The expression of BAFF in the muscles of patients with dermatomyositis

Ahmi Baek, Hyung Jun Park, Sang Jun Na, Dong Suk Shim, Joon Shik Moon, Young Yang, Youngchul Choi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A B-cell activating factor of the tumor necrosis factor (TNF) family (BAFF) plays a crucial role in B-cell survival and maturation. An elevated serum BAFF level has been linked to several autoimmune diseases such as Sjögren syndrome, systemic lupus erythematosus and rheumatoid arthritis. Dermatomyositis (DM), one of autoimmune inflammatory myopathies, is characterized by inflammatory cell infiltration (CD4 + T cells and B cells) in skeletal muscle. Serum BAFF level was significantly high in DM, but the role of BAFF is not well understood. We investigated the role of BAFF in the immunopathogenesis of DM. To examine the transcriptional increase of BAFF gene expression, we performed RT-PCR analysis with skeletal muscle tissue that contained 4 controls and 9 patients with DM. Next, in order to detect BAFF expression and cellular localization in DM, we executed immunostaining in cryosection of biopsied muscle tissue with 4 controls and 8 patients and we adopted to double immunostaining to find which inflammatory cells expressed BAFF-receptor (BAFF-R). BAFF mRNA level was increased in DM patients compared with normal controls. BAFF expression was markedly increased at muscle fibers in the perifascicular area but not blood vessels. BAFF-R was expressed in inflammatory cells in skeletal muscle tissues of DM patients. We found that BAFF expression in muscle tissue may be associated with an increased number of CD4 + T cells and CD19 + B cells in DM. Our study results suggest that BAFF might play an important role in the pathogenesis of DM.

Original languageEnglish
Pages (from-to)96-100
Number of pages5
JournalJournal of Neuroimmunology
Volume249
Issue number1-2
DOIs
Publication statusPublished - 2012 Aug 15

Fingerprint

Dermatomyositis
Muscles
B-Cell Activation Factor Receptor
Skeletal Muscle
B-Lymphocytes
B-Cell Activating Factor
T-Lymphocytes
Myositis
Serum
Systemic Lupus Erythematosus
Autoimmune Diseases
Blood Vessels
Rheumatoid Arthritis
Cell Survival
Tumor Necrosis Factor-alpha
Gene Expression
Polymerase Chain Reaction
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

Cite this

Baek, Ahmi ; Park, Hyung Jun ; Na, Sang Jun ; Shim, Dong Suk ; Moon, Joon Shik ; Yang, Young ; Choi, Youngchul. / The expression of BAFF in the muscles of patients with dermatomyositis. In: Journal of Neuroimmunology. 2012 ; Vol. 249, No. 1-2. pp. 96-100.
@article{8d99f7eb0fce4f6bac151580c13ef0b2,
title = "The expression of BAFF in the muscles of patients with dermatomyositis",
abstract = "A B-cell activating factor of the tumor necrosis factor (TNF) family (BAFF) plays a crucial role in B-cell survival and maturation. An elevated serum BAFF level has been linked to several autoimmune diseases such as Sj{\"o}gren syndrome, systemic lupus erythematosus and rheumatoid arthritis. Dermatomyositis (DM), one of autoimmune inflammatory myopathies, is characterized by inflammatory cell infiltration (CD4 + T cells and B cells) in skeletal muscle. Serum BAFF level was significantly high in DM, but the role of BAFF is not well understood. We investigated the role of BAFF in the immunopathogenesis of DM. To examine the transcriptional increase of BAFF gene expression, we performed RT-PCR analysis with skeletal muscle tissue that contained 4 controls and 9 patients with DM. Next, in order to detect BAFF expression and cellular localization in DM, we executed immunostaining in cryosection of biopsied muscle tissue with 4 controls and 8 patients and we adopted to double immunostaining to find which inflammatory cells expressed BAFF-receptor (BAFF-R). BAFF mRNA level was increased in DM patients compared with normal controls. BAFF expression was markedly increased at muscle fibers in the perifascicular area but not blood vessels. BAFF-R was expressed in inflammatory cells in skeletal muscle tissues of DM patients. We found that BAFF expression in muscle tissue may be associated with an increased number of CD4 + T cells and CD19 + B cells in DM. Our study results suggest that BAFF might play an important role in the pathogenesis of DM.",
author = "Ahmi Baek and Park, {Hyung Jun} and Na, {Sang Jun} and Shim, {Dong Suk} and Moon, {Joon Shik} and Young Yang and Youngchul Choi",
year = "2012",
month = "8",
day = "15",
doi = "10.1016/j.jneuroim.2012.04.006",
language = "English",
volume = "249",
pages = "96--100",
journal = "Journal of Neuroimmunology",
issn = "0165-5728",
publisher = "Elsevier",
number = "1-2",

}

The expression of BAFF in the muscles of patients with dermatomyositis. / Baek, Ahmi; Park, Hyung Jun; Na, Sang Jun; Shim, Dong Suk; Moon, Joon Shik; Yang, Young; Choi, Youngchul.

In: Journal of Neuroimmunology, Vol. 249, No. 1-2, 15.08.2012, p. 96-100.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The expression of BAFF in the muscles of patients with dermatomyositis

AU - Baek, Ahmi

AU - Park, Hyung Jun

AU - Na, Sang Jun

AU - Shim, Dong Suk

AU - Moon, Joon Shik

AU - Yang, Young

AU - Choi, Youngchul

PY - 2012/8/15

Y1 - 2012/8/15

N2 - A B-cell activating factor of the tumor necrosis factor (TNF) family (BAFF) plays a crucial role in B-cell survival and maturation. An elevated serum BAFF level has been linked to several autoimmune diseases such as Sjögren syndrome, systemic lupus erythematosus and rheumatoid arthritis. Dermatomyositis (DM), one of autoimmune inflammatory myopathies, is characterized by inflammatory cell infiltration (CD4 + T cells and B cells) in skeletal muscle. Serum BAFF level was significantly high in DM, but the role of BAFF is not well understood. We investigated the role of BAFF in the immunopathogenesis of DM. To examine the transcriptional increase of BAFF gene expression, we performed RT-PCR analysis with skeletal muscle tissue that contained 4 controls and 9 patients with DM. Next, in order to detect BAFF expression and cellular localization in DM, we executed immunostaining in cryosection of biopsied muscle tissue with 4 controls and 8 patients and we adopted to double immunostaining to find which inflammatory cells expressed BAFF-receptor (BAFF-R). BAFF mRNA level was increased in DM patients compared with normal controls. BAFF expression was markedly increased at muscle fibers in the perifascicular area but not blood vessels. BAFF-R was expressed in inflammatory cells in skeletal muscle tissues of DM patients. We found that BAFF expression in muscle tissue may be associated with an increased number of CD4 + T cells and CD19 + B cells in DM. Our study results suggest that BAFF might play an important role in the pathogenesis of DM.

AB - A B-cell activating factor of the tumor necrosis factor (TNF) family (BAFF) plays a crucial role in B-cell survival and maturation. An elevated serum BAFF level has been linked to several autoimmune diseases such as Sjögren syndrome, systemic lupus erythematosus and rheumatoid arthritis. Dermatomyositis (DM), one of autoimmune inflammatory myopathies, is characterized by inflammatory cell infiltration (CD4 + T cells and B cells) in skeletal muscle. Serum BAFF level was significantly high in DM, but the role of BAFF is not well understood. We investigated the role of BAFF in the immunopathogenesis of DM. To examine the transcriptional increase of BAFF gene expression, we performed RT-PCR analysis with skeletal muscle tissue that contained 4 controls and 9 patients with DM. Next, in order to detect BAFF expression and cellular localization in DM, we executed immunostaining in cryosection of biopsied muscle tissue with 4 controls and 8 patients and we adopted to double immunostaining to find which inflammatory cells expressed BAFF-receptor (BAFF-R). BAFF mRNA level was increased in DM patients compared with normal controls. BAFF expression was markedly increased at muscle fibers in the perifascicular area but not blood vessels. BAFF-R was expressed in inflammatory cells in skeletal muscle tissues of DM patients. We found that BAFF expression in muscle tissue may be associated with an increased number of CD4 + T cells and CD19 + B cells in DM. Our study results suggest that BAFF might play an important role in the pathogenesis of DM.

UR - http://www.scopus.com/inward/record.url?scp=84863450303&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863450303&partnerID=8YFLogxK

U2 - 10.1016/j.jneuroim.2012.04.006

DO - 10.1016/j.jneuroim.2012.04.006

M3 - Article

C2 - 22575417

AN - SCOPUS:84863450303

VL - 249

SP - 96

EP - 100

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

SN - 0165-5728

IS - 1-2

ER -