The effect of metformin on alveolar bone in ligature-induced periodontitis in rats: A pilot study

Eun Jung Bak, Hong Gyu Park, Minyoung Kim, Sung Whan Kim, Sungwuk Kim, Seong Ho Choi, Jeong Heon Cha, Yun Jung Yoo

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Background: The use of metformin, an antidiabetic agent, is associated with a reduced risk of fractures in patients with diabetes, suggesting that metformin exerts a beneficial effect on bone tissues. The objective of this study was to assess the effect of metformin on alveolar bone loss in ligature-induced periodontitis and osteoblast, osteoclast, and adipocyte differentiation. Methods: Periodontitis was induced by a ligature around the mandibular firstmolar of each rat. The rats were divided into twogroups: 1) rats with ligature receiving a vehicle (n = 5), and 2) rats with ligature receiving metformin (n = 5). On day 10, after the induction of periodontitis, the alveolar bone volume between the first and second molar was determined via microcomputed tomography. The effect of metformin on osteoblast, osteoclast, and adipocyte differentiation was assessed using MC3T3-E1, cocultures of mouse bone marrow cells and calvaria-derived osteoblasts, and 3T3-L1/C3H10T1/2 cells, respectively. Osteoblast, osteoclast, and adipocyte differentiationwas estimated by the degree of mineralization, the formation of tartrate-resistant acid phosphatase-positive multinucleated cells, and the accumulation of triglycerides, respectively. Results: In ligature-induced periodontitis, themetformin treatment of rats induced a significant reduction in alveolar bone loss compared to vehicle-treated rats.With regard to osteoblast differentiation, metformin augmented the mineralization of MC3T3-E1 cells approximately two-fold over the non-treated cells. However, metformin was shown to exert no effects on osteoclast formation induced by 1,25-dihydroxyvitamin D3, lipopolysaccharide, and prostaglandin E2. Moreover, metformin exerted no effect on adipocyte differentiation. Conclusion: Our findings suggest that metforminmay exert a beneficial effect on alveolar bone in periodontitis by increasing osteoblast differentiation. J Periodontol 2010;81:412-419.

Original languageEnglish
Pages (from-to)412-419
Number of pages8
JournalJournal of Periodontology
Volume81
Issue number3
DOIs
Publication statusPublished - 2010 Mar 1

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Metformin
Periodontitis
Ligation
Osteoblasts
Bone and Bones
Osteoclasts
Adipocytes
Alveolar Bone Loss
X-Ray Microtomography
Calcitriol
Coculture Techniques
Dinoprostone
Hypoglycemic Agents
Skull
Bone Marrow Cells
Lipopolysaccharides
Triglycerides

All Science Journal Classification (ASJC) codes

  • Periodontics

Cite this

Bak, Eun Jung ; Park, Hong Gyu ; Kim, Minyoung ; Kim, Sung Whan ; Kim, Sungwuk ; Choi, Seong Ho ; Cha, Jeong Heon ; Yoo, Yun Jung. / The effect of metformin on alveolar bone in ligature-induced periodontitis in rats : A pilot study. In: Journal of Periodontology. 2010 ; Vol. 81, No. 3. pp. 412-419.
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abstract = "Background: The use of metformin, an antidiabetic agent, is associated with a reduced risk of fractures in patients with diabetes, suggesting that metformin exerts a beneficial effect on bone tissues. The objective of this study was to assess the effect of metformin on alveolar bone loss in ligature-induced periodontitis and osteoblast, osteoclast, and adipocyte differentiation. Methods: Periodontitis was induced by a ligature around the mandibular firstmolar of each rat. The rats were divided into twogroups: 1) rats with ligature receiving a vehicle (n = 5), and 2) rats with ligature receiving metformin (n = 5). On day 10, after the induction of periodontitis, the alveolar bone volume between the first and second molar was determined via microcomputed tomography. The effect of metformin on osteoblast, osteoclast, and adipocyte differentiation was assessed using MC3T3-E1, cocultures of mouse bone marrow cells and calvaria-derived osteoblasts, and 3T3-L1/C3H10T1/2 cells, respectively. Osteoblast, osteoclast, and adipocyte differentiationwas estimated by the degree of mineralization, the formation of tartrate-resistant acid phosphatase-positive multinucleated cells, and the accumulation of triglycerides, respectively. Results: In ligature-induced periodontitis, themetformin treatment of rats induced a significant reduction in alveolar bone loss compared to vehicle-treated rats.With regard to osteoblast differentiation, metformin augmented the mineralization of MC3T3-E1 cells approximately two-fold over the non-treated cells. However, metformin was shown to exert no effects on osteoclast formation induced by 1,25-dihydroxyvitamin D3, lipopolysaccharide, and prostaglandin E2. Moreover, metformin exerted no effect on adipocyte differentiation. Conclusion: Our findings suggest that metforminmay exert a beneficial effect on alveolar bone in periodontitis by increasing osteoblast differentiation. J Periodontol 2010;81:412-419.",
author = "Bak, {Eun Jung} and Park, {Hong Gyu} and Minyoung Kim and Kim, {Sung Whan} and Sungwuk Kim and Choi, {Seong Ho} and Cha, {Jeong Heon} and Yoo, {Yun Jung}",
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The effect of metformin on alveolar bone in ligature-induced periodontitis in rats : A pilot study. / Bak, Eun Jung; Park, Hong Gyu; Kim, Minyoung; Kim, Sung Whan; Kim, Sungwuk; Choi, Seong Ho; Cha, Jeong Heon; Yoo, Yun Jung.

In: Journal of Periodontology, Vol. 81, No. 3, 01.03.2010, p. 412-419.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The effect of metformin on alveolar bone in ligature-induced periodontitis in rats

T2 - A pilot study

AU - Bak, Eun Jung

AU - Park, Hong Gyu

AU - Kim, Minyoung

AU - Kim, Sung Whan

AU - Kim, Sungwuk

AU - Choi, Seong Ho

AU - Cha, Jeong Heon

AU - Yoo, Yun Jung

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N2 - Background: The use of metformin, an antidiabetic agent, is associated with a reduced risk of fractures in patients with diabetes, suggesting that metformin exerts a beneficial effect on bone tissues. The objective of this study was to assess the effect of metformin on alveolar bone loss in ligature-induced periodontitis and osteoblast, osteoclast, and adipocyte differentiation. Methods: Periodontitis was induced by a ligature around the mandibular firstmolar of each rat. The rats were divided into twogroups: 1) rats with ligature receiving a vehicle (n = 5), and 2) rats with ligature receiving metformin (n = 5). On day 10, after the induction of periodontitis, the alveolar bone volume between the first and second molar was determined via microcomputed tomography. The effect of metformin on osteoblast, osteoclast, and adipocyte differentiation was assessed using MC3T3-E1, cocultures of mouse bone marrow cells and calvaria-derived osteoblasts, and 3T3-L1/C3H10T1/2 cells, respectively. Osteoblast, osteoclast, and adipocyte differentiationwas estimated by the degree of mineralization, the formation of tartrate-resistant acid phosphatase-positive multinucleated cells, and the accumulation of triglycerides, respectively. Results: In ligature-induced periodontitis, themetformin treatment of rats induced a significant reduction in alveolar bone loss compared to vehicle-treated rats.With regard to osteoblast differentiation, metformin augmented the mineralization of MC3T3-E1 cells approximately two-fold over the non-treated cells. However, metformin was shown to exert no effects on osteoclast formation induced by 1,25-dihydroxyvitamin D3, lipopolysaccharide, and prostaglandin E2. Moreover, metformin exerted no effect on adipocyte differentiation. Conclusion: Our findings suggest that metforminmay exert a beneficial effect on alveolar bone in periodontitis by increasing osteoblast differentiation. J Periodontol 2010;81:412-419.

AB - Background: The use of metformin, an antidiabetic agent, is associated with a reduced risk of fractures in patients with diabetes, suggesting that metformin exerts a beneficial effect on bone tissues. The objective of this study was to assess the effect of metformin on alveolar bone loss in ligature-induced periodontitis and osteoblast, osteoclast, and adipocyte differentiation. Methods: Periodontitis was induced by a ligature around the mandibular firstmolar of each rat. The rats were divided into twogroups: 1) rats with ligature receiving a vehicle (n = 5), and 2) rats with ligature receiving metformin (n = 5). On day 10, after the induction of periodontitis, the alveolar bone volume between the first and second molar was determined via microcomputed tomography. The effect of metformin on osteoblast, osteoclast, and adipocyte differentiation was assessed using MC3T3-E1, cocultures of mouse bone marrow cells and calvaria-derived osteoblasts, and 3T3-L1/C3H10T1/2 cells, respectively. Osteoblast, osteoclast, and adipocyte differentiationwas estimated by the degree of mineralization, the formation of tartrate-resistant acid phosphatase-positive multinucleated cells, and the accumulation of triglycerides, respectively. Results: In ligature-induced periodontitis, themetformin treatment of rats induced a significant reduction in alveolar bone loss compared to vehicle-treated rats.With regard to osteoblast differentiation, metformin augmented the mineralization of MC3T3-E1 cells approximately two-fold over the non-treated cells. However, metformin was shown to exert no effects on osteoclast formation induced by 1,25-dihydroxyvitamin D3, lipopolysaccharide, and prostaglandin E2. Moreover, metformin exerted no effect on adipocyte differentiation. Conclusion: Our findings suggest that metforminmay exert a beneficial effect on alveolar bone in periodontitis by increasing osteoblast differentiation. J Periodontol 2010;81:412-419.

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