The human PTFγ/SNAP43 gene: Structure, chromosomal location, and identification of a VNTR in 5'-UTR

Jee Hye Maeng, Jong-Bok Yoon

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

PTF/SNAPc is a multisubunit complex which specifically recognizes the PSEs of small nuclear RNA genes and activates transcription by RNA polymerase II or III. Here we describe the isolation and characterization of genomic clones encoding the human PTFγ/SNAP43 gene. The gene spans approximately 29 kilobases, and is composed of 9 exons and 8 introns. A major transcription initiation site was identified at the position 58 base pairs upstream of the AUG translation initiator codon on primer extension analysis with HeLa mRNA. The 5' flanking region lacks a typical TATA box but contains many putative binding sites for various transcription factors, such as Sp1, Oct1, NF1, AP1, E2F, and USF. Immediately downstream of the transcription start site, we found a VNTR of a 17-bp sequence rich in (G+C). Four different alleles with two to five copies of the tandem repeat were identified in 10 individuals examined, indicating a high degree of variation at the PTFγ/SNAP43 locus. In addition, the PTFγ/SNAP43 gene was mapped to human chromosome 14q22 by fluorescence in situ hybridization.

Original languageEnglish
Pages (from-to)23-27
Number of pages5
JournalJournal of Biochemistry
Volume124
Issue number1
DOIs
Publication statusPublished - 1998 Jan 1

Fingerprint

5' Untranslated Regions
Genes
Transcription Initiation Site
RNA Polymerase III
Small Nuclear RNA
TATA Box
Tandem Repeat Sequences
Initiator Codon
RNA Polymerase II
5' Flanking Region
Human Chromosomes
Transcription
Chromosomes
Fluorescence In Situ Hybridization
Base Pairing
Introns
Exons
Transcription Factors
Clone Cells
Fluorescence

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

@article{4ce6803d57bf4044bea1c5356ff882c8,
title = "The human PTFγ/SNAP43 gene: Structure, chromosomal location, and identification of a VNTR in 5'-UTR",
abstract = "PTF/SNAPc is a multisubunit complex which specifically recognizes the PSEs of small nuclear RNA genes and activates transcription by RNA polymerase II or III. Here we describe the isolation and characterization of genomic clones encoding the human PTFγ/SNAP43 gene. The gene spans approximately 29 kilobases, and is composed of 9 exons and 8 introns. A major transcription initiation site was identified at the position 58 base pairs upstream of the AUG translation initiator codon on primer extension analysis with HeLa mRNA. The 5' flanking region lacks a typical TATA box but contains many putative binding sites for various transcription factors, such as Sp1, Oct1, NF1, AP1, E2F, and USF. Immediately downstream of the transcription start site, we found a VNTR of a 17-bp sequence rich in (G+C). Four different alleles with two to five copies of the tandem repeat were identified in 10 individuals examined, indicating a high degree of variation at the PTFγ/SNAP43 locus. In addition, the PTFγ/SNAP43 gene was mapped to human chromosome 14q22 by fluorescence in situ hybridization.",
author = "Maeng, {Jee Hye} and Jong-Bok Yoon",
year = "1998",
month = "1",
day = "1",
doi = "10.1093/oxfordjournals.jbchem.a022085",
language = "English",
volume = "124",
pages = "23--27",
journal = "Journal of Biochemistry",
issn = "0021-924X",
publisher = "Oxford University Press",
number = "1",

}

The human PTFγ/SNAP43 gene : Structure, chromosomal location, and identification of a VNTR in 5'-UTR. / Maeng, Jee Hye; Yoon, Jong-Bok.

In: Journal of Biochemistry, Vol. 124, No. 1, 01.01.1998, p. 23-27.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The human PTFγ/SNAP43 gene

T2 - Structure, chromosomal location, and identification of a VNTR in 5'-UTR

AU - Maeng, Jee Hye

AU - Yoon, Jong-Bok

PY - 1998/1/1

Y1 - 1998/1/1

N2 - PTF/SNAPc is a multisubunit complex which specifically recognizes the PSEs of small nuclear RNA genes and activates transcription by RNA polymerase II or III. Here we describe the isolation and characterization of genomic clones encoding the human PTFγ/SNAP43 gene. The gene spans approximately 29 kilobases, and is composed of 9 exons and 8 introns. A major transcription initiation site was identified at the position 58 base pairs upstream of the AUG translation initiator codon on primer extension analysis with HeLa mRNA. The 5' flanking region lacks a typical TATA box but contains many putative binding sites for various transcription factors, such as Sp1, Oct1, NF1, AP1, E2F, and USF. Immediately downstream of the transcription start site, we found a VNTR of a 17-bp sequence rich in (G+C). Four different alleles with two to five copies of the tandem repeat were identified in 10 individuals examined, indicating a high degree of variation at the PTFγ/SNAP43 locus. In addition, the PTFγ/SNAP43 gene was mapped to human chromosome 14q22 by fluorescence in situ hybridization.

AB - PTF/SNAPc is a multisubunit complex which specifically recognizes the PSEs of small nuclear RNA genes and activates transcription by RNA polymerase II or III. Here we describe the isolation and characterization of genomic clones encoding the human PTFγ/SNAP43 gene. The gene spans approximately 29 kilobases, and is composed of 9 exons and 8 introns. A major transcription initiation site was identified at the position 58 base pairs upstream of the AUG translation initiator codon on primer extension analysis with HeLa mRNA. The 5' flanking region lacks a typical TATA box but contains many putative binding sites for various transcription factors, such as Sp1, Oct1, NF1, AP1, E2F, and USF. Immediately downstream of the transcription start site, we found a VNTR of a 17-bp sequence rich in (G+C). Four different alleles with two to five copies of the tandem repeat were identified in 10 individuals examined, indicating a high degree of variation at the PTFγ/SNAP43 locus. In addition, the PTFγ/SNAP43 gene was mapped to human chromosome 14q22 by fluorescence in situ hybridization.

UR - http://www.scopus.com/inward/record.url?scp=0031824190&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031824190&partnerID=8YFLogxK

U2 - 10.1093/oxfordjournals.jbchem.a022085

DO - 10.1093/oxfordjournals.jbchem.a022085

M3 - Article

C2 - 9644240

AN - SCOPUS:0031824190

VL - 124

SP - 23

EP - 27

JO - Journal of Biochemistry

JF - Journal of Biochemistry

SN - 0021-924X

IS - 1

ER -