The E3 ubiquitin ligase MARCH2 regulates ERGIC3-dependent trafficking of secretory proteins

Wonjin Yoo, Eun Bee Cho, Sungjoo Kim, Jong Bok Yoon

Research output: Contribution to journalArticle

Abstract

The E3 ubiquitin ligase membrane-associated ring-CH–type finger 2 (MARCH2) is known to be involved in intracellular vesicular trafficking, but its role in the early secretory pathway between the endoplasmic reticulum (ER) and Golgi compartments is largely unknown. Human ER–Golgi intermediate compartment protein 2 (ERGIC2) and ERGIC3 are orthologs of Erv41 and Erv46 in yeast, proteins that form a heteromeric complex, cycle between the ER and Golgi, and function as cargo receptors in both anterograde and retrograde protein trafficking. Here, we report that MARCH2 directs ubiquitination and subsequent degradation of ERGIC3 and that MARCH2 depletion increases endogenous ERGIC3 levels. We provide evidence that the lysine residues at positions 6 and 8 of ERGIC3 are the major sites of MARCH2-mediated ubiquitination. Of note, MARCH2 did not significantly decrease the levels of an ERGIC3 variant with lysine-to-arginine substitutions at residues 6 and 8. We also show that ERGIC3 binds to itself or to ERGIC2, whereas ERGIC2 is unable to interact with itself. Our results indicate that 1-antitrypsin and haptoglobin are likely to be cargo proteins of ERGIC3. We further observed that 1-antitrypsin and haptoglobin specifically bind to ERGIC3 and that ERGIC3 depletion decreases their secretion. Moreover, MARCH2 reduced secretion of 1-antitrypsin and haptoglobin, and coexpression of the ubiquitination-resistant ERGIC3 variant largely restored their secretion, suggesting that MARCH2-mediated ERGIC3 ubiquitination is the major cause of the decrease in trafficking of ERGIC3-binding secretory proteins. Our findings provide detailed insights into the regulation of the early secretory pathway by MARCH2 and into ERGIC3 function.

Original languageEnglish
Pages (from-to)10900-10912
Number of pages13
JournalJournal of Biological Chemistry
Volume294
Issue number28
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

Ubiquitin-Protein Ligases
Protein Transport
Fingers
Membranes
Ubiquitination
Haptoglobins
Proteins
Secretory Pathway
Endoplasmic Reticulum
Lysine
Fungal Proteins
Arginine
Carrier Proteins
Substitution reactions
Degradation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Yoo, Wonjin ; Cho, Eun Bee ; Kim, Sungjoo ; Yoon, Jong Bok. / The E3 ubiquitin ligase MARCH2 regulates ERGIC3-dependent trafficking of secretory proteins. In: Journal of Biological Chemistry. 2019 ; Vol. 294, No. 28. pp. 10900-10912.
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abstract = "The E3 ubiquitin ligase membrane-associated ring-CH–type finger 2 (MARCH2) is known to be involved in intracellular vesicular trafficking, but its role in the early secretory pathway between the endoplasmic reticulum (ER) and Golgi compartments is largely unknown. Human ER–Golgi intermediate compartment protein 2 (ERGIC2) and ERGIC3 are orthologs of Erv41 and Erv46 in yeast, proteins that form a heteromeric complex, cycle between the ER and Golgi, and function as cargo receptors in both anterograde and retrograde protein trafficking. Here, we report that MARCH2 directs ubiquitination and subsequent degradation of ERGIC3 and that MARCH2 depletion increases endogenous ERGIC3 levels. We provide evidence that the lysine residues at positions 6 and 8 of ERGIC3 are the major sites of MARCH2-mediated ubiquitination. Of note, MARCH2 did not significantly decrease the levels of an ERGIC3 variant with lysine-to-arginine substitutions at residues 6 and 8. We also show that ERGIC3 binds to itself or to ERGIC2, whereas ERGIC2 is unable to interact with itself. Our results indicate that 1-antitrypsin and haptoglobin are likely to be cargo proteins of ERGIC3. We further observed that 1-antitrypsin and haptoglobin specifically bind to ERGIC3 and that ERGIC3 depletion decreases their secretion. Moreover, MARCH2 reduced secretion of 1-antitrypsin and haptoglobin, and coexpression of the ubiquitination-resistant ERGIC3 variant largely restored their secretion, suggesting that MARCH2-mediated ERGIC3 ubiquitination is the major cause of the decrease in trafficking of ERGIC3-binding secretory proteins. Our findings provide detailed insights into the regulation of the early secretory pathway by MARCH2 and into ERGIC3 function.",
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The E3 ubiquitin ligase MARCH2 regulates ERGIC3-dependent trafficking of secretory proteins. / Yoo, Wonjin; Cho, Eun Bee; Kim, Sungjoo; Yoon, Jong Bok.

In: Journal of Biological Chemistry, Vol. 294, No. 28, 01.01.2019, p. 10900-10912.

Research output: Contribution to journalArticle

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