The objective of this study was to find out if suppression of NF-kB complex function by p65-TMD-linked PTD could reduce host inflammation and bone resorption at peri-implantitis sites in rats. Twenty-one male 5-week-old SD rats were divided into three groups: untreated control group (A), silk-induced peri-implantitis group (B), and nt (nucleus transducible)-p65-TMD-treated, silk-induced peri-implantitis group (C). Implant sulcus of a rat in group C were divided into two groups, namely group Cp and Cb. Palatal implant sulcus where nt-p65-TMD solution was applied with an insulin syringe were assigned to group Cp. Buccal implant sulcus without topical nt-p65-TMD application were assigned to group Cb. H&E staining, TRAP staining, and immunohistological staining were done. The crestal bone levels of group A were significantly higher than those of group B at p<0.01. The crestal bone levels of group Cp were significantly higher than those of group Cb at p<0.05. H-E staining showed increased apical migration of junctional epithelium and inflammatory cells in group Cb. TRAP staining revealed more multinucleated osteoclasts in group Cb. As for immunohistological staining, group Cb showed many IL-6-positive cells while group Cp had none. In this study, p65-TMD-linked PTD inhibited NF-kB functions and reduced inflammation and bone resorption at peri-implantitis sites in rats.
|Number of pages||11|
|Publication status||Published - 2021 Dec|
Bibliographical noteFunding Information:
The authors thank Osstem for providing bone screw implants (BSCH1404). Appreciation is extended to Ms. Ji-Hyun Woo and Kyung-Hwa Lee at the Catholic University of Korea, Uijeongbu St. Mary’s Hospital, for their aid in the animal experiment and data acquisition. We also want to thank Ms. YeunJu Kim at the Department of Prosthodontics, College of Dentistry, Yonsei University, for her aid in polishing the manuscript.
© 2021, The Author(s).
All Science Journal Classification (ASJC) codes
- Immunology and Allergy