The effect of varying the particle size of beta tricalcium phosphate carrier of recombinant human bone morphogenetic protein-4 on bone formation in rat calvarial defects

Ui Won Jung, Seong Yong Choi, Eun Kyoung Pang, Chang Sung Kim, Seong Ho Choi, Kyoo Sung Cho

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27 Citations (Scopus)

Abstract

Background: Beta tricalcium phosphate (β-TCP) has been developed as one of the carriers of recombinant human bone morphogenetic protein (rhBMP). However, it is not known whether the particle size of β-TCP is related to its resorption rate and the degree of bone formation. The purpose of this study was to evaluate the effect of using β-TCP with different particle sizes on the ability of rhBMP-4 to enhance bone formation in the rat calvarial defect model. Methods: Calvarial, 8-mm-diameter, critical-size defects were created in 100 male Sprague-Dawley rats. Five groups of 20 animals each received either rhBMP-4 (2.5 μg) using β-TCP with a particle size of 50 to 150 μm, rhBMP-4 (2.5 μg) using β3-TCP with a particle size of 150 to 500 μm, a β-TCP control with a particle size of 50 to 150 μm, a β-TCP control with a particle size of 150 to 500 μm, or a sham-surgery control, respectively, and were evaluated by measuring their histologic and histometric parameters following a 2- and 8-week healing interval. Results: There were no significant differences in the defect closure, new bone area, or augmented area between either the two rhBMP-4/β-TCP groups or between the two β-TCP control groups at 2 and 8 weeks. Conclusions: rhBMP-4 combined with either small- or large-particle β-TCP had a significant effect on the induction of bone formation compared to either a small- or large-particle β-TCP control or a sham-surgery control. Within the parameters of this study, varying the particle size of β-TCP did not seem to have a significant effect on bone formation.

Original languageEnglish
Pages (from-to)765-772
Number of pages8
JournalJournal of Periodontology
Volume77
Issue number5
DOIs
Publication statusPublished - 2006 May 1

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Phosphate Transport Proteins
Particle Size
Osteogenesis
Bone Morphogenetic Proteins
human BMP4 protein
beta-tricalcium phosphate
Sprague Dawley Rats
Bone and Bones
Control Groups

All Science Journal Classification (ASJC) codes

  • Periodontics

Cite this

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title = "The effect of varying the particle size of beta tricalcium phosphate carrier of recombinant human bone morphogenetic protein-4 on bone formation in rat calvarial defects",
abstract = "Background: Beta tricalcium phosphate (β-TCP) has been developed as one of the carriers of recombinant human bone morphogenetic protein (rhBMP). However, it is not known whether the particle size of β-TCP is related to its resorption rate and the degree of bone formation. The purpose of this study was to evaluate the effect of using β-TCP with different particle sizes on the ability of rhBMP-4 to enhance bone formation in the rat calvarial defect model. Methods: Calvarial, 8-mm-diameter, critical-size defects were created in 100 male Sprague-Dawley rats. Five groups of 20 animals each received either rhBMP-4 (2.5 μg) using β-TCP with a particle size of 50 to 150 μm, rhBMP-4 (2.5 μg) using β3-TCP with a particle size of 150 to 500 μm, a β-TCP control with a particle size of 50 to 150 μm, a β-TCP control with a particle size of 150 to 500 μm, or a sham-surgery control, respectively, and were evaluated by measuring their histologic and histometric parameters following a 2- and 8-week healing interval. Results: There were no significant differences in the defect closure, new bone area, or augmented area between either the two rhBMP-4/β-TCP groups or between the two β-TCP control groups at 2 and 8 weeks. Conclusions: rhBMP-4 combined with either small- or large-particle β-TCP had a significant effect on the induction of bone formation compared to either a small- or large-particle β-TCP control or a sham-surgery control. Within the parameters of this study, varying the particle size of β-TCP did not seem to have a significant effect on bone formation.",
author = "Jung, {Ui Won} and Choi, {Seong Yong} and Pang, {Eun Kyoung} and Kim, {Chang Sung} and Choi, {Seong Ho} and Cho, {Kyoo Sung}",
year = "2006",
month = "5",
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doi = "10.1902/jop.2006.050268",
language = "English",
volume = "77",
pages = "765--772",
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T1 - The effect of varying the particle size of beta tricalcium phosphate carrier of recombinant human bone morphogenetic protein-4 on bone formation in rat calvarial defects

AU - Jung, Ui Won

AU - Choi, Seong Yong

AU - Pang, Eun Kyoung

AU - Kim, Chang Sung

AU - Choi, Seong Ho

AU - Cho, Kyoo Sung

PY - 2006/5/1

Y1 - 2006/5/1

N2 - Background: Beta tricalcium phosphate (β-TCP) has been developed as one of the carriers of recombinant human bone morphogenetic protein (rhBMP). However, it is not known whether the particle size of β-TCP is related to its resorption rate and the degree of bone formation. The purpose of this study was to evaluate the effect of using β-TCP with different particle sizes on the ability of rhBMP-4 to enhance bone formation in the rat calvarial defect model. Methods: Calvarial, 8-mm-diameter, critical-size defects were created in 100 male Sprague-Dawley rats. Five groups of 20 animals each received either rhBMP-4 (2.5 μg) using β-TCP with a particle size of 50 to 150 μm, rhBMP-4 (2.5 μg) using β3-TCP with a particle size of 150 to 500 μm, a β-TCP control with a particle size of 50 to 150 μm, a β-TCP control with a particle size of 150 to 500 μm, or a sham-surgery control, respectively, and were evaluated by measuring their histologic and histometric parameters following a 2- and 8-week healing interval. Results: There were no significant differences in the defect closure, new bone area, or augmented area between either the two rhBMP-4/β-TCP groups or between the two β-TCP control groups at 2 and 8 weeks. Conclusions: rhBMP-4 combined with either small- or large-particle β-TCP had a significant effect on the induction of bone formation compared to either a small- or large-particle β-TCP control or a sham-surgery control. Within the parameters of this study, varying the particle size of β-TCP did not seem to have a significant effect on bone formation.

AB - Background: Beta tricalcium phosphate (β-TCP) has been developed as one of the carriers of recombinant human bone morphogenetic protein (rhBMP). However, it is not known whether the particle size of β-TCP is related to its resorption rate and the degree of bone formation. The purpose of this study was to evaluate the effect of using β-TCP with different particle sizes on the ability of rhBMP-4 to enhance bone formation in the rat calvarial defect model. Methods: Calvarial, 8-mm-diameter, critical-size defects were created in 100 male Sprague-Dawley rats. Five groups of 20 animals each received either rhBMP-4 (2.5 μg) using β-TCP with a particle size of 50 to 150 μm, rhBMP-4 (2.5 μg) using β3-TCP with a particle size of 150 to 500 μm, a β-TCP control with a particle size of 50 to 150 μm, a β-TCP control with a particle size of 150 to 500 μm, or a sham-surgery control, respectively, and were evaluated by measuring their histologic and histometric parameters following a 2- and 8-week healing interval. Results: There were no significant differences in the defect closure, new bone area, or augmented area between either the two rhBMP-4/β-TCP groups or between the two β-TCP control groups at 2 and 8 weeks. Conclusions: rhBMP-4 combined with either small- or large-particle β-TCP had a significant effect on the induction of bone formation compared to either a small- or large-particle β-TCP control or a sham-surgery control. Within the parameters of this study, varying the particle size of β-TCP did not seem to have a significant effect on bone formation.

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JF - Journal of Periodontology

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