The diagnostic approach to fine-needle aspiration of malignant lymphoma

Using cytomorphology and immunocytochemistry with cell transfer method

Eun Ji Oh, Soon Won Hong, Hyeong Jae Jeong, SunOch Yoon

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Fine-needle aspiration (FNA) cytology is generally considered to be the screening tool for lymphoproliferative lesions. The differential and decisive diagnosis, however, of malignant lymphoma from benign reactive hyperplasia by FNA cytology is sometimes challenging. The diagnostic features compatible with lymphoma as opposed to reactive hyperplasia in FNA cytology were investigated with 31 cases of lymphoma and 31 cases of reactive hyperplasia, and immunocytochemistry with cell transfer method was additionally applied to FNA cytology. The predominance of large lymphocytes, the clustering of large lymphocytes, the presence of markedly large and/or highly pleomorphic cells, the presence of apoptotic and/or necrotic cell debris were considered characteristics of lymphomas, whereas the predominance of small lymphocytes and the presence of histiocytes were considered characteristics of reactive hyperplasia. Using these cytomorphologic characteristics, the diagnostic accuracy for malignant lymphoma in FNA cytology had a sensitivity of 80.6% and a specificity of 100%. By cell transfer method, one of Papanicolaou-stained slides could be used in immunocytochemistry for several markers. Using such methods, sensitivity of FNA cytology for lymphoma was upgraded to 100%, and decisive diagnoses of diffuse large B-cell lymphoma, Burkitt lymphoma, low grade B-cell lymphoma, T- or NK-cell non-Hodgkin lymphoma (NHL), or Hodgkin lymphoma was possible. Differential diagnosis of malignant lymphoma from reactive hyperplasia, and decisive diagnoses of high, and low grade B-cell NHL, T- or NK-cell NHL, and HL could be possible by FNA cytology with cytomorphology in conjunction with immunocytochemistry using cell transfer method.

Original languageEnglish
Pages (from-to)671-679
Number of pages9
JournalDiagnostic Cytopathology
Volume42
Issue number8
DOIs
Publication statusPublished - 2014 Jan 1

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Fine Needle Biopsy
Cell Biology
Lymphoma
Immunohistochemistry
Hyperplasia
Non-Hodgkin's Lymphoma
B-Cell Lymphoma
Lymphocytes
Natural Killer Cells
Differential Diagnosis
Histiocytes
Burkitt Lymphoma
Lymphoma, Large B-Cell, Diffuse
Hodgkin Disease
Cluster Analysis

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology

Cite this

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title = "The diagnostic approach to fine-needle aspiration of malignant lymphoma: Using cytomorphology and immunocytochemistry with cell transfer method",
abstract = "Fine-needle aspiration (FNA) cytology is generally considered to be the screening tool for lymphoproliferative lesions. The differential and decisive diagnosis, however, of malignant lymphoma from benign reactive hyperplasia by FNA cytology is sometimes challenging. The diagnostic features compatible with lymphoma as opposed to reactive hyperplasia in FNA cytology were investigated with 31 cases of lymphoma and 31 cases of reactive hyperplasia, and immunocytochemistry with cell transfer method was additionally applied to FNA cytology. The predominance of large lymphocytes, the clustering of large lymphocytes, the presence of markedly large and/or highly pleomorphic cells, the presence of apoptotic and/or necrotic cell debris were considered characteristics of lymphomas, whereas the predominance of small lymphocytes and the presence of histiocytes were considered characteristics of reactive hyperplasia. Using these cytomorphologic characteristics, the diagnostic accuracy for malignant lymphoma in FNA cytology had a sensitivity of 80.6{\%} and a specificity of 100{\%}. By cell transfer method, one of Papanicolaou-stained slides could be used in immunocytochemistry for several markers. Using such methods, sensitivity of FNA cytology for lymphoma was upgraded to 100{\%}, and decisive diagnoses of diffuse large B-cell lymphoma, Burkitt lymphoma, low grade B-cell lymphoma, T- or NK-cell non-Hodgkin lymphoma (NHL), or Hodgkin lymphoma was possible. Differential diagnosis of malignant lymphoma from reactive hyperplasia, and decisive diagnoses of high, and low grade B-cell NHL, T- or NK-cell NHL, and HL could be possible by FNA cytology with cytomorphology in conjunction with immunocytochemistry using cell transfer method.",
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The diagnostic approach to fine-needle aspiration of malignant lymphoma : Using cytomorphology and immunocytochemistry with cell transfer method. / Oh, Eun Ji; Hong, Soon Won; Jeong, Hyeong Jae; Yoon, SunOch.

In: Diagnostic Cytopathology, Vol. 42, No. 8, 01.01.2014, p. 671-679.

Research output: Contribution to journalArticle

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