The inhibitory effect of honokiol, a natural plant product, on vestibular schwannoma cells

Jong Dae Lee, Jae Yong Lee, Byoung Joon Baek, Byung Don Lee, Yoonwoo Koh, Won Sang Lee, Yu Jin Lee, Byoung Mog Kwon

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objectives/Hypothesis: As the molecular biology of vestibular schwannoma (VS) is better understood, new means of targeting the pathways involved for intervention in schwannoma cells are being developed. Honokiol, a bioactive constituent of Magnolia officinalis, has attracted attention due to its diverse biological effects. This study was conducted to determine the inhibitory effect of honokiol on schwannoma cell proliferation. Methods: HEI 193 cells were used to investigate the growth-inhibitory effects of honokiol. Cell proliferation was assessed by MTT assays. Apoptosis was measured by flow cytometry analysis and immunofluorescence staining including Hoechst 33342 and TUNEL. Western blot analysis was used to assess the potential inhibition of extracellular signal-regulated kinase (ERK) and AKT signaling by honokiol. Results: Honokiol exhibited significant antiproliferative activity in a dose-dependent manner on HEI 193 cells. Significant apoptosis was detected on schwannoma cells with 7 mg/mL(IC 50 ) honokiol. Western blot analysis showed significant inhibition of ERK phosphorylation. Conclusions: Honokiol, a low molecular weight natural product, inhibits cell proliferation and promotes apoptosis in schwannoma cells by targeting the ERK pathway. Our data suggest that honokiol can be evaluated as a chemotherapeutic agent for VS.

Original languageEnglish
Pages (from-to)162-166
Number of pages5
JournalLaryngoscope
Volume122
Issue number1
DOIs
Publication statusPublished - 2012 Jan 1

Fingerprint

Acoustic Neuroma
Neurilemmoma
Extracellular Signal-Regulated MAP Kinases
Cell Proliferation
Apoptosis
Western Blotting
Magnolia
honokiol
In Situ Nick-End Labeling
Biological Products
Fluorescent Antibody Technique
Molecular Biology
Flow Cytometry
Molecular Weight
Phosphorylation
Staining and Labeling
Growth

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

Cite this

Lee, J. D., Lee, J. Y., Baek, B. J., Lee, B. D., Koh, Y., Lee, W. S., ... Kwon, B. M. (2012). The inhibitory effect of honokiol, a natural plant product, on vestibular schwannoma cells. Laryngoscope, 122(1), 162-166. https://doi.org/10.1002/lary.21781
Lee, Jong Dae ; Lee, Jae Yong ; Baek, Byoung Joon ; Lee, Byung Don ; Koh, Yoonwoo ; Lee, Won Sang ; Lee, Yu Jin ; Kwon, Byoung Mog. / The inhibitory effect of honokiol, a natural plant product, on vestibular schwannoma cells. In: Laryngoscope. 2012 ; Vol. 122, No. 1. pp. 162-166.
@article{c70875cde5274da888499ef283e0e793,
title = "The inhibitory effect of honokiol, a natural plant product, on vestibular schwannoma cells",
abstract = "Objectives/Hypothesis: As the molecular biology of vestibular schwannoma (VS) is better understood, new means of targeting the pathways involved for intervention in schwannoma cells are being developed. Honokiol, a bioactive constituent of Magnolia officinalis, has attracted attention due to its diverse biological effects. This study was conducted to determine the inhibitory effect of honokiol on schwannoma cell proliferation. Methods: HEI 193 cells were used to investigate the growth-inhibitory effects of honokiol. Cell proliferation was assessed by MTT assays. Apoptosis was measured by flow cytometry analysis and immunofluorescence staining including Hoechst 33342 and TUNEL. Western blot analysis was used to assess the potential inhibition of extracellular signal-regulated kinase (ERK) and AKT signaling by honokiol. Results: Honokiol exhibited significant antiproliferative activity in a dose-dependent manner on HEI 193 cells. Significant apoptosis was detected on schwannoma cells with 7 mg/mL(IC 50 ) honokiol. Western blot analysis showed significant inhibition of ERK phosphorylation. Conclusions: Honokiol, a low molecular weight natural product, inhibits cell proliferation and promotes apoptosis in schwannoma cells by targeting the ERK pathway. Our data suggest that honokiol can be evaluated as a chemotherapeutic agent for VS.",
author = "Lee, {Jong Dae} and Lee, {Jae Yong} and Baek, {Byoung Joon} and Lee, {Byung Don} and Yoonwoo Koh and Lee, {Won Sang} and Lee, {Yu Jin} and Kwon, {Byoung Mog}",
year = "2012",
month = "1",
day = "1",
doi = "10.1002/lary.21781",
language = "English",
volume = "122",
pages = "162--166",
journal = "Laryngoscope",
issn = "0023-852X",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

Lee, JD, Lee, JY, Baek, BJ, Lee, BD, Koh, Y, Lee, WS, Lee, YJ & Kwon, BM 2012, 'The inhibitory effect of honokiol, a natural plant product, on vestibular schwannoma cells', Laryngoscope, vol. 122, no. 1, pp. 162-166. https://doi.org/10.1002/lary.21781

The inhibitory effect of honokiol, a natural plant product, on vestibular schwannoma cells. / Lee, Jong Dae; Lee, Jae Yong; Baek, Byoung Joon; Lee, Byung Don; Koh, Yoonwoo; Lee, Won Sang; Lee, Yu Jin; Kwon, Byoung Mog.

In: Laryngoscope, Vol. 122, No. 1, 01.01.2012, p. 162-166.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The inhibitory effect of honokiol, a natural plant product, on vestibular schwannoma cells

AU - Lee, Jong Dae

AU - Lee, Jae Yong

AU - Baek, Byoung Joon

AU - Lee, Byung Don

AU - Koh, Yoonwoo

AU - Lee, Won Sang

AU - Lee, Yu Jin

AU - Kwon, Byoung Mog

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Objectives/Hypothesis: As the molecular biology of vestibular schwannoma (VS) is better understood, new means of targeting the pathways involved for intervention in schwannoma cells are being developed. Honokiol, a bioactive constituent of Magnolia officinalis, has attracted attention due to its diverse biological effects. This study was conducted to determine the inhibitory effect of honokiol on schwannoma cell proliferation. Methods: HEI 193 cells were used to investigate the growth-inhibitory effects of honokiol. Cell proliferation was assessed by MTT assays. Apoptosis was measured by flow cytometry analysis and immunofluorescence staining including Hoechst 33342 and TUNEL. Western blot analysis was used to assess the potential inhibition of extracellular signal-regulated kinase (ERK) and AKT signaling by honokiol. Results: Honokiol exhibited significant antiproliferative activity in a dose-dependent manner on HEI 193 cells. Significant apoptosis was detected on schwannoma cells with 7 mg/mL(IC 50 ) honokiol. Western blot analysis showed significant inhibition of ERK phosphorylation. Conclusions: Honokiol, a low molecular weight natural product, inhibits cell proliferation and promotes apoptosis in schwannoma cells by targeting the ERK pathway. Our data suggest that honokiol can be evaluated as a chemotherapeutic agent for VS.

AB - Objectives/Hypothesis: As the molecular biology of vestibular schwannoma (VS) is better understood, new means of targeting the pathways involved for intervention in schwannoma cells are being developed. Honokiol, a bioactive constituent of Magnolia officinalis, has attracted attention due to its diverse biological effects. This study was conducted to determine the inhibitory effect of honokiol on schwannoma cell proliferation. Methods: HEI 193 cells were used to investigate the growth-inhibitory effects of honokiol. Cell proliferation was assessed by MTT assays. Apoptosis was measured by flow cytometry analysis and immunofluorescence staining including Hoechst 33342 and TUNEL. Western blot analysis was used to assess the potential inhibition of extracellular signal-regulated kinase (ERK) and AKT signaling by honokiol. Results: Honokiol exhibited significant antiproliferative activity in a dose-dependent manner on HEI 193 cells. Significant apoptosis was detected on schwannoma cells with 7 mg/mL(IC 50 ) honokiol. Western blot analysis showed significant inhibition of ERK phosphorylation. Conclusions: Honokiol, a low molecular weight natural product, inhibits cell proliferation and promotes apoptosis in schwannoma cells by targeting the ERK pathway. Our data suggest that honokiol can be evaluated as a chemotherapeutic agent for VS.

UR - http://www.scopus.com/inward/record.url?scp=84355162073&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84355162073&partnerID=8YFLogxK

U2 - 10.1002/lary.21781

DO - 10.1002/lary.21781

M3 - Article

C2 - 22057812

AN - SCOPUS:84355162073

VL - 122

SP - 162

EP - 166

JO - Laryngoscope

JF - Laryngoscope

SN - 0023-852X

IS - 1

ER -