The forkhead transcription factor Foxc2 stimulates osteoblast differentiation

Se Hwa Kim, Kyoung Won Cho, Han Seok Choi, Su Jin Park, Yumie Rhee, Han Sung Jung, Sung Kil Lim

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The forkhead box C2 (Foxc2) protein is a member of the family of winged helix/forkhead transcription factors. Foxc2-deficient mice display defective formation of the aortic arches, multiple craniofacial bones, and vertebral columns. To investigate the role of Foxc2 in osteoblast differentiation, DNA containing Foxc2 was transfected into the developing cranial suture mesenchymal cells by electroporation. Compared to the controls, alkaline phosphatase (ALP) and bone sialoprotein were expressed strongly in suture mesenchymal cells in the Foxc2 overexpressed calvaria. After Foxc2-siRNA transfection, ALP staining was rarely observed in the suture mesenchyme and adjacent parietal bone of the calvaria. Meanwhile, overexpression of Foxc2 increased protein levels of β-catenin and stimulated TCF/LEF transcriptional activity. The protein kinase A inhibitor H-89 suppressed Foxc2-mediated increases in TCF/LEF transcriptional activity (-40%, P < 0.01). In conclusion, our results demonstrated that Foxc2 stimulated osteoblast differentiation of mesenchymal cells and preosteoblasts. Activation of canonical Wnt-β-catenin signals might be involved in the Foxc2-mediated stimulation of osteoblast differentiation.

Original languageEnglish
Pages (from-to)532-536
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume386
Issue number3
DOIs
Publication statusPublished - 2009 Aug 28

Fingerprint

Forkhead Transcription Factors
Osteoblasts
Catenins
Skull
Sutures
Alkaline Phosphatase
Bone
Winged-Helix Transcription Factors
Cranial Sutures
Parietal Bone
Integrin-Binding Sialoprotein
Electroporation
Arches
Mesoderm
Protein Kinase Inhibitors
Cyclic AMP-Dependent Protein Kinases
Thoracic Aorta
Small Interfering RNA
Transfection
Cell Differentiation

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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title = "The forkhead transcription factor Foxc2 stimulates osteoblast differentiation",
abstract = "The forkhead box C2 (Foxc2) protein is a member of the family of winged helix/forkhead transcription factors. Foxc2-deficient mice display defective formation of the aortic arches, multiple craniofacial bones, and vertebral columns. To investigate the role of Foxc2 in osteoblast differentiation, DNA containing Foxc2 was transfected into the developing cranial suture mesenchymal cells by electroporation. Compared to the controls, alkaline phosphatase (ALP) and bone sialoprotein were expressed strongly in suture mesenchymal cells in the Foxc2 overexpressed calvaria. After Foxc2-siRNA transfection, ALP staining was rarely observed in the suture mesenchyme and adjacent parietal bone of the calvaria. Meanwhile, overexpression of Foxc2 increased protein levels of β-catenin and stimulated TCF/LEF transcriptional activity. The protein kinase A inhibitor H-89 suppressed Foxc2-mediated increases in TCF/LEF transcriptional activity (-40{\%}, P < 0.01). In conclusion, our results demonstrated that Foxc2 stimulated osteoblast differentiation of mesenchymal cells and preosteoblasts. Activation of canonical Wnt-β-catenin signals might be involved in the Foxc2-mediated stimulation of osteoblast differentiation.",
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The forkhead transcription factor Foxc2 stimulates osteoblast differentiation. / Kim, Se Hwa; Cho, Kyoung Won; Choi, Han Seok; Park, Su Jin; Rhee, Yumie; Jung, Han Sung; Lim, Sung Kil.

In: Biochemical and Biophysical Research Communications, Vol. 386, No. 3, 28.08.2009, p. 532-536.

Research output: Contribution to journalArticle

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AU - Choi, Han Seok

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AU - Lim, Sung Kil

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