The effect of peripheral CRF peptide and water avoidance stress on colonic and gastric transit in Guinea pigs

Zahid Hussain, Hae Won Kim, Cheal Wung Huh, Young Ju Lee, HyoJin Park

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Functional dyspepsia (FD) and irritable bowel syndrome (IBS) are common gastrointestinal (GI) diseases; however, there is frequent overlap between FD and IBS patients. Emerging evidence links the activation of corticotropin releasing factor (CRF) receptors with stress-related alterations of gastric and colonic motor function. Therefore, we investigated the effect of peripheral CRF peptide and water avoidance stress (WAS) on upper and lower GI transit in guinea pigs. Dosages 1, 3, and 10 μg/kg of CRF were injected intraper-itoneally (IP) in fasted guinea pigs 30 minutes prior to the intragastric administration of charcoal mix to measure upper GI transit. Colonic transits in non-fasted guinea pigs were assessed by fecal pellet output assay after above IP CRF doses. Blockade of CRF receptors by Astressin, and its effect on GI transit was also analyzed. Guinea pigs were subjected to WAS to measure gastrocolonic transit in different sets of experiments. Dose 10 μg/kg of CRF significantly inhibited upper GI transit. In contrast, there was dose dependent acceleration of the colonic transit. Remarkably, pretreatment of astressin significantly reverses the effect of CRF peptide on GI transit. WAS significantly increase colonic transit, but failed to accelerate upper GI transit. Peripheral CRF peptide significantly suppressed upper GI transit and accelerated colon transit, while central CRF involved WAS stimulated only colonic transit. Therefore, peripheral CRF could be utilized to establish the animal model of overlap syndrome.

Original languageEnglish
Pages (from-to)872-877
Number of pages6
JournalYonsei medical journal
Volume58
Issue number4
DOIs
Publication statusPublished - 2017 Jul 1

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Gastrointestinal Transit
Corticotropin-Releasing Hormone
Dehydration
Stomach
Guinea Pigs
Peptides
Corticotropin-Releasing Hormone Receptors
Irritable Bowel Syndrome
Dyspepsia
Gastrointestinal Diseases
Charcoal
Colon
Animal Models

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Hussain, Zahid ; Kim, Hae Won ; Huh, Cheal Wung ; Lee, Young Ju ; Park, HyoJin. / The effect of peripheral CRF peptide and water avoidance stress on colonic and gastric transit in Guinea pigs. In: Yonsei medical journal. 2017 ; Vol. 58, No. 4. pp. 872-877.
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The effect of peripheral CRF peptide and water avoidance stress on colonic and gastric transit in Guinea pigs. / Hussain, Zahid; Kim, Hae Won; Huh, Cheal Wung; Lee, Young Ju; Park, HyoJin.

In: Yonsei medical journal, Vol. 58, No. 4, 01.07.2017, p. 872-877.

Research output: Contribution to journalArticle

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