The divIVA minicell locus of Bacillus subtilis

JeongHeon Cha, George C. Stewart

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

The Bacillus subtilis divIVA1 mutation causes misplacement of the septum during cell division, resulting in the formation of small, circular, anucleate minicells. This study reports the cloning and sequence analysis of 2.4 kb of the B. subtilis chromosome including the divIVA locus. Three open reading flames were identified: or, whose function is unknown; divIVA; and isoleucyl tRNA synthetase (ileS). We identified the point mutation in the divIVA1 mutant allele. Inactivation of divIVA produces a minicell phenotype, whereas overproduction of DivIVA results in a filamentation phenotype. Mutants with mutations at both of the minicell loci of B. subtilis, divIVA and divIVB, possess a minicell phenotype identical to that of the DivIVB- mutant. The DIvIVA-mutants, but not the DivIVB- mutants, show a decrease in sporulation efficiency and a delay in the kinetics of endospore formation. The data support a model in which divIVA encodes the topological specificity subunit of the minCD system. The model suggests that DivIVA acts as u pilot protein, directing minCD to the polar septation sites. DivIVA also appears to be the interface between a sporulation component and MinCD, freeing up the polar septation sites for use during the asymmetric septation event of the sporulation process.

Original languageEnglish
Pages (from-to)1671-1683
Number of pages13
JournalJournal of Bacteriology
Volume179
Issue number5
DOIs
Publication statusPublished - 1997 Jan 1

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Bacillus subtilis
Phenotype
Isoleucine-tRNA Ligase
RNA, Transfer, Ile
Mutation
Point Mutation
Cell Division
Sequence Analysis
Organism Cloning
Reading
Chromosomes
Alleles
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Cha, JeongHeon ; Stewart, George C. / The divIVA minicell locus of Bacillus subtilis. In: Journal of Bacteriology. 1997 ; Vol. 179, No. 5. pp. 1671-1683.
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The divIVA minicell locus of Bacillus subtilis. / Cha, JeongHeon; Stewart, George C.

In: Journal of Bacteriology, Vol. 179, No. 5, 01.01.1997, p. 1671-1683.

Research output: Contribution to journalArticle

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