The influence of lithium fluoride on in vitro biocompatibility and bioactivity of calcium aluminate-PMMA composite cement

S. H. Oh, S. Y. Choi, Seongho Choi, Y. K. Lee, K. N. Kim

Research output: Contribution to journalArticle

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Abstract

The objective of this study is to assess the influence of lithium fluoride on in vitro biocompatibility and bioactivity of calcium aluminate (CA)-polymethylmethacrylate (PMMA) composite cement exhibiting quick setting time ( < 15 min), low exothermic temperature ( < 47°C), and high compressive strength ( > 100 MPa). The biocompatibility was measured by examining cytotoxicity tests such as the agar diffusion test with L929 cell line and the hemolysis test with fresh rabbit blood. To estimate the bioactivity of CA-PMMA composite cement, we determined hydroxyapatite (HAp) formation on the surface of composite cement in the simulated body (SBF) solution by using thin-film XRD, XPS, SEM, EPMA and ICP-AES. The results of biocompatibility tests indicated that all experimental compositions of this study had no cytotoxicity and no hemolysis so that there was no cytotoxicity with regard to non-reacted monomers (MMA and TEGDMA) and lithium fluoride. The results of bioactivity tests revealed that CA-PMMA composite cement without lithium fluoride did not form HAp on its surface after 60 days of soaking in the SBF. On the other hand, LiAl2(OH)7·2H 2O and HAp were formed on the surface of CA-PMMA composite cement including 1.0% by weight of lithium fluoride after 7 and 15 days of soaking in the SBF, respectively. The 5 μm of LiAl2(OH)7 ·2H2O and HAp mixed layers were formed on the surface of specimen after 60 days of soaking in the SBF.

Original languageEnglish
Pages (from-to)25-33
Number of pages9
JournalJournal of Materials Science: Materials in Medicine
Volume15
Issue number1
DOIs
Publication statusPublished - 2004 Jan 1

Fingerprint

Polymethyl Methacrylate
Bioactivity
Biocompatibility
Calcium
Cements
Lithium
Hydroxyapatite
Durapatite
Cytotoxicity
Composite materials
Hemolysis
Bone cement
Forms (concrete)
Agar
Electron probe microanalysis
Hand
Rabbits
Weights and Measures
Cell Line
Blood

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

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abstract = "The objective of this study is to assess the influence of lithium fluoride on in vitro biocompatibility and bioactivity of calcium aluminate (CA)-polymethylmethacrylate (PMMA) composite cement exhibiting quick setting time ( < 15 min), low exothermic temperature ( < 47°C), and high compressive strength ( > 100 MPa). The biocompatibility was measured by examining cytotoxicity tests such as the agar diffusion test with L929 cell line and the hemolysis test with fresh rabbit blood. To estimate the bioactivity of CA-PMMA composite cement, we determined hydroxyapatite (HAp) formation on the surface of composite cement in the simulated body (SBF) solution by using thin-film XRD, XPS, SEM, EPMA and ICP-AES. The results of biocompatibility tests indicated that all experimental compositions of this study had no cytotoxicity and no hemolysis so that there was no cytotoxicity with regard to non-reacted monomers (MMA and TEGDMA) and lithium fluoride. The results of bioactivity tests revealed that CA-PMMA composite cement without lithium fluoride did not form HAp on its surface after 60 days of soaking in the SBF. On the other hand, LiAl2(OH)7·2H 2O and HAp were formed on the surface of CA-PMMA composite cement including 1.0{\%} by weight of lithium fluoride after 7 and 15 days of soaking in the SBF, respectively. The 5 μm of LiAl2(OH)7 ·2H2O and HAp mixed layers were formed on the surface of specimen after 60 days of soaking in the SBF.",
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The influence of lithium fluoride on in vitro biocompatibility and bioactivity of calcium aluminate-PMMA composite cement. / Oh, S. H.; Choi, S. Y.; Choi, Seongho; Lee, Y. K.; Kim, K. N.

In: Journal of Materials Science: Materials in Medicine, Vol. 15, No. 1, 01.01.2004, p. 25-33.

Research output: Contribution to journalArticle

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T1 - The influence of lithium fluoride on in vitro biocompatibility and bioactivity of calcium aluminate-PMMA composite cement

AU - Oh, S. H.

AU - Choi, S. Y.

AU - Choi, Seongho

AU - Lee, Y. K.

AU - Kim, K. N.

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AB - The objective of this study is to assess the influence of lithium fluoride on in vitro biocompatibility and bioactivity of calcium aluminate (CA)-polymethylmethacrylate (PMMA) composite cement exhibiting quick setting time ( < 15 min), low exothermic temperature ( < 47°C), and high compressive strength ( > 100 MPa). The biocompatibility was measured by examining cytotoxicity tests such as the agar diffusion test with L929 cell line and the hemolysis test with fresh rabbit blood. To estimate the bioactivity of CA-PMMA composite cement, we determined hydroxyapatite (HAp) formation on the surface of composite cement in the simulated body (SBF) solution by using thin-film XRD, XPS, SEM, EPMA and ICP-AES. The results of biocompatibility tests indicated that all experimental compositions of this study had no cytotoxicity and no hemolysis so that there was no cytotoxicity with regard to non-reacted monomers (MMA and TEGDMA) and lithium fluoride. The results of bioactivity tests revealed that CA-PMMA composite cement without lithium fluoride did not form HAp on its surface after 60 days of soaking in the SBF. On the other hand, LiAl2(OH)7·2H 2O and HAp were formed on the surface of CA-PMMA composite cement including 1.0% by weight of lithium fluoride after 7 and 15 days of soaking in the SBF, respectively. The 5 μm of LiAl2(OH)7 ·2H2O and HAp mixed layers were formed on the surface of specimen after 60 days of soaking in the SBF.

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