Helicobacter pylori (H. pylori) is associated with the development of gastric adenocarcinoma and lymphoma. However, the mechanisms through which H. pylori induces gastric mucosal lesions are not well defined. This study was conducted to evaluate the effect of the oncoprotein CagA on gastric cancer cells using whole-genome expression arrays. Human gastric epithelial (AGS) cells were incubated with CagA-positive H. pylori strains (147C (phosphorylated CagA) or 147A (dephosphorylated CagA)), and total protein and RNA were collected. The effects of phosphorylated and unphosphorylated CagA on AGS cells were then evaluated using Western blotting and microarray analysis. The expression levels of the genome profiles of AGS cells infected with 147A were compared with those of AGS cells infected with 147C. The expression profiles of the differentially expressed genes were grouped, and their expression patterns were validated via quantitative real-time PCR. Up- and down-regulated genes mainly included epithelial mesenchymal transition (EMT)-related genes. The results of the microarray analysis revealed that phosphorylated and unphosphorylated CagA may affect EMT in part through gene expression. This suggests that the intracellularly translocated CagA may be involved in EMT, resulting in differential expression of genes independent on the phosphorylation status of CagA.
Bibliographical noteFunding Information:
This study was supported by a grant from the Korea Healthcare Technology R&D project, Ministry for Health, Welfare & Family Affairs , Republic of Korea ( A100238 ). M. Blaser has a potential royalty interest in CagA diagnostics (licensed by Vanderbilt University) based on his discovery of CagA.
All Science Journal Classification (ASJC) codes
- Health, Toxicology and Mutagenesis