The genome-wide expression profile of Nelumbinis semen on lipopolysaccharide-stimulated BV-2 microglial cells

Sung Hwa Sohn, Hwan Suck Chung, Eunjung Ko, Hyuk Joon Jeong, Sung Hoon Kim, Jin-Hyun Jeong, Yangseok Kim, Minkyu Shin, Moochang Hong, Hyunsu Bae

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

This study was conducted to evaluate the protective mechanisms of Nelumbinis semen (NS) on lipopolysaccharide (LPS)-induced activation of BV-2 microglial cells. The anti-inflammatory effects of NS were determined by analyzing nitric oxide production and proinflammatory cytokines using enzyme-linked immunosorbent assay. The mechanism was evaluated in BV-2 cells with or without NS treated with LPS for various lengths of time using oligonucleotide microarray and real time reverse transcription-polymerase chain reaction. The oligonucleotide microarray analysis revealed that mitogen activated protein kinase (MAPK) signaling pathway-related genes such as Fgfr3, Fgf12, Rasal2, Nfkb2, Map2k5, Mapk1, Map3k7, and NFatc2 were down-regulated in LPS activated BV-2 cells by pretreatment with NS. In addition, significant decreases in Nos1ap gene expression were observed with NS pretreatment. Cluster linked pathway analysis using the Kyoto Encyclopedia of Genes and Genomes database revealed that the effects of NS were closely associated with the regulation of mitochondria functions. These results suggested that NS can affect the MAPK signaling pathway and mitochondrial functions in BV-2 cells activated with LPS.

Original languageEnglish
Pages (from-to)1012-1020
Number of pages9
JournalBiological and Pharmaceutical Bulletin
Volume32
Issue number6
DOIs
Publication statusPublished - 2009 Jun 1

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Lipopolysaccharides
Genome
Mitogen-Activated Protein Kinases
Oligonucleotide Array Sequence Analysis
Encyclopedias
Microarray Analysis
Nelumbinis Semen
Genes
Reverse Transcription
Mitochondria
Nitric Oxide
Anti-Inflammatory Agents
Enzyme-Linked Immunosorbent Assay
Databases
Cytokines
Gene Expression
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

Sohn, Sung Hwa ; Chung, Hwan Suck ; Ko, Eunjung ; Jeong, Hyuk Joon ; Kim, Sung Hoon ; Jeong, Jin-Hyun ; Kim, Yangseok ; Shin, Minkyu ; Hong, Moochang ; Bae, Hyunsu. / The genome-wide expression profile of Nelumbinis semen on lipopolysaccharide-stimulated BV-2 microglial cells. In: Biological and Pharmaceutical Bulletin. 2009 ; Vol. 32, No. 6. pp. 1012-1020.
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The genome-wide expression profile of Nelumbinis semen on lipopolysaccharide-stimulated BV-2 microglial cells. / Sohn, Sung Hwa; Chung, Hwan Suck; Ko, Eunjung; Jeong, Hyuk Joon; Kim, Sung Hoon; Jeong, Jin-Hyun; Kim, Yangseok; Shin, Minkyu; Hong, Moochang; Bae, Hyunsu.

In: Biological and Pharmaceutical Bulletin, Vol. 32, No. 6, 01.06.2009, p. 1012-1020.

Research output: Contribution to journalArticle

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