The efficiency of RNA interference in Bursaphelenchus xylophilus

Jung Eun Park, Kyong Yun Lee, Se Jin Lee, Wan Suk Oh, Pan Young Jeong, Taeha Woo, Chang Bae Kim, Young-Ki Paik, Hyeon-Sook Koo

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

RNA Interference (RNAI) was performed on several essential genes in the pinewood nematode Bursaphelenchus xylophilus, which causes pine wilt disease. Double-stranded RNA (deRNA) was delivered to larvae or adult worms by soaking, electroporation, or microinjection. Soaking and electroporation of L2-L3 stage worms in solutions containing dsRNA for essential genes Induced over 250% lethality after 5 days, and gene-specific phenotypes were observed. This lethality agreed with significant reductions of the targeted transcripts, as assayed by reverse-transcription coupled with real time PCR. Microinjection was the most efficient routs as measured by the hatching rate of F1 embryos, which was reduced by 46%. When adult worms were soaked In dsRNA, lethality was induced in the F1 larvae, revealing the persistence of knockdown phenotypes. The penstrance of the RNA/phenotypes for essential genes was relatively low but consistent indicating that RNA/ should be useful for studying the in vivo functions of B. xylophilus gene products.

Original languageEnglish
Pages (from-to)81-86
Number of pages6
JournalMolecules and Cells
Volume26
Issue number1
Publication statusPublished - 2008 Jul 31

Fingerprint

Essential Genes
RNA Interference
Electroporation
Microinjections
Phenotype
Larva
RNA
Double-Stranded RNA
Genes
Reverse Transcription
Real-Time Polymerase Chain Reaction
Embryonic Structures

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Park, J. E., Lee, K. Y., Lee, S. J., Oh, W. S., Jeong, P. Y., Woo, T., ... Koo, H-S. (2008). The efficiency of RNA interference in Bursaphelenchus xylophilus. Molecules and Cells, 26(1), 81-86.
Park, Jung Eun ; Lee, Kyong Yun ; Lee, Se Jin ; Oh, Wan Suk ; Jeong, Pan Young ; Woo, Taeha ; Kim, Chang Bae ; Paik, Young-Ki ; Koo, Hyeon-Sook. / The efficiency of RNA interference in Bursaphelenchus xylophilus. In: Molecules and Cells. 2008 ; Vol. 26, No. 1. pp. 81-86.
@article{e45f4d99655e490eb200da34bf3da022,
title = "The efficiency of RNA interference in Bursaphelenchus xylophilus",
abstract = "RNA Interference (RNAI) was performed on several essential genes in the pinewood nematode Bursaphelenchus xylophilus, which causes pine wilt disease. Double-stranded RNA (deRNA) was delivered to larvae or adult worms by soaking, electroporation, or microinjection. Soaking and electroporation of L2-L3 stage worms in solutions containing dsRNA for essential genes Induced over 250{\%} lethality after 5 days, and gene-specific phenotypes were observed. This lethality agreed with significant reductions of the targeted transcripts, as assayed by reverse-transcription coupled with real time PCR. Microinjection was the most efficient routs as measured by the hatching rate of F1 embryos, which was reduced by 46{\%}. When adult worms were soaked In dsRNA, lethality was induced in the F1 larvae, revealing the persistence of knockdown phenotypes. The penstrance of the RNA/phenotypes for essential genes was relatively low but consistent indicating that RNA/ should be useful for studying the in vivo functions of B. xylophilus gene products.",
author = "Park, {Jung Eun} and Lee, {Kyong Yun} and Lee, {Se Jin} and Oh, {Wan Suk} and Jeong, {Pan Young} and Taeha Woo and Kim, {Chang Bae} and Young-Ki Paik and Hyeon-Sook Koo",
year = "2008",
month = "7",
day = "31",
language = "English",
volume = "26",
pages = "81--86",
journal = "Molecules and Cells",
issn = "1016-8478",
publisher = "Korean Society for Molecular and Cellular Biology",
number = "1",

}

Park, JE, Lee, KY, Lee, SJ, Oh, WS, Jeong, PY, Woo, T, Kim, CB, Paik, Y-K & Koo, H-S 2008, 'The efficiency of RNA interference in Bursaphelenchus xylophilus', Molecules and Cells, vol. 26, no. 1, pp. 81-86.

The efficiency of RNA interference in Bursaphelenchus xylophilus. / Park, Jung Eun; Lee, Kyong Yun; Lee, Se Jin; Oh, Wan Suk; Jeong, Pan Young; Woo, Taeha; Kim, Chang Bae; Paik, Young-Ki; Koo, Hyeon-Sook.

In: Molecules and Cells, Vol. 26, No. 1, 31.07.2008, p. 81-86.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The efficiency of RNA interference in Bursaphelenchus xylophilus

AU - Park, Jung Eun

AU - Lee, Kyong Yun

AU - Lee, Se Jin

AU - Oh, Wan Suk

AU - Jeong, Pan Young

AU - Woo, Taeha

AU - Kim, Chang Bae

AU - Paik, Young-Ki

AU - Koo, Hyeon-Sook

PY - 2008/7/31

Y1 - 2008/7/31

N2 - RNA Interference (RNAI) was performed on several essential genes in the pinewood nematode Bursaphelenchus xylophilus, which causes pine wilt disease. Double-stranded RNA (deRNA) was delivered to larvae or adult worms by soaking, electroporation, or microinjection. Soaking and electroporation of L2-L3 stage worms in solutions containing dsRNA for essential genes Induced over 250% lethality after 5 days, and gene-specific phenotypes were observed. This lethality agreed with significant reductions of the targeted transcripts, as assayed by reverse-transcription coupled with real time PCR. Microinjection was the most efficient routs as measured by the hatching rate of F1 embryos, which was reduced by 46%. When adult worms were soaked In dsRNA, lethality was induced in the F1 larvae, revealing the persistence of knockdown phenotypes. The penstrance of the RNA/phenotypes for essential genes was relatively low but consistent indicating that RNA/ should be useful for studying the in vivo functions of B. xylophilus gene products.

AB - RNA Interference (RNAI) was performed on several essential genes in the pinewood nematode Bursaphelenchus xylophilus, which causes pine wilt disease. Double-stranded RNA (deRNA) was delivered to larvae or adult worms by soaking, electroporation, or microinjection. Soaking and electroporation of L2-L3 stage worms in solutions containing dsRNA for essential genes Induced over 250% lethality after 5 days, and gene-specific phenotypes were observed. This lethality agreed with significant reductions of the targeted transcripts, as assayed by reverse-transcription coupled with real time PCR. Microinjection was the most efficient routs as measured by the hatching rate of F1 embryos, which was reduced by 46%. When adult worms were soaked In dsRNA, lethality was induced in the F1 larvae, revealing the persistence of knockdown phenotypes. The penstrance of the RNA/phenotypes for essential genes was relatively low but consistent indicating that RNA/ should be useful for studying the in vivo functions of B. xylophilus gene products.

UR - http://www.scopus.com/inward/record.url?scp=53449095232&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=53449095232&partnerID=8YFLogxK

M3 - Article

VL - 26

SP - 81

EP - 86

JO - Molecules and Cells

JF - Molecules and Cells

SN - 1016-8478

IS - 1

ER -

Park JE, Lee KY, Lee SJ, Oh WS, Jeong PY, Woo T et al. The efficiency of RNA interference in Bursaphelenchus xylophilus. Molecules and Cells. 2008 Jul 31;26(1):81-86.