The effects of storage conditions on the stability of house dust mite extracts

Kyoung Yong Jeong, Soo Young Choi, In Soo Han, Jae Hyun Lee, Joo Shil Lee, Chein Soo Hong, Jungwon Park

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Purpose: Allergen extracts from the house dust mite (HDM, Dermatophagoides pteronyssinus) are widely utilized for diagnosis and treatment of allergic diseases. It is known that allergen extracts degrade and lose potency when stored over time. Methods: This study aimed to determine the optimal conditions for stability of allergen extracts. This study was undertaken to investigate the optimal storage conditions for HDM extracts, the effects of adding 0.03% human serum albumin (HSA) and 50% glycerol were evaluated at -20°C, 4°C, and room temperature (RT). Changes in protein and group 1 major allergen (Der p 1) concentration, as well as allergenicity were measured over a 1 year period using the Bradford assay, twosite ELISA, and ELISA inhibition. Results: Protein concentrations decreased by 86%, 51%, and 6% at RT, 4°C, and -20°C, respectively, when stored in distilled water. Overall allergenicity remained high (89.9%) when the extracts was reconstituted in 50% glycerol solution, and was 93.1% when reconstituted in 50% glycerol and 0.03% HSA at RT. Allergenicity was decreased to 36.6% and 33.3%, however, reconstitution in DW or 0.03% HSA solution at RT, respectively. Allergenicity was remained high as 92.0%-97.0% when stored at 4°C regardless of the buffer conditions. Conclusions: Storage temperature is the most important factor in preserving allergenicity of HDM extracts, which is ideal at 4°C. The addition of 50% glycerol to the storage buffer was also found to play an important role in increasing the shelf-life of HDM extracts at RT.

Original languageEnglish
Pages (from-to)397-401
Number of pages5
JournalAllergy, Asthma and Immunology Research
Volume5
Issue number6
DOIs
Publication statusPublished - 2013 Nov 1

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Pyroglyphidae
Glycerol
Temperature
Serum Albumin
Allergens
Buffers
Enzyme-Linked Immunosorbent Assay
Dermatophagoides pteronyssinus
Dermatophagoides Antigens
Proteins
Water

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Pulmonary and Respiratory Medicine

Cite this

Jeong, Kyoung Yong ; Choi, Soo Young ; Han, In Soo ; Lee, Jae Hyun ; Lee, Joo Shil ; Hong, Chein Soo ; Park, Jungwon. / The effects of storage conditions on the stability of house dust mite extracts. In: Allergy, Asthma and Immunology Research. 2013 ; Vol. 5, No. 6. pp. 397-401.
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abstract = "Purpose: Allergen extracts from the house dust mite (HDM, Dermatophagoides pteronyssinus) are widely utilized for diagnosis and treatment of allergic diseases. It is known that allergen extracts degrade and lose potency when stored over time. Methods: This study aimed to determine the optimal conditions for stability of allergen extracts. This study was undertaken to investigate the optimal storage conditions for HDM extracts, the effects of adding 0.03{\%} human serum albumin (HSA) and 50{\%} glycerol were evaluated at -20°C, 4°C, and room temperature (RT). Changes in protein and group 1 major allergen (Der p 1) concentration, as well as allergenicity were measured over a 1 year period using the Bradford assay, twosite ELISA, and ELISA inhibition. Results: Protein concentrations decreased by 86{\%}, 51{\%}, and 6{\%} at RT, 4°C, and -20°C, respectively, when stored in distilled water. Overall allergenicity remained high (89.9{\%}) when the extracts was reconstituted in 50{\%} glycerol solution, and was 93.1{\%} when reconstituted in 50{\%} glycerol and 0.03{\%} HSA at RT. Allergenicity was decreased to 36.6{\%} and 33.3{\%}, however, reconstitution in DW or 0.03{\%} HSA solution at RT, respectively. Allergenicity was remained high as 92.0{\%}-97.0{\%} when stored at 4°C regardless of the buffer conditions. Conclusions: Storage temperature is the most important factor in preserving allergenicity of HDM extracts, which is ideal at 4°C. The addition of 50{\%} glycerol to the storage buffer was also found to play an important role in increasing the shelf-life of HDM extracts at RT.",
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The effects of storage conditions on the stability of house dust mite extracts. / Jeong, Kyoung Yong; Choi, Soo Young; Han, In Soo; Lee, Jae Hyun; Lee, Joo Shil; Hong, Chein Soo; Park, Jungwon.

In: Allergy, Asthma and Immunology Research, Vol. 5, No. 6, 01.11.2013, p. 397-401.

Research output: Contribution to journalArticle

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AU - Jeong, Kyoung Yong

AU - Choi, Soo Young

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AU - Lee, Jae Hyun

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AU - Hong, Chein Soo

AU - Park, Jungwon

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