The coat protein of turnip crinkle virus is involved in subviral RNA- mediated symptom modulation and accumulation

Qingzhong Kong, Jong Won Oh, Clifford D. Carpenter, Anne E. Simon

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Some satellite (sat-) and defective interfering (DI) RNAs associated with plant viruses intensify or ameliorate the symptoms of the virus. We recently demonstrated that the TCV coat protein (CP) is involved in symptom modulation by sat-RNA C. Two additional subviral RNAs have now been tested for effect of the CP on symptom modulation. DI RNA G, which normally intensifies the symptoms of TCV, is able to attenuate symptoms if the TCV CP is replaced with the CP of cardamine chlorotic fleck virus. DI RNA G had no effect on the symptoms of TCV with a single base alteration in the CP open reading frame, unlike sat-RNA C, which was able to ameliorate the symptoms of the mutant TCV. Using a hybrid sat-RNA constructed from sat-RNA C and TCV (which shares a similar 3'-end region with DI RNA G), the 3'-terminal 53 bases of sat-RNA C were found to be involved in symptom attenuation, which was directly correlated with the lack of detectable viral genomic RNA in whole plants. Sat-RNA D had no effect on the symptoms of mutant or wild-type TCV. The accumulation of TCV subviral RNAs in plants and protoplasts was also found to be strongly influenced by the presence or absence of the wild-type TCV CP.

Original languageEnglish
Pages (from-to)478-485
Number of pages8
JournalVirology
Volume238
Issue number2
DOIs
Publication statusPublished - 1997 Nov 24

Bibliographical note

Funding Information:
We thank H. Guan for providing sat-RNA C*. This work was supported by NSF Grants MCB-9315948 and MCB-9419303 awarded to A.E.S.

All Science Journal Classification (ASJC) codes

  • Virology

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