The immunity and protective effects of antigen 85A and heat-shock protein X against progressive tuberculosis

Bo Young Jeon, Seung Cheol Kim, Seok Yong Eum, Sang Nae Cho

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The anti-tuberculosis vaccine, Mycobacterium bovis BCG, has been used worldwide, but its protective efficacy is variable against adult pulmonary tuberculosis. In this study, immune responses of antigen 85A (Ag85A) and heat-shock protein X (HspX) antigen of Mycobacterium tuberculosis were investigated during acute and stationary stage of infection in the murine aerosol TB challenge model and their protective effects were evaluated against progressive tuberculosis. A high level of Ag85A-specific IFN-γ production was induced from the early stage of the infection, whereas HspX-specific IFN-γ production was increased in the later stationary stage. As a subunit vaccine, Ag85A and HspX antigen vaccine induced high levels of IFN-γ, and a vaccine comprising both antigens induced the highest level of IFN-γ. At 30 days post-challenge, the Ag85A subunit vaccine was protective against M. tuberculosis challenge, but the HspX subunit vaccine was not. Interestingly, the HspX antigen vaccine induced significant protective efficacy at 90 days post-challenge. Moreover, the combined antigen vaccine induced the highest protective efficacy against M. tuberculosis challenge both at 30 days and 90 days post-challenge. These results suggest that the vaccine comprising Ag85A and HspX antigen which react in different stages of infection is highly protective against progressive tuberculosis.

Original languageEnglish
Pages (from-to)284-290
Number of pages7
JournalMicrobes and Infection
Volume13
Issue number3
DOIs
Publication statusPublished - 2011 Mar 1

Fingerprint

Heat-Shock Proteins
Immunity
Tuberculosis
Antigens
Subunit Vaccines
Vaccines
Mycobacterium bovis
Mycobacterium tuberculosis
Infection
Tuberculosis Vaccines
Combined Vaccines
Histocompatibility Antigens Class II
Protein Subunits
Aerosols
Pulmonary Tuberculosis

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

@article{75fb26d27c2e406a873c72a11e114855,
title = "The immunity and protective effects of antigen 85A and heat-shock protein X against progressive tuberculosis",
abstract = "The anti-tuberculosis vaccine, Mycobacterium bovis BCG, has been used worldwide, but its protective efficacy is variable against adult pulmonary tuberculosis. In this study, immune responses of antigen 85A (Ag85A) and heat-shock protein X (HspX) antigen of Mycobacterium tuberculosis were investigated during acute and stationary stage of infection in the murine aerosol TB challenge model and their protective effects were evaluated against progressive tuberculosis. A high level of Ag85A-specific IFN-γ production was induced from the early stage of the infection, whereas HspX-specific IFN-γ production was increased in the later stationary stage. As a subunit vaccine, Ag85A and HspX antigen vaccine induced high levels of IFN-γ, and a vaccine comprising both antigens induced the highest level of IFN-γ. At 30 days post-challenge, the Ag85A subunit vaccine was protective against M. tuberculosis challenge, but the HspX subunit vaccine was not. Interestingly, the HspX antigen vaccine induced significant protective efficacy at 90 days post-challenge. Moreover, the combined antigen vaccine induced the highest protective efficacy against M. tuberculosis challenge both at 30 days and 90 days post-challenge. These results suggest that the vaccine comprising Ag85A and HspX antigen which react in different stages of infection is highly protective against progressive tuberculosis.",
author = "Jeon, {Bo Young} and Kim, {Seung Cheol} and Eum, {Seok Yong} and Cho, {Sang Nae}",
year = "2011",
month = "3",
day = "1",
doi = "10.1016/j.micinf.2010.11.002",
language = "English",
volume = "13",
pages = "284--290",
journal = "Microbes and Infection",
issn = "1286-4579",
publisher = "Elsevier Masson SAS",
number = "3",

}

The immunity and protective effects of antigen 85A and heat-shock protein X against progressive tuberculosis. / Jeon, Bo Young; Kim, Seung Cheol; Eum, Seok Yong; Cho, Sang Nae.

In: Microbes and Infection, Vol. 13, No. 3, 01.03.2011, p. 284-290.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The immunity and protective effects of antigen 85A and heat-shock protein X against progressive tuberculosis

AU - Jeon, Bo Young

AU - Kim, Seung Cheol

AU - Eum, Seok Yong

AU - Cho, Sang Nae

PY - 2011/3/1

Y1 - 2011/3/1

N2 - The anti-tuberculosis vaccine, Mycobacterium bovis BCG, has been used worldwide, but its protective efficacy is variable against adult pulmonary tuberculosis. In this study, immune responses of antigen 85A (Ag85A) and heat-shock protein X (HspX) antigen of Mycobacterium tuberculosis were investigated during acute and stationary stage of infection in the murine aerosol TB challenge model and their protective effects were evaluated against progressive tuberculosis. A high level of Ag85A-specific IFN-γ production was induced from the early stage of the infection, whereas HspX-specific IFN-γ production was increased in the later stationary stage. As a subunit vaccine, Ag85A and HspX antigen vaccine induced high levels of IFN-γ, and a vaccine comprising both antigens induced the highest level of IFN-γ. At 30 days post-challenge, the Ag85A subunit vaccine was protective against M. tuberculosis challenge, but the HspX subunit vaccine was not. Interestingly, the HspX antigen vaccine induced significant protective efficacy at 90 days post-challenge. Moreover, the combined antigen vaccine induced the highest protective efficacy against M. tuberculosis challenge both at 30 days and 90 days post-challenge. These results suggest that the vaccine comprising Ag85A and HspX antigen which react in different stages of infection is highly protective against progressive tuberculosis.

AB - The anti-tuberculosis vaccine, Mycobacterium bovis BCG, has been used worldwide, but its protective efficacy is variable against adult pulmonary tuberculosis. In this study, immune responses of antigen 85A (Ag85A) and heat-shock protein X (HspX) antigen of Mycobacterium tuberculosis were investigated during acute and stationary stage of infection in the murine aerosol TB challenge model and their protective effects were evaluated against progressive tuberculosis. A high level of Ag85A-specific IFN-γ production was induced from the early stage of the infection, whereas HspX-specific IFN-γ production was increased in the later stationary stage. As a subunit vaccine, Ag85A and HspX antigen vaccine induced high levels of IFN-γ, and a vaccine comprising both antigens induced the highest level of IFN-γ. At 30 days post-challenge, the Ag85A subunit vaccine was protective against M. tuberculosis challenge, but the HspX subunit vaccine was not. Interestingly, the HspX antigen vaccine induced significant protective efficacy at 90 days post-challenge. Moreover, the combined antigen vaccine induced the highest protective efficacy against M. tuberculosis challenge both at 30 days and 90 days post-challenge. These results suggest that the vaccine comprising Ag85A and HspX antigen which react in different stages of infection is highly protective against progressive tuberculosis.

UR - http://www.scopus.com/inward/record.url?scp=78951493314&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78951493314&partnerID=8YFLogxK

U2 - 10.1016/j.micinf.2010.11.002

DO - 10.1016/j.micinf.2010.11.002

M3 - Article

C2 - 21093603

AN - SCOPUS:78951493314

VL - 13

SP - 284

EP - 290

JO - Microbes and Infection

JF - Microbes and Infection

SN - 1286-4579

IS - 3

ER -