The E6 and E7 genes of human papilloma virus-type 16 protect primary astrocyte cultures from injury

Jongeun Lee, Charlotte Y. Kim, Amato J. Giaccia, Rona G. Giffard

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Many oncogenes are implicated in the regulation of apoptosis as well as in control of the cell cycle, and several have been shown to protect cells from injury. We tested whether the expression of human papillomavirus type 16 genes E6 and E7 could protect primary astrocytes from injury. Retroviral vectors were used to express E6, E7, or E6E7 in primary murine astrocyte cultures. Astrocytes expressing E6E7 suffered less than half the injury seen in controls after exposure to 400 μM H2O2. When we compared astrocytes expressing only E6 or E7, cells expressing E7 alone were protected to a greater extent and from more severe injury than those expressing E6. E6E7, E6 and E7 all provided protection from 30 h glucose deprivation, but again E7 provided the best protection, reducing injury to less than a third of that seen in controls. Expression of E7 alone decreases vulnerability to both hydrogen peroxide and glucose deprivation injury while E6 alone primarily decreases glucose deprivation injury. These results demonstrate that expression of human papillomavirus oncogenes can reduce the vulnerability of astrocytes to oxidative stress and nutrient deprivation.

Original languageEnglish
Pages (from-to)10-16
Number of pages7
JournalBrain Research
Volume795
Issue number1-2
DOIs
Publication statusPublished - 1998 Jun 8

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Papillomaviridae
Astrocytes
Wounds and Injuries
Genes
Oncogenes
Glucose
Human papillomavirus 16
Cell Cycle Checkpoints
Hydrogen Peroxide
Oxidative Stress
Apoptosis

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Lee, Jongeun ; Kim, Charlotte Y. ; Giaccia, Amato J. ; Giffard, Rona G. / The E6 and E7 genes of human papilloma virus-type 16 protect primary astrocyte cultures from injury. In: Brain Research. 1998 ; Vol. 795, No. 1-2. pp. 10-16.
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The E6 and E7 genes of human papilloma virus-type 16 protect primary astrocyte cultures from injury. / Lee, Jongeun; Kim, Charlotte Y.; Giaccia, Amato J.; Giffard, Rona G.

In: Brain Research, Vol. 795, No. 1-2, 08.06.1998, p. 10-16.

Research output: Contribution to journalArticle

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