The changes of PCR ribotype and antimicrobial resistance of Clostridium difficile in a tertiary care hospital over 10 years

Jong Han Lee, Yangsoon Lee, Kyungwon Lee, Thomas V. Riley, Heejung Kim

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The aims of this study were to investigate any change in PCR ribotypes and to determine the antimicrobial resistance of common PCR ribotypes over a 10-year period in a tertiary care hospital. We conducted PCR ribotyping, antimicrobial susceptibility testing and DNA gyrase sequencing to identify changes in 1407 Clostridium difficile non-duplicated isolates obtained between 2000 and 2009. A total of 74 different ribotypes were found. The most prevalent ribotype was ribotype 001 (26.1 %). The prevalence of ribotype 017 was 17% and that of ribotype 014/020 was 9.6 %. Ribotyping showed that the prevalence of ribotype 001 decreased and the prevalence of ribotypes 017, 014/020 and 018 increased over the 10 years. Antimicrobial resistance rates in prevalent ribotypes were: clindamycin, 81 %; cefotetan, 19%; moxifloxacin, 42 %; imipenem, 8%; ciprofloxacin, 100% and erythromycin, 80 %. Ribotype 018 showed greater antimicrobial resistance than other ribotypes. All ribotype 018 strains showing moxifloxacin resistance had a substitution of a gyrA coding amino acid (Thr82 to Ile). This study will help the understanding of PCR ribotype trends and antimicrobial resistance of C. difficile in Korea.

Original languageEnglish
Pages (from-to)819-823
Number of pages5
JournalJournal of Medical Microbiology
Volume63
Issue numberPART 6
DOIs
Publication statusPublished - 2014 Jan 1

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Ribotyping
Clostridium difficile
Tertiary Healthcare
Tertiary Care Centers
Polymerase Chain Reaction
Cefotetan
DNA Gyrase
Clindamycin
Imipenem

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

Lee, Jong Han ; Lee, Yangsoon ; Lee, Kyungwon ; Riley, Thomas V. ; Kim, Heejung. / The changes of PCR ribotype and antimicrobial resistance of Clostridium difficile in a tertiary care hospital over 10 years. In: Journal of Medical Microbiology. 2014 ; Vol. 63, No. PART 6. pp. 819-823.
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The changes of PCR ribotype and antimicrobial resistance of Clostridium difficile in a tertiary care hospital over 10 years. / Lee, Jong Han; Lee, Yangsoon; Lee, Kyungwon; Riley, Thomas V.; Kim, Heejung.

In: Journal of Medical Microbiology, Vol. 63, No. PART 6, 01.01.2014, p. 819-823.

Research output: Contribution to journalArticle

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