The effect of bit number and sampling rate of a digitizer on least-square multi exponential decay analysis in fluorescence lifetime imaging

Won Sang Hwang, Dong Eun Kim, Jun Woo Kim, Youn Young Ji, Byung Hwy So, Dug Young Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have simulated the effects of the number of bits and the sampling rate of a digitizer on the performance of lifetime measurements. We found that the number of bits of a digitizer is important to obtain certain accuracy in lifetime measurement. There exists a certain critical sampling frequency of a digitizer required to separate a certain lifetime differences in a double exponentially decaying intensity profile. We did these simulations by using Monte Carlo simulations with least-square curve fitting algorithms.

Original languageEnglish
Title of host publicationHigh-Speed Biomedical Imaging and Spectroscopy
Subtitle of host publicationToward Big Data Instrumentation and Management II
EditorsKevin K. Tsia, Keisuke Goda
PublisherSPIE
ISBN (Electronic)9781510605930
DOIs
Publication statusPublished - 2017 Jan 1
EventHigh-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II 2017 - San Francisco, United States
Duration: 2017 Jan 302017 Feb 1

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10076
ISSN (Print)1605-7422

Other

OtherHigh-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II 2017
CountryUnited States
CitySan Francisco
Period17/1/3017/2/1

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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    Hwang, W. S., Kim, D. E., Kim, J. W., Ji, Y. Y., So, B. H., & Kim, D. Y. (2017). The effect of bit number and sampling rate of a digitizer on least-square multi exponential decay analysis in fluorescence lifetime imaging. In K. K. Tsia, & K. Goda (Eds.), High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II [100761H] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10076). SPIE. https://doi.org/10.1117/12.2250730