The efficiency of RNA interference in Bursaphelenchus xylophilus

Jung Eun Park, Kyong Yun Lee, Se Jin Lee, Wan Suk Oh, Pan Young Jeong, Taeha Woo, Chang Bae Kim, Young Ki Paik, Hyeon Sook Koo

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31 Citations (Scopus)

Abstract

RNA Interference (RNAI) was performed on several essential genes in the pinewood nematode Bursaphelenchus xylophilus, which causes pine wilt disease. Double-stranded RNA (deRNA) was delivered to larvae or adult worms by soaking, electroporation, or microinjection. Soaking and electroporation of L2-L3 stage worms in solutions containing dsRNA for essential genes Induced over 250% lethality after 5 days, and gene-specific phenotypes were observed. This lethality agreed with significant reductions of the targeted transcripts, as assayed by reverse-transcription coupled with real time PCR. Microinjection was the most efficient routs as measured by the hatching rate of F1 embryos, which was reduced by 46%. When adult worms were soaked In dsRNA, lethality was induced in the F1 larvae, revealing the persistence of knockdown phenotypes. The penstrance of the RNA/phenotypes for essential genes was relatively low but consistent indicating that RNA/ should be useful for studying the in vivo functions of B. xylophilus gene products.

Original languageEnglish
Pages (from-to)81-86
Number of pages6
JournalMolecules and cells
Volume26
Issue number1
Publication statusPublished - 2008 Jul 31

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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    Park, J. E., Lee, K. Y., Lee, S. J., Oh, W. S., Jeong, P. Y., Woo, T., Kim, C. B., Paik, Y. K., & Koo, H. S. (2008). The efficiency of RNA interference in Bursaphelenchus xylophilus. Molecules and cells, 26(1), 81-86.