidermal water loss(TEWL) was checked at 24hour after 1, 3 and 7 days of treatment and at 1 hour, 6 hours, 24 hours, 72 hours after single treatment. Biopsies were taken from treated and controlateral(control) sides immediately after the TEWL checks at each time for light microscopic(H and E stain) and electron microscopic studies. Results: In the repeated treatment group, TEWL was increased by day and epidermal proliferation and hyperkeratosis were also increased. In the single treatment group, TEWL was highly increased in the treated site at i hour after treatment and decreased with time. By electronmicroscope, we observed dilated lacunae, intercellular lipid structural abnormalities and loss of normal calcium gradient. Conclusion: The possible domains of the epidermis interacting with oleic acid as a penetration enhancer are the lacunae and lipid bilayer by EM. The suggested pathomechanism of the epidermal changes, epidermal proliferation and hyperkeratosis was increased DNA synthesis of epidermal cells by the loss of epidermal calcium gradient in chronic barrier impairment.

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