The effect of cyclosporin A on the growth of human glioma cell lines

Jhin Soo Pyen; Soo Kie Kim; Sun Ju Choi; Yoon Sun Park; Hyun Chul Cho; Young Pyo Han

doi:10.1007/BF02976205

The effect of cyclosporin A on the growth of human glioma cell lines

Jhin Soo Pyen, Soo Kie Kim, Sun Ju Choi, Yoon Sun Park, Hyun Chul Cho, Young Pyo Han

Microbiology

Research output: Contribution to journal › Article

Abstract

Cyclosporin A, an potent immunosuppressant, has been known to be one of the modulators of drug resistance as well as a cytostatic drug. Despite many attempts to basic or clinical application of cyclosporin A, there are few reports on the inhibition of brain tumor cells. In the present experiment, the possibility of cyclosporin A as synergic adjuvant was investigated by MTT assay, [³H] thymidine uptake and through flowcytometric anaysis. Sole treatment of cyclosporin A on the CRT and CH235-MG glioma cell line revealed dose dependent cytotoxicity within a range of tested dose. Combined treatment of cyclosporin A with ACNU, BCNU and hydroxyurea on various glioma cancer cell line led to a significant synergistic cytotoxicity as well as inhibition of DNA synthesis with dose-dependency. In addition, cyclosporin A alone or combined treatment caused discernible changes of cell cycle in the tested cells. These data provide that cyclosporin A could potentiate the effect of nitrosourea compounds in vitro on human glioma cells.

Original language	English
Pages (from-to)	379-383
Number of pages	5
Journal	Archives of pharmacal research
Volume	20
Issue number	4
DOIs	https://doi.org/10.1007/BF02976205
Publication status	Published - 1997 Aug

Bibliographical note

Funding Information:
This work was supported by a Yonsei University Faculty Research Grovist for 1995.

All Science Journal Classification (ASJC) codes

Molecular Medicine
Drug Discovery
Organic Chemistry

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title = "The effect of cyclosporin A on the growth of human glioma cell lines",

abstract = "Cyclosporin A, an potent immunosuppressant, has been known to be one of the modulators of drug resistance as well as a cytostatic drug. Despite many attempts to basic or clinical application of cyclosporin A, there are few reports on the inhibition of brain tumor cells. In the present experiment, the possibility of cyclosporin A as synergic adjuvant was investigated by MTT assay, [3H] thymidine uptake and through flowcytometric anaysis. Sole treatment of cyclosporin A on the CRT and CH235-MG glioma cell line revealed dose dependent cytotoxicity within a range of tested dose. Combined treatment of cyclosporin A with ACNU, BCNU and hydroxyurea on various glioma cancer cell line led to a significant synergistic cytotoxicity as well as inhibition of DNA synthesis with dose-dependency. In addition, cyclosporin A alone or combined treatment caused discernible changes of cell cycle in the tested cells. These data provide that cyclosporin A could potentiate the effect of nitrosourea compounds in vitro on human glioma cells.",

author = "Pyen, {Jhin Soo} and Kim, {Soo Kie} and Choi, {Sun Ju} and Park, {Yoon Sun} and Cho, {Hyun Chul} and Han, {Young Pyo}",

note = "Funding Information: This work was supported by a Yonsei University Faculty Research Grovist for 1995.",

year = "1997",

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doi = "10.1007/BF02976205",

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AU - Pyen, Jhin Soo

AU - Kim, Soo Kie

AU - Choi, Sun Ju

AU - Park, Yoon Sun

AU - Cho, Hyun Chul

AU - Han, Young Pyo

N1 - Funding Information: This work was supported by a Yonsei University Faculty Research Grovist for 1995.

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Y1 - 1997/8

N2 - Cyclosporin A, an potent immunosuppressant, has been known to be one of the modulators of drug resistance as well as a cytostatic drug. Despite many attempts to basic or clinical application of cyclosporin A, there are few reports on the inhibition of brain tumor cells. In the present experiment, the possibility of cyclosporin A as synergic adjuvant was investigated by MTT assay, [3H] thymidine uptake and through flowcytometric anaysis. Sole treatment of cyclosporin A on the CRT and CH235-MG glioma cell line revealed dose dependent cytotoxicity within a range of tested dose. Combined treatment of cyclosporin A with ACNU, BCNU and hydroxyurea on various glioma cancer cell line led to a significant synergistic cytotoxicity as well as inhibition of DNA synthesis with dose-dependency. In addition, cyclosporin A alone or combined treatment caused discernible changes of cell cycle in the tested cells. These data provide that cyclosporin A could potentiate the effect of nitrosourea compounds in vitro on human glioma cells.

AB - Cyclosporin A, an potent immunosuppressant, has been known to be one of the modulators of drug resistance as well as a cytostatic drug. Despite many attempts to basic or clinical application of cyclosporin A, there are few reports on the inhibition of brain tumor cells. In the present experiment, the possibility of cyclosporin A as synergic adjuvant was investigated by MTT assay, [3H] thymidine uptake and through flowcytometric anaysis. Sole treatment of cyclosporin A on the CRT and CH235-MG glioma cell line revealed dose dependent cytotoxicity within a range of tested dose. Combined treatment of cyclosporin A with ACNU, BCNU and hydroxyurea on various glioma cancer cell line led to a significant synergistic cytotoxicity as well as inhibition of DNA synthesis with dose-dependency. In addition, cyclosporin A alone or combined treatment caused discernible changes of cell cycle in the tested cells. These data provide that cyclosporin A could potentiate the effect of nitrosourea compounds in vitro on human glioma cells.

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