The effect of bit number and sampling rate of a digitizer on least-square multi exponential decay analysis in fluorescence lifetime imaging

Won Sang Hwang; Dong Eun Kim; Jun Woo Kim; Youn Young Ji; Byung Hwy So; Dug Young Kim

doi:10.1117/12.2250730

The effect of bit number and sampling rate of a digitizer on least-square multi exponential decay analysis in fluorescence lifetime imaging

Won Sang Hwang, Dong Eun Kim, Jun Woo Kim, Youn Young Ji, Byung Hwy So, Dug Young Kim

Department of Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We have simulated the effects of the number of bits and the sampling rate of a digitizer on the performance of lifetime measurements. We found that the number of bits of a digitizer is important to obtain certain accuracy in lifetime measurement. There exists a certain critical sampling frequency of a digitizer required to separate a certain lifetime differences in a double exponentially decaying intensity profile. We did these simulations by using Monte Carlo simulations with least-square curve fitting algorithms.

Original language	English
Title of host publication	High-Speed Biomedical Imaging and Spectroscopy
Subtitle of host publication	Toward Big Data Instrumentation and Management II
Editors	Kevin K. Tsia, Keisuke Goda
Publisher	SPIE
ISBN (Electronic)	9781510605930
DOIs	https://doi.org/10.1117/12.2250730
Publication status	Published - 2017
Event	High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II 2017 - San Francisco, United States Duration: 2017 Jan 30 → 2017 Feb 1

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10076
ISSN (Print)	1605-7422

Other

Other	High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II 2017
Country/Territory	United States
City	San Francisco
Period	17/1/30 → 17/2/1

Bibliographical note

Publisher Copyright:
Copyright © 2017 SPIE.

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2250730

Cite this

Hwang, W. S., Kim, D. E., Kim, J. W., Ji, Y. Y., So, B. H., & Kim, D. Y. (2017). The effect of bit number and sampling rate of a digitizer on least-square multi exponential decay analysis in fluorescence lifetime imaging. In K. K. Tsia, & K. Goda (Eds.), High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II [100761H] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10076). SPIE. https://doi.org/10.1117/12.2250730

Hwang, Won Sang ; Kim, Dong Eun ; Kim, Jun Woo et al. / The effect of bit number and sampling rate of a digitizer on least-square multi exponential decay analysis in fluorescence lifetime imaging. High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II. editor / Kevin K. Tsia ; Keisuke Goda. SPIE, 2017. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

@inproceedings{05940d30c5e9459e9d655d7ce161fcd7,

title = "The effect of bit number and sampling rate of a digitizer on least-square multi exponential decay analysis in fluorescence lifetime imaging",

abstract = "We have simulated the effects of the number of bits and the sampling rate of a digitizer on the performance of lifetime measurements. We found that the number of bits of a digitizer is important to obtain certain accuracy in lifetime measurement. There exists a certain critical sampling frequency of a digitizer required to separate a certain lifetime differences in a double exponentially decaying intensity profile. We did these simulations by using Monte Carlo simulations with least-square curve fitting algorithms.",

author = "Hwang, {Won Sang} and Kim, {Dong Eun} and Kim, {Jun Woo} and Ji, {Youn Young} and So, {Byung Hwy} and Kim, {Dug Young}",

note = "Publisher Copyright: Copyright {\textcopyright} 2017 SPIE.; High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II 2017 ; Conference date: 30-01-2017 Through 01-02-2017",

year = "2017",

doi = "10.1117/12.2250730",

language = "English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

editor = "Tsia, {Kevin K.} and Keisuke Goda",

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address = "United States",

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Hwang, WS, Kim, DE, Kim, JW, Ji, YY, So, BH & Kim, DY 2017, The effect of bit number and sampling rate of a digitizer on least-square multi exponential decay analysis in fluorescence lifetime imaging. in KK Tsia & K Goda (eds), High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II., 100761H, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10076, SPIE, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II 2017, San Francisco, United States, 17/1/30. https://doi.org/10.1117/12.2250730

The effect of bit number and sampling rate of a digitizer on least-square multi exponential decay analysis in fluorescence lifetime imaging. / Hwang, Won Sang; Kim, Dong Eun; Kim, Jun Woo et al.

High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II. ed. / Kevin K. Tsia; Keisuke Goda. SPIE, 2017. 100761H (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10076).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AU - So, Byung Hwy

AU - Kim, Dug Young

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M3 - Conference contribution

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BT - High-Speed Biomedical Imaging and Spectroscopy

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Hwang WS, Kim DE, Kim JW, Ji YY, So BH, Kim DY. The effect of bit number and sampling rate of a digitizer on least-square multi exponential decay analysis in fluorescence lifetime imaging. In Tsia KK, Goda K, editors, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II. SPIE. 2017. 100761H. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2250730

The effect of bit number and sampling rate of a digitizer on least-square multi exponential decay analysis in fluorescence lifetime imaging

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