Abstract
Objectives/Hypothesis: As the molecular biology of vestibular schwannoma (VS) is better understood, new means of targeting the pathways involved for intervention in schwannoma cells are being developed. Honokiol, a bioactive constituent of Magnolia officinalis, has attracted attention due to its diverse biological effects. This study was conducted to determine the inhibitory effect of honokiol on schwannoma cell proliferation. Methods: HEI 193 cells were used to investigate the growth-inhibitory effects of honokiol. Cell proliferation was assessed by MTT assays. Apoptosis was measured by flow cytometry analysis and immunofluorescence staining including Hoechst 33342 and TUNEL. Western blot analysis was used to assess the potential inhibition of extracellular signal-regulated kinase (ERK) and AKT signaling by honokiol. Results: Honokiol exhibited significant antiproliferative activity in a dose-dependent manner on HEI 193 cells. Significant apoptosis was detected on schwannoma cells with 7 mg/mL(IC 50 ) honokiol. Western blot analysis showed significant inhibition of ERK phosphorylation. Conclusions: Honokiol, a low molecular weight natural product, inhibits cell proliferation and promotes apoptosis in schwannoma cells by targeting the ERK pathway. Our data suggest that honokiol can be evaluated as a chemotherapeutic agent for VS.
| Original language | English |
|---|---|
| Pages (from-to) | 162-166 |
| Number of pages | 5 |
| Journal | Laryngoscope |
| Volume | 122 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2012 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Otorhinolaryngology
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The inhibitory effect of honokiol, a natural plant product, on vestibular schwannoma cells. / Lee, Jong Dae; Lee, Jae Yong; Baek, Byoung Joon; Lee, Byung Don; Koh, Yoonwoo; Lee, Won Sang; Lee, Yu Jin; Kwon, Byoung Mog.
In: Laryngoscope, Vol. 122, No. 1, 01.01.2012, p. 162-166.Research output: Contribution to journal › Article
TY - JOUR
T1 - The inhibitory effect of honokiol, a natural plant product, on vestibular schwannoma cells
AU - Lee, Jong Dae
AU - Lee, Jae Yong
AU - Baek, Byoung Joon
AU - Lee, Byung Don
AU - Koh, Yoonwoo
AU - Lee, Won Sang
AU - Lee, Yu Jin
AU - Kwon, Byoung Mog
PY - 2012/1/1
Y1 - 2012/1/1
N2 - Objectives/Hypothesis: As the molecular biology of vestibular schwannoma (VS) is better understood, new means of targeting the pathways involved for intervention in schwannoma cells are being developed. Honokiol, a bioactive constituent of Magnolia officinalis, has attracted attention due to its diverse biological effects. This study was conducted to determine the inhibitory effect of honokiol on schwannoma cell proliferation. Methods: HEI 193 cells were used to investigate the growth-inhibitory effects of honokiol. Cell proliferation was assessed by MTT assays. Apoptosis was measured by flow cytometry analysis and immunofluorescence staining including Hoechst 33342 and TUNEL. Western blot analysis was used to assess the potential inhibition of extracellular signal-regulated kinase (ERK) and AKT signaling by honokiol. Results: Honokiol exhibited significant antiproliferative activity in a dose-dependent manner on HEI 193 cells. Significant apoptosis was detected on schwannoma cells with 7 mg/mL(IC 50 ) honokiol. Western blot analysis showed significant inhibition of ERK phosphorylation. Conclusions: Honokiol, a low molecular weight natural product, inhibits cell proliferation and promotes apoptosis in schwannoma cells by targeting the ERK pathway. Our data suggest that honokiol can be evaluated as a chemotherapeutic agent for VS.
AB - Objectives/Hypothesis: As the molecular biology of vestibular schwannoma (VS) is better understood, new means of targeting the pathways involved for intervention in schwannoma cells are being developed. Honokiol, a bioactive constituent of Magnolia officinalis, has attracted attention due to its diverse biological effects. This study was conducted to determine the inhibitory effect of honokiol on schwannoma cell proliferation. Methods: HEI 193 cells were used to investigate the growth-inhibitory effects of honokiol. Cell proliferation was assessed by MTT assays. Apoptosis was measured by flow cytometry analysis and immunofluorescence staining including Hoechst 33342 and TUNEL. Western blot analysis was used to assess the potential inhibition of extracellular signal-regulated kinase (ERK) and AKT signaling by honokiol. Results: Honokiol exhibited significant antiproliferative activity in a dose-dependent manner on HEI 193 cells. Significant apoptosis was detected on schwannoma cells with 7 mg/mL(IC 50 ) honokiol. Western blot analysis showed significant inhibition of ERK phosphorylation. Conclusions: Honokiol, a low molecular weight natural product, inhibits cell proliferation and promotes apoptosis in schwannoma cells by targeting the ERK pathway. Our data suggest that honokiol can be evaluated as a chemotherapeutic agent for VS.
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UR - http://www.scopus.com/inward/citedby.url?scp=84355162073&partnerID=8YFLogxK
U2 - 10.1002/lary.21781
DO - 10.1002/lary.21781
M3 - Article
C2 - 22057812
AN - SCOPUS:84355162073
VL - 122
SP - 162
EP - 166
JO - Laryngoscope
JF - Laryngoscope
SN - 0023-852X
IS - 1
ER -