The interaction of hepatitis B virus X protein and protein phosphatase type 2 Cα and its effect on IL-6

Ji Su Kim, Bo young Rho, Tae Ho Lee, Jae Myun Lee, Se Jong Kim, Jeon Han Park

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

HBx has been suggested as an important determinant mediating the pathological effects of HBV via interacting with various cellular proteins. To identify new HBx-interacting proteins and elucidate a possible mechanism associated with HBx and HBx-interacting proteins in hepatocellular carcinoma, yeast two-hybrid screening was performed. We identified a novel HBx-interacting protein, serine/threonine protein phosphatase PP2Cα, and investigated the effects of PP2Cα on HBx-mediated IL-6 regulation. The interaction between endogenous PP2Cα, and HBx was confirmed by co-immunoprecipitation. Recombinant HBx dose-dependently reduced enzyme activity of recombinant PP2Cα in vitro. While ectopically expressed PP2Cα in Cos-7 and Huh-7 cells reduced the expression of IL-6, overexpressed HBx with recombinant HBx-expressing adenovirus overcame PP2Cα-mediated IL-6 downregulation. In the response of IL-6, HBx phosphorylated STAT3 and recovered PP2Cα-mediated dephosphorylation of STAT3. These results supported that HBx might play a crucial role in HBV-associated hepatocarcinogenesis even in cases where cells express a negative regulator, PP2Cα.

Original languageEnglish
Pages (from-to)253-258
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume351
Issue number1
DOIs
Publication statusPublished - 2006 Dec 8

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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