The effect of human serum albumin on the extended storage of human oral keratinocyte viability under mild hypothermia

Eun Ju Lee, Seung Ae Lee, Jin Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Isolated oral keratinocytes in suspension provide a number of advantages for use in maxillofacial surgery, however, the poor stability of this cell preparation at physiological temperatures is an apparent barrier preventing their use. The purpose of the present study was to evaluate whether human serum albumin (HSA) could serve as an effective constituent of a storage medium to enhance human oral keratinocyte (HOK) viability under conditions of mild hypothermia. Primary human oral keratinocytes were isolated from small pieces of the non-inflamed gingival tissues obtained during the extraction of the third molars of patients. HOK were cultured on collagen type I-coated culture dishes in keratinocyte growth medium (KGM). After the trypsinization of a culture dish (passage 2 or 3), freshly isolated HOK were stored for 24, 48, and 72 h at 4°C or at room temperature in KGM, saline, Dulbecco's modified Eagle's medium (DMEM), saline supplemented with 10% HSA or DMEM supplemented with 10% (v/v) HSA under one atmosphere pressure. After storage, HOK cell survival was determined by dye exclusion using trypan blue and colony-forming assay and cell cycle change was obtained by flow cytometry. Highest cell viability was obtained in saline supplemented with 10% HSA and DMEM supplemented with 10% (v/v) HSA at 4°C and at room temperature. Under these conditions no significant decline in keratinocyte viability was observed for at least 48 h. The cell cycle profiles of these cells were also maintained for at least 48 h at room temperature. These observations demonstrate that HSA might be better at preserving the viability of HOK stored under hypothermic and mild hypothermic conditions up to 48 h.

Original languageEnglish
Pages (from-to)103-111
Number of pages9
JournalCryobiology
Volume50
Issue number1
DOIs
Publication statusPublished - 2005 Jan 1

Fingerprint

Hypothermia
hypothermia
keratinocytes
Keratinocytes
Serum Albumin
mouth
viability
eagles
Eagles
Cells
ambient temperature
Temperature
cell viability
cell cycle
culture media
human serum albumin
Cell Survival
Cell Cycle
Trypan Blue
Flow cytometry

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

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title = "The effect of human serum albumin on the extended storage of human oral keratinocyte viability under mild hypothermia",
abstract = "Isolated oral keratinocytes in suspension provide a number of advantages for use in maxillofacial surgery, however, the poor stability of this cell preparation at physiological temperatures is an apparent barrier preventing their use. The purpose of the present study was to evaluate whether human serum albumin (HSA) could serve as an effective constituent of a storage medium to enhance human oral keratinocyte (HOK) viability under conditions of mild hypothermia. Primary human oral keratinocytes were isolated from small pieces of the non-inflamed gingival tissues obtained during the extraction of the third molars of patients. HOK were cultured on collagen type I-coated culture dishes in keratinocyte growth medium (KGM). After the trypsinization of a culture dish (passage 2 or 3), freshly isolated HOK were stored for 24, 48, and 72 h at 4°C or at room temperature in KGM, saline, Dulbecco's modified Eagle's medium (DMEM), saline supplemented with 10{\%} HSA or DMEM supplemented with 10{\%} (v/v) HSA under one atmosphere pressure. After storage, HOK cell survival was determined by dye exclusion using trypan blue and colony-forming assay and cell cycle change was obtained by flow cytometry. Highest cell viability was obtained in saline supplemented with 10{\%} HSA and DMEM supplemented with 10{\%} (v/v) HSA at 4°C and at room temperature. Under these conditions no significant decline in keratinocyte viability was observed for at least 48 h. The cell cycle profiles of these cells were also maintained for at least 48 h at room temperature. These observations demonstrate that HSA might be better at preserving the viability of HOK stored under hypothermic and mild hypothermic conditions up to 48 h.",
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The effect of human serum albumin on the extended storage of human oral keratinocyte viability under mild hypothermia. / Lee, Eun Ju; Lee, Seung Ae; Kim, Jin.

In: Cryobiology, Vol. 50, No. 1, 01.01.2005, p. 103-111.

Research output: Contribution to journalArticle

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