The effect of cyclic strain on embryonic stem cell-derived cardiomyocytes

So Jung Gwak, Suk Ho Bhang, Il Kwon Kim, Sang Soo Kim, Seung-Woo Cho, Oju Jeon, Kyung Jong Yoo, Andrew J. Putnam, Byung Soo Kim

Research output: Contribution to journalArticle

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Abstract

Cardiomyocytes in the body are subjected to cyclic mechanical strain induced by the rhythmic heart beating. In this study, we tested the hypothesis that cyclic strain promotes cardiomyogenesis of embryonic stem cell-derived cardiomyocytes (ESCs). ESCs cultured on elastic polymer [poly(lactide-co-caprolactone), PLCL] scaffolds subjected to cyclic strain in vitro displayed elevated cardiac gene expression compared to unstrained controls. Six weeks after implantation into infarcted rat myocardium, the elastic cardiac patches (ESC-seeded PLCL scaffolds) showed reduced fibrotic tissue formation, likely due to a combination of lower apoptotic activity, higher vascular endothelial growth factor (VEGF) expression, and more extensive angiogenesis in the strained versus unstrained control [ESC-seeded, non-elastic poly(lactide-co-glycolide) scaffolds] patches. Importantly, cardiac gene expression was upregulated in the elastic patches compared to control, with evidence for cardiomyocyte-specific microstructures including myofibrillar bundles and Z-lines. This study shows that the use of an elastic polymer scaffold designed to permit mechanical strain transduction as a cell transplantation vehicle significantly increases cardiomyogenesis of the implanted ESCs.

Original languageEnglish
Pages (from-to)844-856
Number of pages13
JournalBiomaterials
Volume29
Issue number7
DOIs
Publication statusPublished - 2008 Mar 1

Fingerprint

Embryonic Stem Cells
Stem cells
Cardiac Myocytes
Scaffolds
Gene expression
Polymers
Polyglactin 910
Gene Expression
Vascular Endothelial Growth Factor A
Rats
Cell Transplantation
Tissue
Microstructure
Myocardium

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Gwak, S. J., Bhang, S. H., Kim, I. K., Kim, S. S., Cho, S-W., Jeon, O., ... Kim, B. S. (2008). The effect of cyclic strain on embryonic stem cell-derived cardiomyocytes. Biomaterials, 29(7), 844-856. https://doi.org/10.1016/j.biomaterials.2007.10.050
Gwak, So Jung ; Bhang, Suk Ho ; Kim, Il Kwon ; Kim, Sang Soo ; Cho, Seung-Woo ; Jeon, Oju ; Yoo, Kyung Jong ; Putnam, Andrew J. ; Kim, Byung Soo. / The effect of cyclic strain on embryonic stem cell-derived cardiomyocytes. In: Biomaterials. 2008 ; Vol. 29, No. 7. pp. 844-856.
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Gwak, SJ, Bhang, SH, Kim, IK, Kim, SS, Cho, S-W, Jeon, O, Yoo, KJ, Putnam, AJ & Kim, BS 2008, 'The effect of cyclic strain on embryonic stem cell-derived cardiomyocytes', Biomaterials, vol. 29, no. 7, pp. 844-856. https://doi.org/10.1016/j.biomaterials.2007.10.050

The effect of cyclic strain on embryonic stem cell-derived cardiomyocytes. / Gwak, So Jung; Bhang, Suk Ho; Kim, Il Kwon; Kim, Sang Soo; Cho, Seung-Woo; Jeon, Oju; Yoo, Kyung Jong; Putnam, Andrew J.; Kim, Byung Soo.

In: Biomaterials, Vol. 29, No. 7, 01.03.2008, p. 844-856.

Research output: Contribution to journalArticle

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