The effects of recombinant human BMP-7, prepared from a COS-7 expression system, on the proliferation and differentiation of rat newborn calvarial osteoblasts

Dong Hee Lee, Hwal Suh, Dong Wook Han, Bong Joo Park, Jin Woo Lee, Jong Chul Park

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A family of proteins, the bone morphogenetic proteins (BMPs), which promote osteoblast differentiation and bone mineralization, have recently been identified. One, BMP-7, has shown the ability to induce cartilage and bone formation processes. In this report, the possibility that other cell lines, to CHO cells, may also be available as host cells for the expression of hBMP-7 was validated. Recombinant human BMP (rhBMP)-7 was produced in COS-7 cells, as a processed mature disulfide-linked homodimer, with an apparent molecular weight of 36,000. Examination of the expressions of the markers characteristic of osteoblast phenotypes showed that the rhBMP-7 specifically stimulated the inductions of alkaline phosphatase (ALP) (5-fold increase at 100 ng of rhBMP-7/ml), parathyroid hormone (PTH)-mediated intracellular cAMP production (4-fold increase at 100 ng of rhBMP-7/ml) and osteocalcin synthesis (5-fold increase at 100 ng of rhBMP-7/ml). In summary, the in vitro mineralization assay results provide evidence that the rhBMP-7 peptide, produced by COS-7 expression system, possesses intact biological activity. A similar pattern of biological activity was observed for the BMP-7 in COS-7 cells compared to the corresponding CHO cell expression system. Thus, these findings can be experimentally utilized for the production of rhBMPs for in vitro or in vivo studies.

Original languageEnglish
Pages (from-to)593-601
Number of pages9
JournalYonsei medical journal
Volume44
Issue number4
DOIs
Publication statusPublished - 2003 Aug 30

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Bone Morphogenetic Protein 7
Osteoblasts
CHO Cells
COS Cells
Physiologic Calcification
Bone Morphogenetic Proteins
Osteocalcin
human BMP7 protein
Parathyroid Hormone
Osteogenesis
Disulfides
Cartilage
Alkaline Phosphatase
Molecular Weight
Phenotype
Cell Line
Peptides

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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title = "The effects of recombinant human BMP-7, prepared from a COS-7 expression system, on the proliferation and differentiation of rat newborn calvarial osteoblasts",
abstract = "A family of proteins, the bone morphogenetic proteins (BMPs), which promote osteoblast differentiation and bone mineralization, have recently been identified. One, BMP-7, has shown the ability to induce cartilage and bone formation processes. In this report, the possibility that other cell lines, to CHO cells, may also be available as host cells for the expression of hBMP-7 was validated. Recombinant human BMP (rhBMP)-7 was produced in COS-7 cells, as a processed mature disulfide-linked homodimer, with an apparent molecular weight of 36,000. Examination of the expressions of the markers characteristic of osteoblast phenotypes showed that the rhBMP-7 specifically stimulated the inductions of alkaline phosphatase (ALP) (5-fold increase at 100 ng of rhBMP-7/ml), parathyroid hormone (PTH)-mediated intracellular cAMP production (4-fold increase at 100 ng of rhBMP-7/ml) and osteocalcin synthesis (5-fold increase at 100 ng of rhBMP-7/ml). In summary, the in vitro mineralization assay results provide evidence that the rhBMP-7 peptide, produced by COS-7 expression system, possesses intact biological activity. A similar pattern of biological activity was observed for the BMP-7 in COS-7 cells compared to the corresponding CHO cell expression system. Thus, these findings can be experimentally utilized for the production of rhBMPs for in vitro or in vivo studies.",
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The effects of recombinant human BMP-7, prepared from a COS-7 expression system, on the proliferation and differentiation of rat newborn calvarial osteoblasts. / Lee, Dong Hee; Suh, Hwal; Han, Dong Wook; Park, Bong Joo; Lee, Jin Woo; Park, Jong Chul.

In: Yonsei medical journal, Vol. 44, No. 4, 30.08.2003, p. 593-601.

Research output: Contribution to journalArticle

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AU - Lee, Dong Hee

AU - Suh, Hwal

AU - Han, Dong Wook

AU - Park, Bong Joo

AU - Lee, Jin Woo

AU - Park, Jong Chul

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