The effect of epigallocatechin-3-gallate (EGCG) on human alveolar bone cells both in vitro and in vivo

Yon Joo Mah, JeSeon Song, Seong Oh Kim, Jae Ho Lee, Mijeong Jeon, Ui-Won Jung, Seok Jun Moon, Jeong Hee Kim, Hyung Jun Choi

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Abstract

Objective The effects of epigallocatechin-3-gallate (EGCG), a major catechin in green tea, on human and mouse osteoblasts remain controversial. This study investigated the direct effects of EGCG on human alveolar bone-derived cells (hABCs) both in vitro and in vivo. Design hABCs which were collected from eight children (aged 7-9 years, seven males and one female) were treated with EGCG at various concentrations (1, 5, 10, 25, and 50 μM), and a proliferation assay, flow cytometric analysis for apoptosis evaluation, migration assay, and in vitro osteogenic differentiation were performed. hABCs that were pretreated with 10 μM EGCG and mixed with calcium phosphate carrier combined with EGCG (0.1, 0.5, or 1.5 mg) in vivo were transplanted into immunodeficient mouse. Histological staining, quantitative gene expressions, and alkaline phosphatase activity were evaluated in the retrieved transplants. Results The proliferation and migration were decreased when EGCG was present at over 25 μM. The osteogenic differentiation increased slightly when EGCG was present at up to 10 μM, and clearly decreased for higher concentrations of EGCG. In vivo, the potential for hard-tissue formation was slightly higher for the group with 0.1 mg of EGCG than for the control group, and decreased sharply for higher concentrations of EGCG. Conclusion The present observations suggest that EGCG at a low concentration can slightly enhance the osteogenic effect in vivo, whereas at a higher concentration it can prevent the osteogenic differentiation of hABCs both in vitro and in vivo.

Original languageEnglish
Pages (from-to)539-549
Number of pages11
JournalArchives of Oral Biology
Volume59
Issue number5
DOIs
Publication statusPublished - 2014 Jan 1

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Alveolar Epithelial Cells
Bone and Bones
epigallocatechin gallate
In Vitro Techniques
Catechin
Tea
Osteoblasts
Alkaline Phosphatase

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this

Mah, Yon Joo ; Song, JeSeon ; Kim, Seong Oh ; Lee, Jae Ho ; Jeon, Mijeong ; Jung, Ui-Won ; Moon, Seok Jun ; Kim, Jeong Hee ; Choi, Hyung Jun. / The effect of epigallocatechin-3-gallate (EGCG) on human alveolar bone cells both in vitro and in vivo. In: Archives of Oral Biology. 2014 ; Vol. 59, No. 5. pp. 539-549.
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abstract = "Objective The effects of epigallocatechin-3-gallate (EGCG), a major catechin in green tea, on human and mouse osteoblasts remain controversial. This study investigated the direct effects of EGCG on human alveolar bone-derived cells (hABCs) both in vitro and in vivo. Design hABCs which were collected from eight children (aged 7-9 years, seven males and one female) were treated with EGCG at various concentrations (1, 5, 10, 25, and 50 μM), and a proliferation assay, flow cytometric analysis for apoptosis evaluation, migration assay, and in vitro osteogenic differentiation were performed. hABCs that were pretreated with 10 μM EGCG and mixed with calcium phosphate carrier combined with EGCG (0.1, 0.5, or 1.5 mg) in vivo were transplanted into immunodeficient mouse. Histological staining, quantitative gene expressions, and alkaline phosphatase activity were evaluated in the retrieved transplants. Results The proliferation and migration were decreased when EGCG was present at over 25 μM. The osteogenic differentiation increased slightly when EGCG was present at up to 10 μM, and clearly decreased for higher concentrations of EGCG. In vivo, the potential for hard-tissue formation was slightly higher for the group with 0.1 mg of EGCG than for the control group, and decreased sharply for higher concentrations of EGCG. Conclusion The present observations suggest that EGCG at a low concentration can slightly enhance the osteogenic effect in vivo, whereas at a higher concentration it can prevent the osteogenic differentiation of hABCs both in vitro and in vivo.",
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The effect of epigallocatechin-3-gallate (EGCG) on human alveolar bone cells both in vitro and in vivo. / Mah, Yon Joo; Song, JeSeon; Kim, Seong Oh; Lee, Jae Ho; Jeon, Mijeong; Jung, Ui-Won; Moon, Seok Jun; Kim, Jeong Hee; Choi, Hyung Jun.

In: Archives of Oral Biology, Vol. 59, No. 5, 01.01.2014, p. 539-549.

Research output: Contribution to journalArticle

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AU - Mah, Yon Joo

AU - Song, JeSeon

AU - Kim, Seong Oh

AU - Lee, Jae Ho

AU - Jeon, Mijeong

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AU - Moon, Seok Jun

AU - Kim, Jeong Hee

AU - Choi, Hyung Jun

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N2 - Objective The effects of epigallocatechin-3-gallate (EGCG), a major catechin in green tea, on human and mouse osteoblasts remain controversial. This study investigated the direct effects of EGCG on human alveolar bone-derived cells (hABCs) both in vitro and in vivo. Design hABCs which were collected from eight children (aged 7-9 years, seven males and one female) were treated with EGCG at various concentrations (1, 5, 10, 25, and 50 μM), and a proliferation assay, flow cytometric analysis for apoptosis evaluation, migration assay, and in vitro osteogenic differentiation were performed. hABCs that were pretreated with 10 μM EGCG and mixed with calcium phosphate carrier combined with EGCG (0.1, 0.5, or 1.5 mg) in vivo were transplanted into immunodeficient mouse. Histological staining, quantitative gene expressions, and alkaline phosphatase activity were evaluated in the retrieved transplants. Results The proliferation and migration were decreased when EGCG was present at over 25 μM. The osteogenic differentiation increased slightly when EGCG was present at up to 10 μM, and clearly decreased for higher concentrations of EGCG. In vivo, the potential for hard-tissue formation was slightly higher for the group with 0.1 mg of EGCG than for the control group, and decreased sharply for higher concentrations of EGCG. Conclusion The present observations suggest that EGCG at a low concentration can slightly enhance the osteogenic effect in vivo, whereas at a higher concentration it can prevent the osteogenic differentiation of hABCs both in vitro and in vivo.

AB - Objective The effects of epigallocatechin-3-gallate (EGCG), a major catechin in green tea, on human and mouse osteoblasts remain controversial. This study investigated the direct effects of EGCG on human alveolar bone-derived cells (hABCs) both in vitro and in vivo. Design hABCs which were collected from eight children (aged 7-9 years, seven males and one female) were treated with EGCG at various concentrations (1, 5, 10, 25, and 50 μM), and a proliferation assay, flow cytometric analysis for apoptosis evaluation, migration assay, and in vitro osteogenic differentiation were performed. hABCs that were pretreated with 10 μM EGCG and mixed with calcium phosphate carrier combined with EGCG (0.1, 0.5, or 1.5 mg) in vivo were transplanted into immunodeficient mouse. Histological staining, quantitative gene expressions, and alkaline phosphatase activity were evaluated in the retrieved transplants. Results The proliferation and migration were decreased when EGCG was present at over 25 μM. The osteogenic differentiation increased slightly when EGCG was present at up to 10 μM, and clearly decreased for higher concentrations of EGCG. In vivo, the potential for hard-tissue formation was slightly higher for the group with 0.1 mg of EGCG than for the control group, and decreased sharply for higher concentrations of EGCG. Conclusion The present observations suggest that EGCG at a low concentration can slightly enhance the osteogenic effect in vivo, whereas at a higher concentration it can prevent the osteogenic differentiation of hABCs both in vitro and in vivo.

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