The Hrp pathogenicity island of Erwinia amylovora and identification of three novel genes required for systemic infection

Chang Sik Oh, Jihyun F. Kim, Steven V. Beer

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Sequence analysis of the region bordering the hrp/dsp gene cluster of Erwinia amylovora strain Ea321, which causes fire blight, revealed characteristics of pathogenicity islands (PAIs). Included are genes for a phage integrase, a tRNAPhe, several orthologues of genes of YAPI, a PAI of Yersinia pseudotuberculosis, and several putative virulence genes with HrpL-dependent promoter motifs. The island is designated the Hrp PAI of E. amylovora. It is comprised of a chromosomal region of c. 62 kb with 60 open reading frames (ORFs). Comparison of the Hrp PAI of E. amylovora with those of four closely related bacteria showed that orfB, a homologue of avrBsT of Xanthomonas campestris pv. vesicatoria, and orfA, its putative chaperone gene, are present only in the Hrp PAI of E. amylovora. As regions flanking the hrp/dsp gene cluster are quite diverse, addition and deletion may have occurred during divergent evolution of the five bacteria. Among ORFs of the PAI of Ea321, three new HrpL-dependent genes were identified. Because they are required for full virulence in apple, they were designated hsvC, hsvB and hsvA (hrp-associated systemic virulence). They encode a homologue of an amidinotransferase for phaseolotoxin biosynthesis and homologues of a nikkomycin-biosynthetic protein of Pseudomonas syringae.

Original languageEnglish
Pages (from-to)125-138
Number of pages14
JournalMolecular Plant Pathology
Volume6
Issue number2
DOIs
Publication statusPublished - 2005 Mar 1

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Erwinia amylovora
pathogenicity islands
Genomic Islands
pathogenicity
gene
Infection
infection
Genes
Virulence
virulence
genes
Multigene Family
multigene family
Open Reading Frames
amidinotransferases
open reading frames
nikkomycins
phaseolotoxin
Amidinotransferases
Xanthomonas vesicatoria

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

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title = "The Hrp pathogenicity island of Erwinia amylovora and identification of three novel genes required for systemic infection",
abstract = "Sequence analysis of the region bordering the hrp/dsp gene cluster of Erwinia amylovora strain Ea321, which causes fire blight, revealed characteristics of pathogenicity islands (PAIs). Included are genes for a phage integrase, a tRNAPhe, several orthologues of genes of YAPI, a PAI of Yersinia pseudotuberculosis, and several putative virulence genes with HrpL-dependent promoter motifs. The island is designated the Hrp PAI of E. amylovora. It is comprised of a chromosomal region of c. 62 kb with 60 open reading frames (ORFs). Comparison of the Hrp PAI of E. amylovora with those of four closely related bacteria showed that orfB, a homologue of avrBsT of Xanthomonas campestris pv. vesicatoria, and orfA, its putative chaperone gene, are present only in the Hrp PAI of E. amylovora. As regions flanking the hrp/dsp gene cluster are quite diverse, addition and deletion may have occurred during divergent evolution of the five bacteria. Among ORFs of the PAI of Ea321, three new HrpL-dependent genes were identified. Because they are required for full virulence in apple, they were designated hsvC, hsvB and hsvA (hrp-associated systemic virulence). They encode a homologue of an amidinotransferase for phaseolotoxin biosynthesis and homologues of a nikkomycin-biosynthetic protein of Pseudomonas syringae.",
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The Hrp pathogenicity island of Erwinia amylovora and identification of three novel genes required for systemic infection. / Oh, Chang Sik; Kim, Jihyun F.; Beer, Steven V.

In: Molecular Plant Pathology, Vol. 6, No. 2, 01.03.2005, p. 125-138.

Research output: Contribution to journalArticle

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