THE GENE STRUCTURE of CAENORIIABDITIS ELEGANS DNA TOPOISOMERASE I

H. S. Koo, M. H. Lee, Y. C. Kim, J. Kim

Research output: Contribution to journalArticle

Abstract

The c-DNA sequence of DNA topoisomerase I was determined using cDNA clones isolated from C. elegant cDNA libraries and also cDNA fragments obtained by RT-PCR of C elegans mRNA. The cDNA sequence is 3.2 kb long and has an open reading frame of 806 amino acids. The open reading frame has 56% identity to the amino acid sequence of human DNA topoisomerase f. The 72 kDa (> terminal polypeptide expressed in Excherichia coli cells showed caUilytic DNA topoisomerase I activity. A developmental northern analysis showed that rnRNA level of the DNA topoisomerase I was at maximum in embryos. Several genomic DNA clones of the DNA toix>isomerase I were obtained by screening a C. elegans \genomic DNA library. By analyzing the genomic DNA clones with respect to the cDNA sequence, the gene structure containing 5 exons was delineated. The pre-rnRNA of 5 exons is alternatively spliced to produce another mRNA missing the second exon. The mRNA missing the second exon was expressed at the level twice as high as the full-length mRNA in C. elegans, and relative levels of the two mRNAs did not change with development. The pre-mRNA is trans-spliced to SL1 RNA at the 5'-end as is the case with the majority of C. elegans mRNAs. The DNA topoisomerase 1 gene was mapped to position 18 of chromosome I by screening polytenc YAC plasmid DNAs.

Original languageEnglish
JournalFASEB Journal
Volume11
Issue number9
Publication statusPublished - 1997 Dec 1

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Type I DNA Topoisomerase
Messenger RNA
Exons
Complementary DNA
Clone Cells
DNA
Gene Library
Open Reading Frames
Screening
Genes
Chromosomes, Human, Pair 18
Amino Acids
Isomerases
Genomic Library
DNA sequences
RNA Precursors
Chromosomes
Amino Acid Sequence
Plasmids
Embryonic Structures

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Koo, H. S. ; Lee, M. H. ; Kim, Y. C. ; Kim, J. / THE GENE STRUCTURE of CAENORIIABDITIS ELEGANS DNA TOPOISOMERASE I. In: FASEB Journal. 1997 ; Vol. 11, No. 9.
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THE GENE STRUCTURE of CAENORIIABDITIS ELEGANS DNA TOPOISOMERASE I. / Koo, H. S.; Lee, M. H.; Kim, Y. C.; Kim, J.

In: FASEB Journal, Vol. 11, No. 9, 01.12.1997.

Research output: Contribution to journalArticle

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