The expressions of the Rb pathway in cervical intraepithelial neoplasia; predictive and prognostic significance

Eun Ji Nam, Jae Wook Kim, Sang Wun Kim, YoungTae Kim, Jae-Hoon Kim, Bo Sung Yoon, Namhoon Cho, Sunghoon Kim

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Objectives: The aim of the study was to assess the expression of the Rb pathway (p16INK4a-cdk4-pRb) in cervical intraepithelial neoplasia as a prognostic marker by investigating the association between expression of Rb pathway and the recurrence. Methods: The study group comprised of 265 paraffin-embedded tissues of the uterine cervix collected from patients between 2001 and 2003. Patients underwent ablative or excisional treatment and were followed for 2 years. RB pathway expression was examined using immunohistochemistry applied to a tissue microarray. HPV detection and genotyping were performed with HPV DNA Chip. Statistical analysis was done by ANOVA test, Student's t test, and Pearson's correlation test. Results: With increasing CIN grade, p16INK4a and cdk4 expression rates increased, while the pRb expression rate decreased. The expression rate of p16INK4a was higher (33%) in CIN I with high-risk HPV infection than in CIN I without high-risk HPV infection (19%). The mean expression rates of p16INK4a were 29%, 39%, and 64%, respectively, in CIN I, II, and III without recurrence, but 25%, 28%, and 44% in those with recurrence. The mean expression rates of pRb were 47%, 40%, and 18% in CIN I, II, and III without recurrence, respectively, but 48%, 45%, and 34% in those with recurrence. Conclusion: A relatively low expression rate of p16INK4a has prognostic significance for predicting recurrence. The clinical utility of p16INK4a status for stratifying patients according to their need for aggressive treatment requires further investigation.

Original languageEnglish
Pages (from-to)207-211
Number of pages5
JournalGynecologic Oncology
Volume104
Issue number1
DOIs
Publication statusPublished - 2007 Jan 1

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Cervical Intraepithelial Neoplasia
Recurrence
Infection
Oligonucleotide Array Sequence Analysis
Cervix Uteri
Paraffin
Analysis of Variance
Immunohistochemistry
Students
Therapeutics

All Science Journal Classification (ASJC) codes

  • Oncology
  • Obstetrics and Gynaecology

Cite this

Nam, Eun Ji ; Kim, Jae Wook ; Kim, Sang Wun ; Kim, YoungTae ; Kim, Jae-Hoon ; Yoon, Bo Sung ; Cho, Namhoon ; Kim, Sunghoon. / The expressions of the Rb pathway in cervical intraepithelial neoplasia; predictive and prognostic significance. In: Gynecologic Oncology. 2007 ; Vol. 104, No. 1. pp. 207-211.
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abstract = "Objectives: The aim of the study was to assess the expression of the Rb pathway (p16INK4a-cdk4-pRb) in cervical intraepithelial neoplasia as a prognostic marker by investigating the association between expression of Rb pathway and the recurrence. Methods: The study group comprised of 265 paraffin-embedded tissues of the uterine cervix collected from patients between 2001 and 2003. Patients underwent ablative or excisional treatment and were followed for 2 years. RB pathway expression was examined using immunohistochemistry applied to a tissue microarray. HPV detection and genotyping were performed with HPV DNA Chip. Statistical analysis was done by ANOVA test, Student's t test, and Pearson's correlation test. Results: With increasing CIN grade, p16INK4a and cdk4 expression rates increased, while the pRb expression rate decreased. The expression rate of p16INK4a was higher (33{\%}) in CIN I with high-risk HPV infection than in CIN I without high-risk HPV infection (19{\%}). The mean expression rates of p16INK4a were 29{\%}, 39{\%}, and 64{\%}, respectively, in CIN I, II, and III without recurrence, but 25{\%}, 28{\%}, and 44{\%} in those with recurrence. The mean expression rates of pRb were 47{\%}, 40{\%}, and 18{\%} in CIN I, II, and III without recurrence, respectively, but 48{\%}, 45{\%}, and 34{\%} in those with recurrence. Conclusion: A relatively low expression rate of p16INK4a has prognostic significance for predicting recurrence. The clinical utility of p16INK4a status for stratifying patients according to their need for aggressive treatment requires further investigation.",
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The expressions of the Rb pathway in cervical intraepithelial neoplasia; predictive and prognostic significance. / Nam, Eun Ji; Kim, Jae Wook; Kim, Sang Wun; Kim, YoungTae; Kim, Jae-Hoon; Yoon, Bo Sung; Cho, Namhoon; Kim, Sunghoon.

In: Gynecologic Oncology, Vol. 104, No. 1, 01.01.2007, p. 207-211.

Research output: Contribution to journalArticle

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AU - Kim, Sang Wun

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AB - Objectives: The aim of the study was to assess the expression of the Rb pathway (p16INK4a-cdk4-pRb) in cervical intraepithelial neoplasia as a prognostic marker by investigating the association between expression of Rb pathway and the recurrence. Methods: The study group comprised of 265 paraffin-embedded tissues of the uterine cervix collected from patients between 2001 and 2003. Patients underwent ablative or excisional treatment and were followed for 2 years. RB pathway expression was examined using immunohistochemistry applied to a tissue microarray. HPV detection and genotyping were performed with HPV DNA Chip. Statistical analysis was done by ANOVA test, Student's t test, and Pearson's correlation test. Results: With increasing CIN grade, p16INK4a and cdk4 expression rates increased, while the pRb expression rate decreased. The expression rate of p16INK4a was higher (33%) in CIN I with high-risk HPV infection than in CIN I without high-risk HPV infection (19%). The mean expression rates of p16INK4a were 29%, 39%, and 64%, respectively, in CIN I, II, and III without recurrence, but 25%, 28%, and 44% in those with recurrence. The mean expression rates of pRb were 47%, 40%, and 18% in CIN I, II, and III without recurrence, respectively, but 48%, 45%, and 34% in those with recurrence. Conclusion: A relatively low expression rate of p16INK4a has prognostic significance for predicting recurrence. The clinical utility of p16INK4a status for stratifying patients according to their need for aggressive treatment requires further investigation.

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