The influences of β-glucan associated with BMP-7 on MC3T3-E1 proliferation and osteogenic differentiation

D. H. Lee, D. W. Han, B. J. Park, H. S. Baek, K. Takatori, M. Aihara, K. Tsubaki, J. C. Park

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

β-glucan, an immunomodulator, can selectively enhance the immunobiological activities of neutrophils and macrophages without stimulating proinflammatory cytokine production. Biologic response modifiers, like beta-glucan, will modulate immunity, modify neoplastic disease and increase resistance to microbial challenge. Therefore, beta-glucan polymers can be applied in bone induction and regeneration model and have a possibility of association with bone morphogenetic protein (BMP) because of tissue-regenerative and antimicrobial effects of those polymers. In this report, we studied an E. coli expression system for BMP-7 production and the biological activities of β-glucan associated with BMP-7. The proliferation of MC3T3-E1 osteoblastic cells was enhanced by treatment with Aureobasidium β-glucan, while neither mushroom β-glucan nor barley β-glucan increased the cell proliferation. Mushroom β-glucan alone or associated with BMP-7 increased alkaline phosphatase (ALP) activity of MC3T3-E1 cells, one of the osteoblast phenotype markers, but the other β-glucans did not affect ALP activity of the cells. In mineralization assay, a highly significant increase in nodular staining was observed in cultures treated with both mushroom and Aureobasidium β-glucans in the presence of BMP-7 compared with nontreated controls, while barley β-glucan showed a significant decrease in nodule number compared with cultures treated only with BMP-7.

Original languageEnglish
Pages (from-to)241-244
Number of pages4
JournalKey Engineering Materials
Volume288-289
Publication statusPublished - 2005 Dec 1

Fingerprint

Bone Morphogenetic Protein 7
Glucans
Bone
Proteins
Phosphatases
beta-Glucans
Alkaline Phosphatase
Polymers
Macrophages
Osteoblasts
Cell proliferation
Bioactivity
Bone Morphogenetic Proteins
Escherichia coli
Immunologic Factors
Assays
Cells
Association reactions
Tissue
Cytokines

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Lee, D. H., Han, D. W., Park, B. J., Baek, H. S., Takatori, K., Aihara, M., ... Park, J. C. (2005). The influences of β-glucan associated with BMP-7 on MC3T3-E1 proliferation and osteogenic differentiation. Key Engineering Materials, 288-289, 241-244.
Lee, D. H. ; Han, D. W. ; Park, B. J. ; Baek, H. S. ; Takatori, K. ; Aihara, M. ; Tsubaki, K. ; Park, J. C. / The influences of β-glucan associated with BMP-7 on MC3T3-E1 proliferation and osteogenic differentiation. In: Key Engineering Materials. 2005 ; Vol. 288-289. pp. 241-244.
@article{f2bdd1ee1bf14886aafa0ff982859796,
title = "The influences of β-glucan associated with BMP-7 on MC3T3-E1 proliferation and osteogenic differentiation",
abstract = "β-glucan, an immunomodulator, can selectively enhance the immunobiological activities of neutrophils and macrophages without stimulating proinflammatory cytokine production. Biologic response modifiers, like beta-glucan, will modulate immunity, modify neoplastic disease and increase resistance to microbial challenge. Therefore, beta-glucan polymers can be applied in bone induction and regeneration model and have a possibility of association with bone morphogenetic protein (BMP) because of tissue-regenerative and antimicrobial effects of those polymers. In this report, we studied an E. coli expression system for BMP-7 production and the biological activities of β-glucan associated with BMP-7. The proliferation of MC3T3-E1 osteoblastic cells was enhanced by treatment with Aureobasidium β-glucan, while neither mushroom β-glucan nor barley β-glucan increased the cell proliferation. Mushroom β-glucan alone or associated with BMP-7 increased alkaline phosphatase (ALP) activity of MC3T3-E1 cells, one of the osteoblast phenotype markers, but the other β-glucans did not affect ALP activity of the cells. In mineralization assay, a highly significant increase in nodular staining was observed in cultures treated with both mushroom and Aureobasidium β-glucans in the presence of BMP-7 compared with nontreated controls, while barley β-glucan showed a significant decrease in nodule number compared with cultures treated only with BMP-7.",
author = "Lee, {D. H.} and Han, {D. W.} and Park, {B. J.} and Baek, {H. S.} and K. Takatori and M. Aihara and K. Tsubaki and Park, {J. C.}",
year = "2005",
month = "12",
day = "1",
language = "English",
volume = "288-289",
pages = "241--244",
journal = "Key Engineering Materials",
issn = "1013-9826",
publisher = "Trans Tech Publications",

}

Lee, DH, Han, DW, Park, BJ, Baek, HS, Takatori, K, Aihara, M, Tsubaki, K & Park, JC 2005, 'The influences of β-glucan associated with BMP-7 on MC3T3-E1 proliferation and osteogenic differentiation', Key Engineering Materials, vol. 288-289, pp. 241-244.

The influences of β-glucan associated with BMP-7 on MC3T3-E1 proliferation and osteogenic differentiation. / Lee, D. H.; Han, D. W.; Park, B. J.; Baek, H. S.; Takatori, K.; Aihara, M.; Tsubaki, K.; Park, J. C.

In: Key Engineering Materials, Vol. 288-289, 01.12.2005, p. 241-244.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The influences of β-glucan associated with BMP-7 on MC3T3-E1 proliferation and osteogenic differentiation

AU - Lee, D. H.

AU - Han, D. W.

AU - Park, B. J.

AU - Baek, H. S.

AU - Takatori, K.

AU - Aihara, M.

AU - Tsubaki, K.

AU - Park, J. C.

PY - 2005/12/1

Y1 - 2005/12/1

N2 - β-glucan, an immunomodulator, can selectively enhance the immunobiological activities of neutrophils and macrophages without stimulating proinflammatory cytokine production. Biologic response modifiers, like beta-glucan, will modulate immunity, modify neoplastic disease and increase resistance to microbial challenge. Therefore, beta-glucan polymers can be applied in bone induction and regeneration model and have a possibility of association with bone morphogenetic protein (BMP) because of tissue-regenerative and antimicrobial effects of those polymers. In this report, we studied an E. coli expression system for BMP-7 production and the biological activities of β-glucan associated with BMP-7. The proliferation of MC3T3-E1 osteoblastic cells was enhanced by treatment with Aureobasidium β-glucan, while neither mushroom β-glucan nor barley β-glucan increased the cell proliferation. Mushroom β-glucan alone or associated with BMP-7 increased alkaline phosphatase (ALP) activity of MC3T3-E1 cells, one of the osteoblast phenotype markers, but the other β-glucans did not affect ALP activity of the cells. In mineralization assay, a highly significant increase in nodular staining was observed in cultures treated with both mushroom and Aureobasidium β-glucans in the presence of BMP-7 compared with nontreated controls, while barley β-glucan showed a significant decrease in nodule number compared with cultures treated only with BMP-7.

AB - β-glucan, an immunomodulator, can selectively enhance the immunobiological activities of neutrophils and macrophages without stimulating proinflammatory cytokine production. Biologic response modifiers, like beta-glucan, will modulate immunity, modify neoplastic disease and increase resistance to microbial challenge. Therefore, beta-glucan polymers can be applied in bone induction and regeneration model and have a possibility of association with bone morphogenetic protein (BMP) because of tissue-regenerative and antimicrobial effects of those polymers. In this report, we studied an E. coli expression system for BMP-7 production and the biological activities of β-glucan associated with BMP-7. The proliferation of MC3T3-E1 osteoblastic cells was enhanced by treatment with Aureobasidium β-glucan, while neither mushroom β-glucan nor barley β-glucan increased the cell proliferation. Mushroom β-glucan alone or associated with BMP-7 increased alkaline phosphatase (ALP) activity of MC3T3-E1 cells, one of the osteoblast phenotype markers, but the other β-glucans did not affect ALP activity of the cells. In mineralization assay, a highly significant increase in nodular staining was observed in cultures treated with both mushroom and Aureobasidium β-glucans in the presence of BMP-7 compared with nontreated controls, while barley β-glucan showed a significant decrease in nodule number compared with cultures treated only with BMP-7.

UR - http://www.scopus.com/inward/record.url?scp=34249703056&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34249703056&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:34249703056

VL - 288-289

SP - 241

EP - 244

JO - Key Engineering Materials

JF - Key Engineering Materials

SN - 1013-9826

ER -

Lee DH, Han DW, Park BJ, Baek HS, Takatori K, Aihara M et al. The influences of β-glucan associated with BMP-7 on MC3T3-E1 proliferation and osteogenic differentiation. Key Engineering Materials. 2005 Dec 1;288-289:241-244.