The effect of cyclosporin A on the growth of human glioma cell lines

Jhin Soo Pyen, Soo Kie Kim, Sun Ju Choi, Yoon Sun Park, Hyun Chul Cho, Young Pyo Han

Research output: Contribution to journalArticle

Abstract

Cyclosporin A, an potent immunosuppressant, has been known to be one of the modulators of drug resistance as well as a cytostatic drug. Despite many attempts to basic or clinical application of cyclosporin A, there are few reports on the inhibition of brain tumor cells. In the present experiment, the possibility of cyclosporin A as synergic adjuvant was investigated by MTT assay, [3H] thymidine uptake and through flowcytometric anaysis. Sole treatment of cyclosporin A on the CRT and CH235-MG glioma cell line revealed dose dependent cytotoxicity within a range of tested dose. Combined treatment of cyclosporin A with ACNU, BCNU and hydroxyurea on various glioma cancer cell line led to a significant synergistic cytotoxicity as well as inhibition of DNA synthesis with dose-dependency. In addition, cyclosporin A alone or combined treatment caused discernible changes of cell cycle in the tested cells. These data provide that cyclosporin A could potentiate the effect of nitrosourea compounds in vitro on human glioma cells.

Original languageEnglish
Pages (from-to)379-383
Number of pages5
JournalArchives of pharmacal research
Volume20
Issue number4
DOIs
Publication statusPublished - 1997 Aug

Bibliographical note

Funding Information:
This work was supported by a Yonsei University Faculty Research Grovist for 1995.

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Drug Discovery
  • Organic Chemistry

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