The conserved CA/TG motif at Mu termini: T specifies stable transpososome assembly

In suk Lee, Rasika M. Harshey

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The dinucleotide CA/TG found at the termini of transposable phage Mu occurs also at the termini of a large class of transposable elements, including HIV, all retroviruses and many retrotransposons. It was shown recently that mutations of this sequence block transpososome assembly, that A/T is more critical for activity than C/G, and that the hierarchy of reactivity of mutant termini follows closely the reported hierarchy of flexibility of their dinucleotide steps. In order to test the hypothesis that the terminal dinucleotide plays an essential structural role during "open termini" formation accompanying assembly, we have examined the activity of substrates carrying 100 different pairs of mismatched termini. Consistent with the flexibility hypothesis, we find that mismatched substrates are extremely efficient at assembly. A wild-type T residue on the bottom strand is essential for stable assembly, but the identity of the dinucleotide on the top strand is irrelevant for transposition chemistry. In addition, we have found a new rule for suppression of terminal defects by MuB protein, as well as a role for metal ions in DNA opening at the termini.

Original languageEnglish
Pages (from-to)261-275
Number of pages15
JournalJournal of Molecular Biology
Volume330
Issue number2
DOIs
Publication statusPublished - 2003 Jul 4

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Bacteriophage mu
Retroelements
DNA Transposable Elements
Retroviridae
Metals
HIV
Ions
Mutation
DNA
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

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The conserved CA/TG motif at Mu termini : T specifies stable transpososome assembly. / Lee, In suk; Harshey, Rasika M.

In: Journal of Molecular Biology, Vol. 330, No. 2, 04.07.2003, p. 261-275.

Research output: Contribution to journalArticle

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