The interaction of hepatitis B virus X protein and protein phosphatase type 2 Cα and its effect on IL-6

Ji Su Kim, Bo young Rho, Tae Ho Lee, Jae Myun Lee, Se Jong Kim, Jeon Han Park

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

HBx has been suggested as an important determinant mediating the pathological effects of HBV via interacting with various cellular proteins. To identify new HBx-interacting proteins and elucidate a possible mechanism associated with HBx and HBx-interacting proteins in hepatocellular carcinoma, yeast two-hybrid screening was performed. We identified a novel HBx-interacting protein, serine/threonine protein phosphatase PP2Cα, and investigated the effects of PP2Cα on HBx-mediated IL-6 regulation. The interaction between endogenous PP2Cα, and HBx was confirmed by co-immunoprecipitation. Recombinant HBx dose-dependently reduced enzyme activity of recombinant PP2Cα in vitro. While ectopically expressed PP2Cα in Cos-7 and Huh-7 cells reduced the expression of IL-6, overexpressed HBx with recombinant HBx-expressing adenovirus overcame PP2Cα-mediated IL-6 downregulation. In the response of IL-6, HBx phosphorylated STAT3 and recovered PP2Cα-mediated dephosphorylation of STAT3. These results supported that HBx might play a crucial role in HBV-associated hepatocarcinogenesis even in cases where cells express a negative regulator, PP2Cα.

Original languageEnglish
Pages (from-to)253-258
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume351
Issue number1
DOIs
Publication statusPublished - 2006 Dec 8

Bibliographical note

Funding Information:
This work was supported by the Brain Korea 21 Project in 2005.

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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