The conserved and divergent roles of carbonic anhydrases in the filamentous fungi Aspergillus fumigatus and Aspergillus nidulans

Kap Hoon Han, Yoon Hee Chun, Bárbara De Castro Pimentel Figueiredo, Frederico Marianetti Soriani, Marcela Savoldi, Agostinho Almeida, Fernando Rodrigues, Charlie Timothy Cairns, Elaine Bignell, Jaqueline Moisés Tobal, Maria Helena S. Goldman, Jong Hwan Kim, Yong Sun Bahn, Gustavo Henrique Goldman, Márcia Eliana Da Silva Ferreira

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17 Citations (Scopus)

Abstract

Carbon dioxide (CO2) and its hydration product bicarbonate (HCO3-) are essential molecules in various physiological processes of all living organisms. The reversible interconversion between CO2 and HCO3- is in equilibrium. This reaction is slow without catalyst, but can be rapidly facilitated by Zn 2+-metalloenzymes named carbonic anhydrases (CAs). To gain an insight into the function of multiple clades of fungal CA, we chose to investigate the filamentous fungi Aspergillus fumigatus and A. nidulans. We identified four and two CAs in A. fumigatus and A. nidulans, respectively, named cafA-D and canA-B. The cafA and cafB genes are constitutively, strongly expressed whereas cafC and cafD genes are weakly expressed but CO2-inducible. Heterologous expression of the A. fumigatus cafB, and A. nidulans canA and canB genes completely rescued the high CO2-requiring phenotype of a Saccharomyces cerevisiae Δnce103 mutant. Only the ΔcafA ΔcafB and ΔcanB deletion mutants were unable to grow at 0.033% CO2, of which growth defects can be restored by high CO2. Defects in the CAs can affect Aspergilli conidiation. Furthermore, A. fumigatus ΔcafA, ΔcafB, ΔcafC, ΔcafD and ΔcafA ΔcafB mutant strains are fully virulent in a low-dose murine infection.

Original languageEnglish
Pages (from-to)1372-1388
Number of pages17
JournalMolecular Microbiology
Volume75
Issue number6
DOIs
Publication statusPublished - 2010 Mar

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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    Han, K. H., Chun, Y. H., De Castro Pimentel Figueiredo, B., Soriani, F. M., Savoldi, M., Almeida, A., Rodrigues, F., Cairns, C. T., Bignell, E., Tobal, J. M., Goldman, M. H. S., Kim, J. H., Bahn, Y. S., Goldman, G. H., & Da Silva Ferreira, M. E. (2010). The conserved and divergent roles of carbonic anhydrases in the filamentous fungi Aspergillus fumigatus and Aspergillus nidulans. Molecular Microbiology, 75(6), 1372-1388. https://doi.org/10.1111/j.1365-2958.2010.07058.x