The genome-wide expression profile of gastric epithelial cells infected by naturally occurring cagA isogenic strains of Helicobacter pylori

Sung Hwa Sohn, Yongchan Lee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Helicobacter pylori (H. pylori) is associated with the development of gastric adenocarcinoma and lymphoma. However, the mechanisms through which H. pylori induces gastric mucosal lesions are not well defined. This study was conducted to evaluate the effect of the oncoprotein CagA on gastric cancer cells using whole-genome expression arrays. Human gastric epithelial (AGS) cells were incubated with CagA-positive H. pylori strains (147C (phosphorylated CagA) or 147A (dephosphorylated CagA)), and total protein and RNA were collected. The effects of phosphorylated and unphosphorylated CagA on AGS cells were then evaluated using Western blotting and microarray analysis. The expression levels of the genome profiles of AGS cells infected with 147A were compared with those of AGS cells infected with 147C. The expression profiles of the differentially expressed genes were grouped, and their expression patterns were validated via quantitative real-time PCR. Up- and down-regulated genes mainly included epithelial mesenchymal transition (EMT)-related genes. The results of the microarray analysis revealed that phosphorylated and unphosphorylated CagA may affect EMT in part through gene expression. This suggests that the intracellularly translocated CagA may be involved in EMT, resulting in differential expression of genes independent on the phosphorylation status of CagA.

Original languageEnglish
Pages (from-to)382-389
Number of pages8
JournalEnvironmental Toxicology and Pharmacology
Volume32
Issue number3
DOIs
Publication statusPublished - 2011 Nov 1

Fingerprint

Helicobacter pylori
Epithelial-Mesenchymal Transition
Stomach
Genes
Epithelial Cells
Genome
Microarray Analysis
Microarrays
Gene Expression
Oncogene Proteins
Stomach Neoplasms
Real-Time Polymerase Chain Reaction
Phosphorylation
Adenocarcinoma
Western Blotting
RNA
Gene expression
Cells
Proteins

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis

Cite this

@article{6360f0e8dfc446628cdafbb03880e0af,
title = "The genome-wide expression profile of gastric epithelial cells infected by naturally occurring cagA isogenic strains of Helicobacter pylori",
abstract = "Helicobacter pylori (H. pylori) is associated with the development of gastric adenocarcinoma and lymphoma. However, the mechanisms through which H. pylori induces gastric mucosal lesions are not well defined. This study was conducted to evaluate the effect of the oncoprotein CagA on gastric cancer cells using whole-genome expression arrays. Human gastric epithelial (AGS) cells were incubated with CagA-positive H. pylori strains (147C (phosphorylated CagA) or 147A (dephosphorylated CagA)), and total protein and RNA were collected. The effects of phosphorylated and unphosphorylated CagA on AGS cells were then evaluated using Western blotting and microarray analysis. The expression levels of the genome profiles of AGS cells infected with 147A were compared with those of AGS cells infected with 147C. The expression profiles of the differentially expressed genes were grouped, and their expression patterns were validated via quantitative real-time PCR. Up- and down-regulated genes mainly included epithelial mesenchymal transition (EMT)-related genes. The results of the microarray analysis revealed that phosphorylated and unphosphorylated CagA may affect EMT in part through gene expression. This suggests that the intracellularly translocated CagA may be involved in EMT, resulting in differential expression of genes independent on the phosphorylation status of CagA.",
author = "Sohn, {Sung Hwa} and Yongchan Lee",
year = "2011",
month = "11",
day = "1",
doi = "10.1016/j.etap.2011.08.006",
language = "English",
volume = "32",
pages = "382--389",
journal = "Environmental Toxicology and Pharmacology",
issn = "1382-6689",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - The genome-wide expression profile of gastric epithelial cells infected by naturally occurring cagA isogenic strains of Helicobacter pylori

AU - Sohn, Sung Hwa

AU - Lee, Yongchan

PY - 2011/11/1

Y1 - 2011/11/1

N2 - Helicobacter pylori (H. pylori) is associated with the development of gastric adenocarcinoma and lymphoma. However, the mechanisms through which H. pylori induces gastric mucosal lesions are not well defined. This study was conducted to evaluate the effect of the oncoprotein CagA on gastric cancer cells using whole-genome expression arrays. Human gastric epithelial (AGS) cells were incubated with CagA-positive H. pylori strains (147C (phosphorylated CagA) or 147A (dephosphorylated CagA)), and total protein and RNA were collected. The effects of phosphorylated and unphosphorylated CagA on AGS cells were then evaluated using Western blotting and microarray analysis. The expression levels of the genome profiles of AGS cells infected with 147A were compared with those of AGS cells infected with 147C. The expression profiles of the differentially expressed genes were grouped, and their expression patterns were validated via quantitative real-time PCR. Up- and down-regulated genes mainly included epithelial mesenchymal transition (EMT)-related genes. The results of the microarray analysis revealed that phosphorylated and unphosphorylated CagA may affect EMT in part through gene expression. This suggests that the intracellularly translocated CagA may be involved in EMT, resulting in differential expression of genes independent on the phosphorylation status of CagA.

AB - Helicobacter pylori (H. pylori) is associated with the development of gastric adenocarcinoma and lymphoma. However, the mechanisms through which H. pylori induces gastric mucosal lesions are not well defined. This study was conducted to evaluate the effect of the oncoprotein CagA on gastric cancer cells using whole-genome expression arrays. Human gastric epithelial (AGS) cells were incubated with CagA-positive H. pylori strains (147C (phosphorylated CagA) or 147A (dephosphorylated CagA)), and total protein and RNA were collected. The effects of phosphorylated and unphosphorylated CagA on AGS cells were then evaluated using Western blotting and microarray analysis. The expression levels of the genome profiles of AGS cells infected with 147A were compared with those of AGS cells infected with 147C. The expression profiles of the differentially expressed genes were grouped, and their expression patterns were validated via quantitative real-time PCR. Up- and down-regulated genes mainly included epithelial mesenchymal transition (EMT)-related genes. The results of the microarray analysis revealed that phosphorylated and unphosphorylated CagA may affect EMT in part through gene expression. This suggests that the intracellularly translocated CagA may be involved in EMT, resulting in differential expression of genes independent on the phosphorylation status of CagA.

UR - http://www.scopus.com/inward/record.url?scp=80054105102&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80054105102&partnerID=8YFLogxK

U2 - 10.1016/j.etap.2011.08.006

DO - 10.1016/j.etap.2011.08.006

M3 - Article

VL - 32

SP - 382

EP - 389

JO - Environmental Toxicology and Pharmacology

JF - Environmental Toxicology and Pharmacology

SN - 1382-6689

IS - 3

ER -