The effect of DA-9701 in Opioid-induced bowel dysfunction of guinea pig

Zahid Hussain, Kwang Won Rhee, Young Ju Lee, HyoJin Park

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background/Aims: Opioid induced bowel dysfunction (OIBD) is associated with decreased gastrointestinal (GI) propulsive activity due to intake of opioid analgesics. DA-9701, a novel prokinetic agent formulated with Pharbitis Semen and Corydalis Tuber has promising effects on GI motor function. Therefore, we aim to evaluate the prokinetic effects of DA-9701 in an OIBD model of guinea pig. Methods: The ileal and distal colon muscle contraction in presence of different doses of DA-9701, morphine, and combination (morphine + DA- 9701) was measured by tissue bath study. The prokinetic effect of DA-9701 was assessed by charcoal transit and fecal pellet output assay in an OIBD model of guinea pig. Results: DA-9701 significantly increased the amplitude and area under the curve of ileal muscle contraction, while there was insignificant effect on the distal colon compared to the control. The maximal amplitude of ileal muscle contraction was acquired at a concentration of 10 μg/mL of DA-9701. In contrast, morphine significantly decreased the amplitude of ileal and distal colon muscle contraction compared to the control. Morphine delayed both upper (P < 0.01) and lower (P < 0.05) GI transit, and delayed GI transit was restored by the administration of DA-9701. Morphine induced reduction of contractility was significantly ameliorated by addition of DA-9701 in both ileal and distal colon muscles. Conclusions: DA-9701 significantly increased the amplitude of contraction of the ileal muscle, however the distal colon muscle contraction was insignificant. Additionally, it restored delayed upper and lower GI transit in an OIBD model of guinea pig, and it might prove to be a useful candidate drug in a clinical trial for OIBD.

Original languageEnglish
Pages (from-to)529-538
Number of pages10
JournalJournal of Neurogastroenterology and Motility
Volume22
Issue number3
DOIs
Publication statusPublished - 2016 Jan 1

Fingerprint

Opioid Analgesics
Guinea Pigs
Muscle Contraction
Morphine
Gastrointestinal Transit
Colon
Corydalis
DA-9701
Charcoal
Semen
Baths
Area Under Curve
Clinical Trials
Muscles
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Clinical Neurology
  • Gastroenterology

Cite this

Hussain, Zahid ; Rhee, Kwang Won ; Lee, Young Ju ; Park, HyoJin. / The effect of DA-9701 in Opioid-induced bowel dysfunction of guinea pig. In: Journal of Neurogastroenterology and Motility. 2016 ; Vol. 22, No. 3. pp. 529-538.
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abstract = "Background/Aims: Opioid induced bowel dysfunction (OIBD) is associated with decreased gastrointestinal (GI) propulsive activity due to intake of opioid analgesics. DA-9701, a novel prokinetic agent formulated with Pharbitis Semen and Corydalis Tuber has promising effects on GI motor function. Therefore, we aim to evaluate the prokinetic effects of DA-9701 in an OIBD model of guinea pig. Methods: The ileal and distal colon muscle contraction in presence of different doses of DA-9701, morphine, and combination (morphine + DA- 9701) was measured by tissue bath study. The prokinetic effect of DA-9701 was assessed by charcoal transit and fecal pellet output assay in an OIBD model of guinea pig. Results: DA-9701 significantly increased the amplitude and area under the curve of ileal muscle contraction, while there was insignificant effect on the distal colon compared to the control. The maximal amplitude of ileal muscle contraction was acquired at a concentration of 10 μg/mL of DA-9701. In contrast, morphine significantly decreased the amplitude of ileal and distal colon muscle contraction compared to the control. Morphine delayed both upper (P < 0.01) and lower (P < 0.05) GI transit, and delayed GI transit was restored by the administration of DA-9701. Morphine induced reduction of contractility was significantly ameliorated by addition of DA-9701 in both ileal and distal colon muscles. Conclusions: DA-9701 significantly increased the amplitude of contraction of the ileal muscle, however the distal colon muscle contraction was insignificant. Additionally, it restored delayed upper and lower GI transit in an OIBD model of guinea pig, and it might prove to be a useful candidate drug in a clinical trial for OIBD.",
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The effect of DA-9701 in Opioid-induced bowel dysfunction of guinea pig. / Hussain, Zahid; Rhee, Kwang Won; Lee, Young Ju; Park, HyoJin.

In: Journal of Neurogastroenterology and Motility, Vol. 22, No. 3, 01.01.2016, p. 529-538.

Research output: Contribution to journalArticle

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