The expression of TLR3 and cytokines induced by poly I:C in human retinal pigment epithelial cells

Ju Choi Sun, Jongwook Kim, Jung Park Su, Sook Park Hyun, Kyoung Ho Lee, Soo Ki Kim, Young Park Joo

Research output: Contribution to journalArticle

Abstract

In this study, we examined the expression of Toll-like receptor3 (TLR3) by human retinal pigment epithelial cells (RPE) and determined whether exposure to the TLR3 agonist polyinosinic-polycytidylic acid (poly I:C) would induced the expression of cytokines in these cells. RT-PCR revealed that TLR3 was constitutively expressed in human RPE, and its expression was increased by treatment with poly I:C. After treatment with poly I:C, we determined the expression levels of pro-inflammatory cytokines in human RPE using RT-PCR and ELISA. We demonstrated that poly I:C treatment increased the production of TNF-α, IL-6, and IL-8 in human RPE. Upon exposure to poly I:C, human RPE initiated antiviral response resulting in the induction of IFN-β mRNA expression and 2′,5′-oligoadenylate synthetase mRNA expression. These results suggest that human RPE may participate in ocular defense mechanism against viral infection through TLR3.

Original languageEnglish
Pages (from-to)241-248
Number of pages8
JournalJournal of Bacteriology and Virology
Volume37
Issue number4
Publication statusPublished - 2007 Jan 1

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All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Cite this

Sun, J. C., Kim, J., Su, J. P., Hyun, S. P., Lee, K. H., Kim, S. K., & Joo, Y. P. (2007). The expression of TLR3 and cytokines induced by poly I:C in human retinal pigment epithelial cells. Journal of Bacteriology and Virology, 37(4), 241-248.