The delivery strategy of paclitaxel nanostructured lipid carrier coated with platelet membrane

Ki Hyun Bang, Young Guk Na, Hyun Wook Huh, Sung Joo Hwang, Min Soo Kim, Minki Kim, Hong Ki Lee, Cheong Weon Cho

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Strategies for the development of anticancer drug delivery systems have undergone a dramatic transformation in the last few decades. Lipid-based drug delivery systems, such as a nanostructured lipid carrier (NLC), are one of the systems emerging to improve the outcomes of tumor treatments. However, NLC can act as an intruder and cause an immune response. To overcome this limitation, biomimicry technology was introduced to decorate the surface of the nanoparticles with various cell membrane proteins. Here, we designed paclitaxel (PT)-loaded nanostructured lipid carrier (PT-NLC) with platelet (PLT) membrane protein because PLT is involved with angiogenesis and interaction of circulating tumor cells. After PLT was isolated from blood using the gravity-gradient method and it was used for coating PT-NLC. Spherical PT-NLC and platelet membrane coated PT-NLC (P-PT-NLC) were successfully fabricated with high encapsulation efficiency (EE) (99.98%) and small particle size (less than 200 nm). The successful coating of PT-NLC with a PLT membrane was confirmed by the identification of CD41 based on transmission electron microscopy (TEM), western blot assay and enzyme-linked immunosorbent assay (ELISA) data. Moreover, the stronger affinity of P-PT-NLC than that of PT-NLC toward tumor cells was observed. In vitro cell study, the PLT coated nanoparticles successfully displayed the anti-tumor effect to SK-OV-3 cells. In summary, the biomimicry carrier system P-PT-NLC has an affinity and targeting ability for tumor cells.

Original languageEnglish
Article number807
JournalCancers
Volume11
Issue number6
DOIs
Publication statusPublished - 2019

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'The delivery strategy of paclitaxel nanostructured lipid carrier coated with platelet membrane'. Together they form a unique fingerprint.

  • Cite this

    Bang, K. H., Na, Y. G., Huh, H. W., Hwang, S. J., Kim, M. S., Kim, M., Lee, H. K., & Cho, C. W. (2019). The delivery strategy of paclitaxel nanostructured lipid carrier coated with platelet membrane. Cancers, 11(6), [807]. https://doi.org/10.3390/cancers11060807