The immunological and oxidative stress regulation of non-thermal plasma-aided water on atopic dermatitis-like lesion in dinitrochlorobenzene-induced SKH-1 hairless mice

Jesmin Ara, Johny Bajgai, Ma Easter Joy Sajo, Ailyn Fadriquela, Cheol Su Kim, Soo Ki Kim, Kyu Jae Lee

Research output: Contribution to journalArticle

Abstract

Backgrounds: While non-thermal plasma aided water (NTP-AW) has been currently used as a novel therapeutic against wide array of diseases, it remains unknown on therapeutic effect against atopic dermatitis (AD). To answer this, we evaluated the effect of NTP-AW on the regulation of immune and antioxidant markers in dinitrochlorobenzene (DNCB)-induced AD in hairless mice. Methods: The skin damage in mice was induced by 1% DNCB for the first wk and 0.5% every alternate day for 3 wks followed by 4 wks NTP-AW treatment through spraying and bathing method. Results: Treatment with NTP-AW reduced the severity of dermatitis symptoms and skin scratching behavior. In line, immunological profiling showed the improved parameters such as decrease in the number of neutrophils, lymphocyte, eosinophil as well as Th2-mediated IgE overproduction, and the reduced level of pro-inflammatory cytokine such as interleukin (IL)-1β, and tumor necrosis factor (TNF)-α. Redox profiling showed that NTP-AW reduced the production of total reactive oxygen species (ROS), and enhanced the antioxidant enzyme activity. Conclusion: Collectively, this study clearly indicates the therapeutic effect of NTP-AW on DNCB-induced AD in hairless mice via immuno-redox regulation, clinically implying that NTP-AW treatment might be a safe and promising therapeutic candidate against AD.

Original languageEnglish
Pages (from-to)199-208
Number of pages10
JournalMolecular and Cellular Toxicology
Volume15
Issue number2
DOIs
Publication statusPublished - 2019 Apr 1

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Hairless Mouse
Dinitrochlorobenzene
Oxidative stress
Atopic Dermatitis
Oxidative Stress
Plasmas
Water
Water Purification
Therapeutic Uses
Water treatment
Oxidation-Reduction
Skin
Antioxidants
Dermatitis
Lymphocytes
Lymphocyte Count
Enzyme activity
Spraying
Interleukin-1
Eosinophils

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Toxicology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Ara, Jesmin ; Bajgai, Johny ; Sajo, Ma Easter Joy ; Fadriquela, Ailyn ; Kim, Cheol Su ; Kim, Soo Ki ; Lee, Kyu Jae. / The immunological and oxidative stress regulation of non-thermal plasma-aided water on atopic dermatitis-like lesion in dinitrochlorobenzene-induced SKH-1 hairless mice. In: Molecular and Cellular Toxicology. 2019 ; Vol. 15, No. 2. pp. 199-208.
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abstract = "Backgrounds: While non-thermal plasma aided water (NTP-AW) has been currently used as a novel therapeutic against wide array of diseases, it remains unknown on therapeutic effect against atopic dermatitis (AD). To answer this, we evaluated the effect of NTP-AW on the regulation of immune and antioxidant markers in dinitrochlorobenzene (DNCB)-induced AD in hairless mice. Methods: The skin damage in mice was induced by 1{\%} DNCB for the first wk and 0.5{\%} every alternate day for 3 wks followed by 4 wks NTP-AW treatment through spraying and bathing method. Results: Treatment with NTP-AW reduced the severity of dermatitis symptoms and skin scratching behavior. In line, immunological profiling showed the improved parameters such as decrease in the number of neutrophils, lymphocyte, eosinophil as well as Th2-mediated IgE overproduction, and the reduced level of pro-inflammatory cytokine such as interleukin (IL)-1β, and tumor necrosis factor (TNF)-α. Redox profiling showed that NTP-AW reduced the production of total reactive oxygen species (ROS), and enhanced the antioxidant enzyme activity. Conclusion: Collectively, this study clearly indicates the therapeutic effect of NTP-AW on DNCB-induced AD in hairless mice via immuno-redox regulation, clinically implying that NTP-AW treatment might be a safe and promising therapeutic candidate against AD.",
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The immunological and oxidative stress regulation of non-thermal plasma-aided water on atopic dermatitis-like lesion in dinitrochlorobenzene-induced SKH-1 hairless mice. / Ara, Jesmin; Bajgai, Johny; Sajo, Ma Easter Joy; Fadriquela, Ailyn; Kim, Cheol Su; Kim, Soo Ki; Lee, Kyu Jae.

In: Molecular and Cellular Toxicology, Vol. 15, No. 2, 01.04.2019, p. 199-208.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The immunological and oxidative stress regulation of non-thermal plasma-aided water on atopic dermatitis-like lesion in dinitrochlorobenzene-induced SKH-1 hairless mice

AU - Ara, Jesmin

AU - Bajgai, Johny

AU - Sajo, Ma Easter Joy

AU - Fadriquela, Ailyn

AU - Kim, Cheol Su

AU - Kim, Soo Ki

AU - Lee, Kyu Jae

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