The comparison of sponges and PLGA scaffolds impregnated with DBP on growth behaviors of human intervertebral disc cells

Seon Kyoung Lee, Hee Kyung Hong, Su Jin Kim, Yong Ki Kim, Yi Seul Song, Yoon Ha, Dongwon Lee, Gilson Khang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We fabricated sponge and poly(lactide-co-glycolide) (PLGA) scaffolds impregnated demineralized bone particle (DBP) (DBP/PLGA) and investigated proper condition to proliferation and phenotype maintenance of intervertebral disc (IVD) cells by comparison between DBP/PLGA scaffold and DBP sponge. DBP/PLGA scaffolds were prepared by solvent casting/salt leaching. Human IVD cells were seeded in scaffolds of two types. Cell viability and proliferation according to scaffolds were analyzed by WST assay and SEM. RT-PCR was assessed to measure mRNA expression of aggrecan and type II collagen of human IVD cells. In WST assay results, cell viability in scaffolds impregnated DBP/PLGA scaffold were higher than DBP sponge. We could observe that disc cell mRNA expressed better in DBP/PLGA scaffold than DBP sponge. We concluded that the using of DBP/PLGA in terms of scaffold fabrication for bio-disc with human IVD cells is helpful growth of disc cells maintenance of phenotypes.

Original languageEnglish
Pages (from-to)398-404
Number of pages7
JournalPolymer (Korea)
Volume34
Issue number5
DOIs
Publication statusPublished - 2010 Sep

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)
  • Polymers and Plastics
  • Materials Chemistry

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