The effect of ASK1 on vascular permeability and edema formation in cerebral ischemia

Juhyun Song, So Yeong Cheon, Won Taek Lee, Kyung Ah Park, Jongeun Lee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Apoptosis signal-regulating kinase-1 (ASK1) is the mitogen-activated protein kinase kinase kinase (MAPKKK) and participates in the various central nervous system (CNS) signaling pathways. In cerebral ischemia, vascular permeability in the brain is an important issue because regulation failure of it results in edema formation and blood-brain barrier (BBB) disruption. To determine the role of ASK1 on vascular permeability and edema formation following cerebral ischemia, we first investigated ASK1-related gene expression using microarray analyses of ischemic brain tissue. We then measured protein levels of ASK1 and vascular endothelial growth factor (VEGF) in brain endothelial cells after hypoxia injury. We also examined protein expression of ASK1 and VEGF, edema formation, and morphological alteration through cresyl violet staining in ischemic brain tissue using ASK1-small interference RNA (ASK1-siRNA). Finally, immunohistochemistry was performed to examine VEGF and aquaporin-1 (AQP-1) expression in ischemic brain injury. Based on our findings, we propose that ASK1 is a regulating factor of vascular permeability and edema formation in cerebral ischemia.

Original languageEnglish
Pages (from-to)143-155
Number of pages13
JournalBrain Research
Volume1595
DOIs
Publication statusPublished - 2015 Jan 1

Fingerprint

MAP Kinase Kinase Kinase 5
Capillary Permeability
Brain Ischemia
Edema
Vascular Endothelial Growth Factor A
Brain
Aquaporin 1
MAP Kinase Kinase Kinases
Cell Hypoxia
Microarray Analysis
RNA Interference
Blood-Brain Barrier
Brain Injuries
Proteins
Central Nervous System
Endothelial Cells
Immunohistochemistry
Staining and Labeling
Gene Expression

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Song, Juhyun ; Cheon, So Yeong ; Lee, Won Taek ; Park, Kyung Ah ; Lee, Jongeun. / The effect of ASK1 on vascular permeability and edema formation in cerebral ischemia. In: Brain Research. 2015 ; Vol. 1595. pp. 143-155.
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The effect of ASK1 on vascular permeability and edema formation in cerebral ischemia. / Song, Juhyun; Cheon, So Yeong; Lee, Won Taek; Park, Kyung Ah; Lee, Jongeun.

In: Brain Research, Vol. 1595, 01.01.2015, p. 143-155.

Research output: Contribution to journalArticle

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