The intraoperative immunohistochemical staining of CD56 and CK19 improves surgical decision for thyroid follicular lesions

Ju Yeon Pyo, Sung Eun Choi, Eunah Shin, Ja Seung Koo, Soon Won Hong

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: When differential diagnosis is difficult in thyroid follicular lesions with overlapping histological features, the immunohistochemical staining can help confirm the diagnosis. We aimed to evaluate the effectiveness of rapid immunohistochemical stains of CD56 and cytokeratin 19 on frozen sections of thyroid follicular lesion and explore the possible gains and limitations of the practice. Methods: Eighty-six nodules of 79 patients whose intraoperative frozen sections were selected as the control group, and 53 nodules of 48 patients whose intraoperative frozen sections were subject to rapid immunohistochemistry were selected as the study group. Results: Five nodules (6%) in the control group were diagnosed as follicular neoplasm and six nodules (7%) were deferred. In the study group, six nodules (11%) were follicular neoplasm and none were deferred. Three nodules (4%) in the control group showed diagnostic discrepancy between the frozen and permanent diagnoses, but none in the study group. The average turnaround time for the frozen diagnosis of the control group was 24 minutes, whereas it was 54 minutes for the study group. Conclusions: Intraoperative rapid immunohistochemical stains significantly decreased the diagnostic discrepancy in this study. Considering the adverse effects of indefinite frozen diagnosis or discrepancy with permanent diagnoses, the intraoperative rapid immunohistochemical stain can help to accurately diagnose and hence provide guidance to surgical treatment.

Original languageEnglish
Pages (from-to)463-470
Number of pages8
JournalJournal of Pathology and Translational Medicine
Volume51
Issue number5
DOIs
Publication statusPublished - 2017 Jan 1

Fingerprint

Thyroid Gland
Staining and Labeling
Frozen Sections
Control Groups
Coloring Agents
Keratin-19
Neoplasms
Differential Diagnosis
Immunohistochemistry
Therapeutics

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology

Cite this

@article{744640a5434c493fa78fd86c584a95ac,
title = "The intraoperative immunohistochemical staining of CD56 and CK19 improves surgical decision for thyroid follicular lesions",
abstract = "Background: When differential diagnosis is difficult in thyroid follicular lesions with overlapping histological features, the immunohistochemical staining can help confirm the diagnosis. We aimed to evaluate the effectiveness of rapid immunohistochemical stains of CD56 and cytokeratin 19 on frozen sections of thyroid follicular lesion and explore the possible gains and limitations of the practice. Methods: Eighty-six nodules of 79 patients whose intraoperative frozen sections were selected as the control group, and 53 nodules of 48 patients whose intraoperative frozen sections were subject to rapid immunohistochemistry were selected as the study group. Results: Five nodules (6{\%}) in the control group were diagnosed as follicular neoplasm and six nodules (7{\%}) were deferred. In the study group, six nodules (11{\%}) were follicular neoplasm and none were deferred. Three nodules (4{\%}) in the control group showed diagnostic discrepancy between the frozen and permanent diagnoses, but none in the study group. The average turnaround time for the frozen diagnosis of the control group was 24 minutes, whereas it was 54 minutes for the study group. Conclusions: Intraoperative rapid immunohistochemical stains significantly decreased the diagnostic discrepancy in this study. Considering the adverse effects of indefinite frozen diagnosis or discrepancy with permanent diagnoses, the intraoperative rapid immunohistochemical stain can help to accurately diagnose and hence provide guidance to surgical treatment.",
author = "Pyo, {Ju Yeon} and Choi, {Sung Eun} and Eunah Shin and Koo, {Ja Seung} and Hong, {Soon Won}",
year = "2017",
month = "1",
day = "1",
doi = "10.4132/jptm.2017.05.25",
language = "English",
volume = "51",
pages = "463--470",
journal = "Journal of Pathology and Translational Medicine",
issn = "2383-7837",
publisher = "Korean Society of Pathologists",
number = "5",

}

The intraoperative immunohistochemical staining of CD56 and CK19 improves surgical decision for thyroid follicular lesions. / Pyo, Ju Yeon; Choi, Sung Eun; Shin, Eunah; Koo, Ja Seung; Hong, Soon Won.

In: Journal of Pathology and Translational Medicine, Vol. 51, No. 5, 01.01.2017, p. 463-470.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The intraoperative immunohistochemical staining of CD56 and CK19 improves surgical decision for thyroid follicular lesions

AU - Pyo, Ju Yeon

AU - Choi, Sung Eun

AU - Shin, Eunah

AU - Koo, Ja Seung

AU - Hong, Soon Won

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Background: When differential diagnosis is difficult in thyroid follicular lesions with overlapping histological features, the immunohistochemical staining can help confirm the diagnosis. We aimed to evaluate the effectiveness of rapid immunohistochemical stains of CD56 and cytokeratin 19 on frozen sections of thyroid follicular lesion and explore the possible gains and limitations of the practice. Methods: Eighty-six nodules of 79 patients whose intraoperative frozen sections were selected as the control group, and 53 nodules of 48 patients whose intraoperative frozen sections were subject to rapid immunohistochemistry were selected as the study group. Results: Five nodules (6%) in the control group were diagnosed as follicular neoplasm and six nodules (7%) were deferred. In the study group, six nodules (11%) were follicular neoplasm and none were deferred. Three nodules (4%) in the control group showed diagnostic discrepancy between the frozen and permanent diagnoses, but none in the study group. The average turnaround time for the frozen diagnosis of the control group was 24 minutes, whereas it was 54 minutes for the study group. Conclusions: Intraoperative rapid immunohistochemical stains significantly decreased the diagnostic discrepancy in this study. Considering the adverse effects of indefinite frozen diagnosis or discrepancy with permanent diagnoses, the intraoperative rapid immunohistochemical stain can help to accurately diagnose and hence provide guidance to surgical treatment.

AB - Background: When differential diagnosis is difficult in thyroid follicular lesions with overlapping histological features, the immunohistochemical staining can help confirm the diagnosis. We aimed to evaluate the effectiveness of rapid immunohistochemical stains of CD56 and cytokeratin 19 on frozen sections of thyroid follicular lesion and explore the possible gains and limitations of the practice. Methods: Eighty-six nodules of 79 patients whose intraoperative frozen sections were selected as the control group, and 53 nodules of 48 patients whose intraoperative frozen sections were subject to rapid immunohistochemistry were selected as the study group. Results: Five nodules (6%) in the control group were diagnosed as follicular neoplasm and six nodules (7%) were deferred. In the study group, six nodules (11%) were follicular neoplasm and none were deferred. Three nodules (4%) in the control group showed diagnostic discrepancy between the frozen and permanent diagnoses, but none in the study group. The average turnaround time for the frozen diagnosis of the control group was 24 minutes, whereas it was 54 minutes for the study group. Conclusions: Intraoperative rapid immunohistochemical stains significantly decreased the diagnostic discrepancy in this study. Considering the adverse effects of indefinite frozen diagnosis or discrepancy with permanent diagnoses, the intraoperative rapid immunohistochemical stain can help to accurately diagnose and hence provide guidance to surgical treatment.

UR - http://www.scopus.com/inward/record.url?scp=85029795577&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85029795577&partnerID=8YFLogxK

U2 - 10.4132/jptm.2017.05.25

DO - 10.4132/jptm.2017.05.25

M3 - Article

AN - SCOPUS:85029795577

VL - 51

SP - 463

EP - 470

JO - Journal of Pathology and Translational Medicine

JF - Journal of Pathology and Translational Medicine

SN - 2383-7837

IS - 5

ER -